EMAGE mouse gene expression spatial database

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EMAGE:10251

Atp7b ATPase, Cu++ transporting, beta polypeptide ( MGI:103297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018905. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018905_01	euxassay_018905_02	euxassay_018905_03	euxassay_018905_04

euxassay_018905_05	euxassay_018905_06	euxassay_018905_07	euxassay_018905_08	euxassay_018905_09

euxassay_018905_10	euxassay_018905_11	euxassay_018905_12	euxassay_018905_13	euxassay_018905_14

euxassay_018905_15	euxassay_018905_16	euxassay_018905_17	euxassay_018905_18	euxassay_018905_19

euxassay_018905_20	euxassay_018905_21	euxassay_018905_22	euxassay_018905_23	euxassay_018905_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10251_wholemount_strong.wlz

10251_wholemount_moderate.wlz

10251_wholemount_weak.wlz

10251_wholemount_possible.wlz

10251_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10251_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 11 12

pancreas

weak

regional

weak expression: see section 06 07 08 09 10 11 12 15

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 16 17 18 19

esophagus

weak

regional

weak expression: see section 12 13 14

stomach

strong

regional

strong expression: see section 06 07 moderate expression: see section 05 08 09 10 weak expression: see section 03 04 11

midgut

weak

regional

weak expression: see section 09 10 11 13 14 15 17

liver

strong

regional

strong expression: see section 01 02 03 06 07 11 13 14 15 17 18 19 21 moderate expression: see section 04 05 08 10 12 16 20 22 23 24

lung

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

trachea

weak

regional

weak expression: see section 13 14

nucleus pulposus

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50130
Entity Detected:	Atp7b, ATPase, Cu++ transporting, beta polypeptide ( MGI:103297)
Sequence:	sense strand is shown >T50130 TTCGCCTTCGACAATGTTGGCTACGAAGGGGGTCTGGACAGCACCAGCTCGTCCCCAGCAGCCACTGATG TGGTTAACATCCTGGGCATGACTTGTCATTCTTGCGTCAAGTCCATTGAGGACAGGATCTCCAGTCTGAA AGGCATTGTGAACATCAAGGTGTCCCTGGAACAAGGCAAGCACACTGTCAGATATGTGCCGTCTGTCATG AACCTGCAGCAGATTTGCCTTCAGATTGAGGATATGGGCTTTGAGGCCAGCGCTGCAGAGGGAAAGGCCG CCTCCTGGCCTTCCAGGTCCTCCCCAGCCCAGGAGGCGGTGGTCAAGCTCCGGGTAGAGGGCATGACCTG TCAGTCCTGTGTCAGCTCTATCGAGGGCAAGATCCGGAAACTGCAAGGGGTTGTGAGAATCAAAGTCTCC CTAAGCAACCAAGAGGCAGTCATTACCTATCAGCCTTACCTCATTCAACCCGAAGACCTCAGGGACCACA TCTGTGACATGGGATTTGAAGCTGCCATCAAGAACAGAACAGCTCCCTTAAGGCTGGGACCAATTGATGT CAACAAGTTAGAAAGCACTAACCTAAAGAAAGAGACGGTCTCTCCTGTCCAGATTTCCAATCATTTTGAG ACCCTGGGGCACCAGGGGAGCTACCTGGCCACCCTCCCACTAAGAATAGACGGCATGCACTGTAAATCAT GTGTTTTGAATATTGAAGGAAATATAGGCCAACTCCCGGGGGTTCAAAATATTCACGTGTCCTTGGAGAA CAAAACCGCCCAAATACAGTATGACCCTTCTTGTGTCACCCCCATGTTCCTGCAGACAGCCATCGAGGCA CTACCACCTGGGCACTTT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTCGCCTTCGACAATGTTGG; Reverse Primer - name:unspecified, sequence:AAAGTGCCCAGGTGGTAGTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10252 same embryo
	EMAGE:10248 same embryo
	EMAGE:10247 same embryo
	EMAGE:10250 same embryo
	EMAGE:10249 same embryo
	EurExpress:euxassay_018905 same experiment
	MGI:4823342 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source