EMAGE mouse gene expression spatial database

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EMAGE:10302

Asrgl1 asparaginase like 1 ( MGI:1913764)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000035. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000035_01	euxassay_000035_02	euxassay_000035_03	euxassay_000035_04

euxassay_000035_05	euxassay_000035_06	euxassay_000035_07	euxassay_000035_08	euxassay_000035_09

euxassay_000035_10	euxassay_000035_11	euxassay_000035_12	euxassay_000035_13	euxassay_000035_14

euxassay_000035_15	euxassay_000035_16	euxassay_000035_17	euxassay_000035_18	euxassay_000035_19

euxassay_000035_20	euxassay_000035_21	euxassay_000035_22	euxassay_000035_23	euxassay_000035_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10302_wholemount_strong.wlz

10302_wholemount_moderate.wlz

10302_wholemount_weak.wlz

10302_wholemount_possible.wlz

10302_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10302_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

central nervous system

strong

homogeneous

strong expression: see section 07 08 moderate expression: see section 06 weak expression: see section 01 02 03 04 05

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 11 moderate expression: see section 12 13

cerebral cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 weak expression: see section 09 10 11 14 15 16 18 19 20 21 22

corpus striatum

moderate

homogeneous

moderate expression: see section 14 16 18 19 20 21 22 weak expression: see section 09 10 11 15

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 10

medulla oblongata basal plate ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 moderate expression: see section 12 13 14

pons ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 moderate expression: see section 12 13 14 15

midbrain ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 14 moderate expression: see section 12 15

spinal cord ventricular layer

strong

homogeneous

strong expression: see section 11 14 moderate expression: see section 12 weak expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3550
Entity Detected:	Asrgl1, asparaginase like 1 ( MGI:1913764)
Sequence:	sense strand is shown >T3550 GTGGACCTCTGCCTCCTGCCCTGGGCAGCGGTGAAGAATGGCAAGCTGCAGGCCGGCATTGACCTCTGTG AGACCAGGACAAGGGACCTACCTTGCTAGCCTGGACGTGAAGCTGAGCTACCTGGTGTGAAGACCTAACT TAATCAATTAGATGTAGACATGGAAAACTTACGCAGTCTGTCCCTCCTTTTGTTGCCTTGATCAGTAAAT CAATGAATGCTTGGTTGAGGGTCGGGTTCTGAAGCGATGAGAGATGCCTGCCTGTATTAAGACCAGGGAA AATGGAAGCCTTGAGGAGCCTACATCTCCATCATGTAAGGCTAGAAAGGAGGTGTTAGATTTTGACCCAC GGAACTGCCTCTGAGCTATAGAAGTGCCATTTGGATTGGGAGAATGGCACTTTGCAGAGGCTAGGCTAGG GGAGTGTGGGAAGAAGGACTGAAAAAAAAAAGTGTAAGAAAGACCCAGGTCCAGGTGAAGCTCAAAATGT TACAGAGTTCATTCTTAGTCACAGGAAC
Notes:	The probe template was PCR amplified from IMAGE:3166802 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3166802 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10298 same embryo
	EMAGE:10301 same embryo
	EMAGE:10303 same embryo
	EMAGE:10299 same embryo
	EMAGE:10300 same embryo
	EurExpress:euxassay_000035 same experiment
	MGI:4823282 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source