EMAGE mouse gene expression spatial database

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EMAGE:10776

Slc16a6 solute carrier family 16 (monocarboxylic acid transporters), member 6 ( MGI:2144585)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001014. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001014_01	euxassay_001014_02	euxassay_001014_03	euxassay_001014_04

euxassay_001014_05	euxassay_001014_06	euxassay_001014_07	euxassay_001014_08	euxassay_001014_09

euxassay_001014_10	euxassay_001014_11	euxassay_001014_12	euxassay_001014_13	euxassay_001014_14

euxassay_001014_15	euxassay_001014_16	euxassay_001014_17	euxassay_001014_18	euxassay_001014_19

euxassay_001014_20	euxassay_001014_21	euxassay_001014_22	euxassay_001014_23	euxassay_001014_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10776_wholemount_strong.wlz

10776_wholemount_moderate.wlz

10776_wholemount_weak.wlz

10776_wholemount_possible.wlz

10776_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10776_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4293
Entity Detected:	Slc16a6, solute carrier family 16 (monocarboxylic acid transporters), member 6 ( MGI:2144585)
Sequence:	sense strand is shown >T4293 CCCACGCGTCCGGCGCGGGCGGCGGCACGCACGGCCCGGGAGGAGGCGACAGGGTTGGGGGGCGGGGGGA GGGTGTGGCTCGGGTGGCCGAGGCTGGCGTGGGTTGGGGGCCAGGGTACTCCAGGCTCCCCGCCCGGAGC CAACTTGATAGAGACTCTGGCCCACGCCCTAAGGGGTTAAACCTTTTTCTCATGTTACGGAGCGGTTTAT AAGAAGGCTCCGGAGGCCCAGCCTCCGCCTTATTGGGTGCCTTTTGGGGTTTATTCTCTTCCCTTCTAAC TTGATCAAGAATGACCCAAAAGAACTCGAAGCTTTGTGCCAGAGCCAATGTGTATACCCAAGTGCCTGAT GGAGGATGGGGCTGGGCAGTAGCTGTTTCTTTTTTCTTCGTTGAAGTCTTCACCTATGGCATCATTAAGT CATTTGGTGTCTTCTTCAATGACTTAATGGACAGCTTTGATGAATCCAATAGCAAGATCTCATGGATAAT ATCAATATGTGTATTTGTATTAACATTTACAGCTCCCCTCTCCACAGTCCTGAGCAACCGCTTCGGACAT CGGCTGGTGGTGATGGCAGGCGGGTTGCTCATCAGCTTGGGAATGATCACCGCCTCCTTCTCCCAAAGGG TCTACCACATGTACATCTCCATAGGGGTAATCTCGGGTTTAGGGTACTGCTTTAGTTTCCTCCCAACTGT GACCATTCTGTCGCAGTACTTTGACAAAAGGCGTTCGGTAGTCACCGCGGTCGCTTCTACGGGAGAGTGC TTCGCGGTGTTTGCTTTCGCACCAGCTATCACAGCTCTGAAGGAGCATATTGGCTGGAGATACAGCCTCC TCTTCGTGGGCCTTCTGCAGCTGAACATCATGGTCTGCGGCGCCTTGCTAAGACCAATTATTATCCAAGG GCCGGGGCAATCACCGAAAGCAGTCACTCTGGAGCCCCGCAGAGAAGTTCAGTACATGCTTGAAAACGAG AAAACACGGACCTCAATAGACTCCATCGACTCAGGAGTAGAACTAACTACCTCACCAAAAAATGTGCCTA GCGAAGCGAAGATGGAGCAGGAAACCAGGGCTGAGCAACAGCAGACCCTGGTGACTGCCCCCAAGCACAG CCAGATGAAAGCCCCGCTGTTGGACTTCTCCGTCTTGAAAGAGAAAAGCTTCATTTGTTACGCTTTGTTC GGCCTCTTCGCCACCCTGGGCTTCTTTGCGCCTTCCCTCTACATCATTCCTTTGGGTATCAGTCTAGGCA TTGACCCTGACCGCGCTGCGTTTTTGCTGTCGACGATGGCCATTGCGGAAGTGTTCGGAAGGATCGGGGC TGGGTTTGTCCTCAACAGGGAGCCCATTCGCAAGATCTACATCGAACTCATCTGCGTCATTTTACTGACG GCGTCTCTGTTCGCCTTCACTTTCGCTACCGAGTTCTGGGGCCTCATGCTGTGTAGCGTGTTCTTTGGCA GCATGGTTGGGACCATAGGAGGGACCCACATCCCGATGTTGGCAGAGGATGACGTCGTTGGGATCGAGAA GATGTCATCTGCAGCTGGAGTCTATGTCTTCATTCAGAGCATCTCGGGGCTTGCGGGACCACCCCTGGCA GGTCTGCTGGTTGACCAGAGCAAGATATACAGCAGGGCCTTCTACTCCTGCGCAGCCGGCATGTGCCTGG CTGCCGTGTGCCTCGCCCTGGTAAGACCCTGTAAAAAGGGCCTGTGCCAGAATTCTCATTCAGGTGAGAA CCAGACAGACCGGCAGCGTGGGAAGGCGTTACAGGACATACCCGAAGACTTTCTGGAAATGGATCTTGGG AAATGTGAACACAGGGCTCATATGAAAATGGATCCCGTGTGACACATTTTCACGTCGTGGCTTCTATGTC GCCTGGGAGCGGAGCCCCTGGGGATACAGGGAGGGGTAGGCGCTAACTACTAGACCCCCACCTTCAAACT TGCATGAAGGGAATGCCTGTCTGACAGCAGGGCCAGCAGCTGTCTCTTCCCCTCCAGTCCCTTTTGCTTG TTCTTTAAGCCAAAACCAAAACAACCAAACCTTTTCCATACAGGAATCCAGCCCTGTTCCGTAGCAATAC AAAGATAAGCGGAAGGACCTGGGGGGGTGGAGGCACCCCAGCACAAAAGCAATGCCACGAACTGACAGAC TGCTGTTACAAGCACTCCGTTCCTGTGATAAACTACTATATTATAAAGTGACATCCAGCCCAAGTCACAG AAATTAATTGGCCATTCCAAAACGACAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506255 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506255 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10778 same embryo
	EMAGE:10775 same embryo
	EMAGE:10777 same embryo
	EMAGE:10779 same embryo
	EurExpress:euxassay_001014 same experiment
	MGI:4828115 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source