EMAGE mouse gene expression spatial database

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EMAGE:10777

Zfp709 zinc finger protein 709 ( MGI:2384299)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002943. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002943_01	euxassay_002943_02	euxassay_002943_03	euxassay_002943_04

euxassay_002943_05	euxassay_002943_06	euxassay_002943_07	euxassay_002943_08	euxassay_002943_09

euxassay_002943_10	euxassay_002943_11	euxassay_002943_12	euxassay_002943_13	euxassay_002943_14

euxassay_002943_15	euxassay_002943_16	euxassay_002943_17	euxassay_002943_18	euxassay_002943_19

euxassay_002943_20	euxassay_002943_21	euxassay_002943_22	euxassay_002943_23	euxassay_002943_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10777_wholemount_strong.wlz

10777_wholemount_moderate.wlz

10777_wholemount_weak.wlz

10777_wholemount_possible.wlz

10777_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10777_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4299
Entity Detected:	Zfp709, zinc finger protein 709 ( MGI:2384299)
Sequence:	sense strand is shown >T4299 CCCGAGGTGACTGATCGATCCTCCGTGGCCCGGAGACGGGCGAGCCCAGGAACCGCCGCGGGGCCGCTGG AGGCCCTGGGAGCAGACATGGGCTCTGTGAGCTTTCAAGATGTGGCTGTAACCTTCACTGTGGAGGAATG GGCTTTACTAGATCCTTCCCAAAAGAAGCTGCACAAAGATGTAATGGAGGAGACCTTCAGGAACCTGGCT GCTATAGGAAAAACACAAGAAGGCCAGAACATAGATGAGGACCATAGATATTCTAGAAGAAATCAAAGAA ATGAAGTTGTGGAAAGACTGTTGGAGCATAGCAAAGGTACTGAAAGTAAAGAAATCTTCAGACAGATTCC AAACACCATTGTGAACAGGAAAAGTTCTCATAGAACAATGCTTAGTGAAAACCATGTGTACAAGGACAAC GTGCTCATTAGTCAGTCATCTCTTACTGTATCTGTGCCTCTTCAGACTCCACACAAACCTCATGAATATG AGCTACATGGAGAGAACCTCTATAAATTTGGAGAATGTGAGAAAGCCTTCATTTATCCAGAATGCTTTCT GAAGCATAAAGACAGTGGCACTAGACAGAGATCCTATGAGTTGAACCAATGTGAGGAAATCTTCAAAAGT AATGATTACATTCAAATACGTGTGAATGATGAAACTAGAGAAGAAATATGTATAGATAAGCAGCATTATG CCTTTAATTACCCTGATTTTCTTCAGTATGTTGAAAAGGTCCACACTGGAAAGAAACCCTATGCGTGTAA ACAATGTGGAAAAACCTTTTCTTTTTTGAGTAACATTAAAAGACATGAACGGATTCACTCTGGAGAGAAA CCATACAGGTGTAATATATGTGATAAAGCCTTCACTTGTTCAACTAACTTTCAAGAACATGAAAGAACTC ATACTGGAGAGAAACCCTATTTATGTACACAATGTGGTAAATCCTTTTCTTTTCTGAGTAACATTCGAAG ACATGAACGTACTCATACTGGAGAGAAACCGTACAGATGTAATGCCTGTGGTAAGGCTTTCAGTTATTTG ACAAACTTTCAAGACCATGAAAGAACTCATACTGGAGAAAAACCTTACGTGTGTACACAGTGTGGGAAAG CTTTCACTTACTACTATTCATTTCAGACACATAAACGATGTCACACTGGTGAAAAACCCTATGTATGTAA GCAATGTGGGAAAGCCTTCAGTTACTATAATTCCATTCAAACACATAAACGTTGTCACACCGGAGAAAAG CCCTATGTATGTAAGCTATGTGCTAAAGCCTTTACTACTCTCAGTTCTCTTCGATATCATGAGAGGATTC ACAGTGGTGAGAAGCCCCATGTATGTTTGCAATGCGGGAAAGGTTTCAATTCTTCCAGTACCCTTCGAAT ACATGAAAGGACTCACACTGGAGAGAAACCCTATAAATGTAAGAAGTGTGGAAAAGTCTTTAGTGTTGAC AGTTCCCTCCGATGCCATGAAAGGATTCACAGTGGGGAGAAGCCCTATGTATGTAAGCAATGTGGAAAAG CCTTCACTCGTCATACCTCCCTTCGATGCCATGAAAGGATTCACTGTGGGGAGAAACCCTATATATGTAA GCAATGTGGGAAAGGTTTTATTTCTTCCAGTAACTTTCGAAAACATGAATGGACTCATTCTGGAGAGAAA CTCTGTGTATGTAAACTATGTGGTAAAGCCTTCACTGGACATAGTTCACTTCAACGCCATGAAAGAATTC ACAGTGGAGAAAAACCCTATGTATGTAAGCATTGTGGGAAAGATTTCTCTTCTTTGAGTGGTTGTCAAAG ACATGAACAAATTCACACTGGTGAAAAACCCTATGTATGTAAGAATTGTGGGAAAGCCTTTACTAATCCC AGTGCCCTTCGAAATCATGAAAGGATTCACAGTGGTGAAAAACCCTATGTATGTGGACAGTGTGGGAAGG GTTTCATTTCTTTTAGTAAGTTTAGAATACATGAACGGATTCACACTGGAGAAAAACCATATAAGTGTAA GCAATGTGGGAAAGCCTTCACTGGTCACAGTTCCCTTCGACGTCATGAAAGGATTCACAGTGGAGAGAAA CCTTTCGTATGTAAACACTGTGGGAAATCCTTTTATACTTTAAGTGATTGTCAAAGACATGAACAGATCC ACACTGGTGAGAAACCTTTCATTTGCAAGCAATGTGGGAAAGCTTTTACCCGGTCCAGTTCCCTTAAAAT ACATGATAAGACTCACAGTAGAAGTCCCTAAGTATAGAACATGGAAAGGCATTCTTAGCATGACAATGCA TCGGGCAGAGACCCGGGGCCATGTTGTCTGTAGGAATGCCTCTAGTCATCTTGATGGCATTCACCTATGT GGTAGTTCATAGCATAGTGAGAAGCTCCAACATGATGTCAATAGAGACACATACCTTAAATGGATGTATA GAGAAGTCTTCAGTTGGCTAACCTCTGAGAAAGTACCCAATATCAAGTTGTATAACAAAATTATCAAAAC AGTTTACAAAGATTTATGAAAATTCCTTAAGTAATGAAATTCATTAATTCGTTTTAAGATCCCTGTAACT AAACACTAACTGGTCAATAATTCAGAAGATAAATCACTAGAATGTTTACTTATAATTTTGACTCTTGTAA GTAAATTTTCAGATGACCTAATCTCTAAGTGTCTTTAAGCAGTAAGGCATGAATTTAATGGGTTGTGTCT TTACAAGTCATGTACATTTCTTCTTTCTGTATTGGATTCAAATGAGATGTATTCCTAATATGTTTTAGAG TAATGTTAATGAGCTGTTGAAAAATGAAATTTTATTTTTAGGCTTTCTCTATTGACATCATGTGGGAACT TCTTATCTTTGATATTTTTGTATTTCTCTTGTTTGTTTTTTGTTGTTGTTGTTGGATTTTCATTTGAGTC AGGGTTTCTCTGTGTAGCCCTGGCTACCCTAGAGCTGGTTCCTTAGACCAGACTGGCATCAAACTTGGAG ATCTATCTGTCTCTGCTTCCCCAGTGCTGGGAATAAAGGTGTGTGCCATCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506799 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506799 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10778 same embryo
	EMAGE:10775 same embryo
	EMAGE:10776 same embryo
	EMAGE:10779 same embryo
	EurExpress:euxassay_002943 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source