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EMAGE:10301

Gapvd1 GTPase activating protein and VPS9 domains 1 ( MGI:1913941)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301
"Pseudo-wholemount" of euxassay_000318. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000318_01 euxassay_000318_02 euxassay_000318_03 euxassay_000318_04
EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301
euxassay_000318_05 euxassay_000318_06 euxassay_000318_07 euxassay_000318_08 euxassay_000318_09
EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301
euxassay_000318_10 euxassay_000318_11 euxassay_000318_12 euxassay_000318_13 euxassay_000318_14
EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301
euxassay_000318_15 euxassay_000318_16 euxassay_000318_17 euxassay_000318_18 euxassay_000318_19
EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301 EMAGE:10301
euxassay_000318_20 euxassay_000318_21 euxassay_000318_22 euxassay_000318_23 euxassay_000318_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10301Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10301_wholemount_strong.wlz
10301_wholemount_moderate.wlz
10301_wholemount_weak.wlz
10301_wholemount_possible.wlz
10301_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10301_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 11 weak expression: see section 12 14
nervous system
weak weak
homogeneousweak expression: see section 12 13 14 15 17
central nervous system
moderate moderate
homogeneousmoderate expression: see section 19
telencephalon
moderate moderate
homogeneousmoderate expression: see section 19 20 21 22 weak expression: see section 18
facial vii ganglion
moderate moderate
homogeneousmoderate expression: see section 17
glossopharyngeal ix ganglion
moderate moderate
homogeneousmoderate expression: see section 07 17
trigeminal v ganglion
moderate moderate
homogeneousmoderate expression: see section 03 04 06 07 08 17 18 20 weak expression: see section 16
vagus x ganglion
moderate moderate
homogeneousmoderate expression: see section 07 08 09 17 18
vestibulocochlear viii ganglion
moderate moderate
homogeneousmoderate expression: see section 07 09 11 17
spinal cord
moderate moderate
homogeneousmoderate expression: see section 10
dorsal root ganglion
moderate moderate
homogeneousmoderate expression: see section 08 09 10 11 17 18
inner ear
moderate moderate
regionalmoderate expression: see section 22
metanephros
moderate moderate
regionalmoderate expression: see section 08 09 19 weak expression: see section 06 07
nucleus pulposus
moderate moderate
homogeneousmoderate expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3552
Entity Detected:Gapvd1, GTPase activating protein and VPS9 domains 1 ( MGI:1913941)
Sequence:sense strand is shown

>T3552
GGCCTCNAGGCCAGATTCGGAACGAGGAAAACCAGAACAGAAATCTTTTAGGAAACGTCTAGATCTTGGT
TCATGAATTCCCTGTTCGTCCTTCTCATTTTTAGAAAGAACAGTGTGCCCCTTCCAAGTTGCTGTGTGAG
CGTTCGGTCGTGAGGCACCTGCACGGAGGGTCTCAGGAGATGCGCAATACGGGCTCCCTAGATACACTCT
CCTAAGGAAGAGCTAAGAAGGAAATGTAAATCTAATATATATTTATATTTGATGTACTATGGGCATCTTC
AGACTCTAATAAACAACAAGGACAGTTGCTGAGAGTAGGCTGTGACATTCCCTCTTGTGAAATGGTTTCC
TTGACAAAATTTAAGCTGAGCTTAAAAGCAAAAGCAGAAATATTTATTAGTCTGGAGTAGAGTTTATCGC
TCTGCATGTGTGGAGTTTGATGGGGAAGTCATGAAGGCGTGTACCACCATGGACATGTGTTGAGCCTGCG
CATTGGGGCAGACTCCACCATGTGGACAAGCAGTACGTGGCATGGCAGGTGTGCTCATGCGTCCTTCCTT
TGCACCTGCGCACTGAG
Notes:The probe template was PCR amplified from IMAGE:3166837 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3166837 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10302 same embryo
 EMAGE:10298 same embryo
 EMAGE:10303 same embryo
 EMAGE:10299 same embryo
 EMAGE:10300 same embryo
 EurExpress:euxassay_000318 same experiment
 MGI:4824994 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS