EMAGE mouse gene expression spatial database

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EMAGE:10729

Pex19 peroxisomal biogenesis factor 19 ( MGI:1334458)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002992. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002992_01	euxassay_002992_02	euxassay_002992_03	euxassay_002992_04

euxassay_002992_05	euxassay_002992_06	euxassay_002992_07	euxassay_002992_08	euxassay_002992_09

euxassay_002992_10	euxassay_002992_11	euxassay_002992_12	euxassay_002992_13	euxassay_002992_14

euxassay_002992_15	euxassay_002992_16	euxassay_002992_17	euxassay_002992_18	euxassay_002992_19

euxassay_002992_20	euxassay_002992_21	euxassay_002992_22	euxassay_002992_23	euxassay_002992_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10729_wholemount_strong.wlz

10729_wholemount_moderate.wlz

10729_wholemount_weak.wlz

10729_wholemount_possible.wlz

10729_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10729_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4240
Entity Detected:	Pex19, peroxisomal biogenesis factor 19 ( MGI:1334458)
Sequence:	sense strand is shown >T4240 CAGTAAGATGGCGGCTGCTGAGGAAGGTTGCGGTGTTGGGGTCGAAGACGACCGGGAACTGGAGGAGCTT CTGGAAAGTGCTCTTGATGATTTCGACAAAGCCAAACCCTCCCCAGAACATGCTCCGACCATCTCGGCTC CCGACGCTTCAGGACCCCAGAAGAGAGCGCCAGGAGATACTGCCAAAGATGCTCTCTTCGCCTCCCAAGA GAAATTTTTCCAGGAACTGTTTGACAGTGAGCTGGCTTCCCAAGCTACTGCGGAGTTTGAGAAGGCAATG AAGGAGCTGGCTGAGGAAGAGCCCCATCTGGTGGAGCAGTTCCAGAAGCTCTCAGAGGCAGCTGGGAGAG TGGGCAGCGATGCAAGTTCTCAGCAAGAGTTTACTTCTTGCCTAAAGGAGACGTTAAGTGGCCTGGCCAA AAACGCCACTGAGCTGCAGAACTCGGGCATGTCTGAAGAGGAGCTGATGAAAGCCATGGAAGGGCTGGGC ATGGATGAGGGGGATGGGGAAGCGAGCATTCTCCCCATCATGCAGAGCATCATGCAGAACCTCCTGTCTA AGGATGTGCTGTACCCATCCCTGAAGGAGATCACAGAAAAGTATCCAGAATGGCTCCAGAGTCACCAGGA CTCCACTCCTCCCGAGCAGTTTGAGAAGTACCAGCAGCAGCACAGCGTCATGGTCAAAATCTGTGAGCAG TTTGAGGCCGAGACGCCCACAGACAGCGAGGCTACTCAGAGGGCTCGCTTTGAGGCCATGCTAGATCTCA TGCAGCAGTTACAGGCCTTGGGCCATCCTCCAAAAGAGCTGGCTGGGGAGATGCCTCCTGGCCTCAACTT TGACCTGGATGCTCTCAATCTGTCGGGCCCCCCAGGTGCTAATGGCGAACAGTGTCTGATCATGTGAAAC ACAGCACAGTTTCCTCCCTGAGCCCCAGCCGTGGGGAACATCTGGAGTCAACAGAGTCACTGGGAACTGA GGCAGGAGTATCATCTGTGGGAGCTGGCTGCCCCACCCACACTCTCCATCCCATTCAAGACTGTGCCATA CCAGCTGATCTGTAGGCCTTTTCTATGAGTCCTACTCCATCAGGGTTTCTGCTGTTAGAAACAGGCCAAT TATCTGCCAGGTGAAGGAAGGCATCCCTTGTGGGGCATTTCACTTCTTCCCACTCCCCAAATAATGTTAC ACATGGCAGTACTCATGTTCCCCTTGATTCCCAGGGACGTCGCGCCTTGTTTCTCCTCCCTCTCTGTGTC TGGGGAGGAGGAGCTGAGTCCTGACTCTGTGCTCCTGTTAGGCAGGGCCTCTGGGAAGAGGAGAAATGTA GCCTGAGCTGGGGCTGGAGGCTGGTCACCGCATATCCTTGCCTTCCTTTGGAATTCTCTTAAGGGAAAGG AATTTTGCACTTAGCCATGGTCGGGTTGGAAGCAAAGCCTTGGGCTTTTCCTGTCCCTAGGTGCTGAGCC CCAATCCCCCTTTCCTCCTGAGAACTCATGTTGCTGCCCTGGTTCTTTCTACAGCTCTGGGATTTAGGGG GAGCCAACTCTTGGCCTTCCTTTGCTCCCTGACTGTCCTTACGTCCTCTCTGTTTAGAGTCTTATTTATT CTGCCCTTTTAACCAGTGCTGTGATGGATGAAGGCCAATCTTGTTTCCTTTCATCTAGTACCTGGACCAT TCTCCCTAGACCCTTGCCTTCCTCCCTGGCCAACACATACTTGCAGTGTCAGATCTGGTACATGAATGGC CATTCTACCCAGATAGGTTTTAGCTTATATATAGGACATCAGATTCCCTGGAATCCTTGGTTCAGGAAGG TGCTTGGGAAAGGGGTGTAGCCAGAATATTTTTTCCCTGCAAGCCCTATGGGGTAGTATCTAATAAATAT TCTGAGCAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502819 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502819 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10732 same embryo
	EMAGE:10731 same embryo
	EMAGE:10730 same embryo
	EMAGE:10728 same embryo
	EurExpress:euxassay_002992 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source