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EMAGE:10728

Ift27 intraflagellar transport 27 homolog (Chlamydomonas) ( MGI:1914292)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728
"Pseudo-wholemount" of euxassay_003000. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003000_01 euxassay_003000_02 euxassay_003000_03 euxassay_003000_04
EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728
euxassay_003000_05 euxassay_003000_06 euxassay_003000_07 euxassay_003000_08 euxassay_003000_09
EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728
euxassay_003000_10 euxassay_003000_11 euxassay_003000_12 euxassay_003000_13 euxassay_003000_14
EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728
euxassay_003000_15 euxassay_003000_16 euxassay_003000_17 euxassay_003000_18 euxassay_003000_19
EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728 EMAGE:10728
euxassay_003000_20 euxassay_003000_21 euxassay_003000_22 euxassay_003000_23 euxassay_003000_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10728Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10728_wholemount_strong.wlz
10728_wholemount_moderate.wlz
10728_wholemount_weak.wlz
10728_wholemount_possible.wlz
10728_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10728_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4241
Entity Detected:Ift27, intraflagellar transport 27 homolog (Chlamydomonas) ( MGI:1914292)
Sequence:sense strand is shown

>T4241
GAAGGCGGGTTGATGATGGTTGCCAGGAGCCTGTTTGGCGCGACTCTTGGGACCAGAGGACTAGTGGTTG
TGTCTCAACTCTTCTTTGGGCCGGGTCTAAGGCTGGACTTGGAACGGGTTCTGCTCCGCCCCACCGGAAC
CTTTGCAGGCTGCCTGGGGCCTGAAGCCCTCCGTCCACCCAACCCTAGCACCCGGGCCATCATCCATCCC
TCCATCACGGCCTCAGGCCCTGGTGTCAGCTGTCCCCGAATCCTAACCCCCTTTCAGCGTCCCCCACCCC
CACGTCTCCGCTAGTACCTGGTTACTATGGTGAAGCTAGCTGCCAAATGCATCCTGGCAGGGGACCCAGC
CGTAGGCAAGACGGCCCTGGTGCAGATGTTCCGCAGCGACGGGACCCATTTCCAGAAGAACTACACCCTG
ACAACTGGAGTGGATTTGGTGGTGAAGACAGTGCCAGTTCTTGACACAAATGACAGTGTGGAACTCTTCA
TTTTTGACTCAGCCGGCAAGGAGCTGTTCTCTGAAATGCTGGATAAGTTGTGGGAGAATCCCAACGTCCT
GTGCCTTGTCTATGATGTGACCAATGAACAGTCCTTCATCAGCTGCACCAAGTGGTTGGAGAAGGTCCGG
TCTCAGACTTCAGGCATCTCCCTCCCAGGTGTTTTAGTGGGAACTAAGACAGACCTGGCTGGCCGACAAA
CAGTGGATTCAGCTCAGGCCCAGGTATGGGCACTGAGTCAAGGCCTGGAATTCTTTGAGACATCTGTGAA
GGAGATGGATAATTACGAGGCCCCGTTCCACTGTCTGGCCAAGCAGTTCTACCAGCTGTACCGGGAAAAA
GTGGACATATTCCATACCCTGGTGTGAGGGTACAGTGGACTGTCCTGAAGGGCTGCCCACTCTGCCGCTG
CTGCTGTGAGCCCAGCAGACCATTGTCTTTTCAGGAGACAGAAGACGGGATCACCTGGGATTTCTCAGTA
TCCACCATGGTTTTCAATAAAATGAGAAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4502942 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502942 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10732 same embryo
 EMAGE:10731 same embryo
 EMAGE:10730 same embryo
 EMAGE:10729 same embryo
 EurExpress:euxassay_003000 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS