EMAGE mouse gene expression spatial database

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EMAGE:10817

Dph2 DPH2 homolog (S. cerevisiae) ( MGI:1914978)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001242. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001242_01	euxassay_001242_02	euxassay_001242_03	euxassay_001242_04

euxassay_001242_05	euxassay_001242_06	euxassay_001242_07	euxassay_001242_08	euxassay_001242_09

euxassay_001242_10	euxassay_001242_11	euxassay_001242_12	euxassay_001242_13	euxassay_001242_14

euxassay_001242_15	euxassay_001242_16	euxassay_001242_17	euxassay_001242_18	euxassay_001242_19

euxassay_001242_20	euxassay_001242_21	euxassay_001242_22	euxassay_001242_23	euxassay_001242_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10817_wholemount_strong.wlz

10817_wholemount_moderate.wlz

10817_wholemount_weak.wlz

10817_wholemount_possible.wlz

10817_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10817_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

regional

moderate expression: see section 01 05 06 07 08 weak expression: see section 02 03 04 09 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4695
Entity Detected:	Dph2, DPH2 homolog (S. cerevisiae) ( MGI:1914978)
Sequence:	sense strand is shown >T4695 CCGGGAATTTGGAAGCGTACGGTTTGGATCCTAGGACCCGAGGACAGGTTGGTCCGGATCCACAAACAGA GCTGCTGTGACCTCGAGGACTTTTGAGGGTGCAGCTGCATCTCACCCCACCCAAGCTGCGCCCAATGGAG TCTACGTTCAGCAGCCCTGCGGAGGCAGCCCTGCAACGGGAGGCGGGCGTTCCAGGACAGTTCACTCCTC CTGAAGACCTGGACCGCGTGTATGAGCTGGAGCGAGTTACGAAATTTGTCTGCGATTTAGGTTGTCAGCG GGTGACTTTGCAGTTCCCTGACCAGTTACTAGGAGATGCGGGAGCGGTGGCTGCCCGGCTGGAGGAAGTC ACAGGAGCTAAGATGTTCATTTTAGGGGACACAGCTTATGGCAGCTGCTGTGTGGATGTGCTGGGCGCCG AGCAGGCTGGAGCTCAAGCCCTTGTGCACTTCGGTCCTGCCTGCTTAAGCCCCCCAGCCTCGCAGCTGCC CATCACCTTTGTCCTTGGTCAGCGACCCGTTGCTTTAGAGCTCTGTGCAAAGGCCTTTGAAGCCCAGAAC CCAGATCCGACAGCCCCGGTGGTACTGCTGAGTGAGCCAGCTTGTGCCCATGCCCTAGAGCCTTTGGCCA TGCTCCTGCTCCCAAAGTACCAAGATCTGCTCATCTCCCGCCCAGCTCTTCCCCTGCCAGTGGGATCCCC GAGCTCACAGCCTGAGTCCCTGGAGCGTTTTGGGCGCTGCTTCCCCCTGAATCCAGGGAGACGTCTGGAA GAATATGGTGCCTTCTATGTAGGGGCTTCTCAAGCAAGCTCGGACTCCAGCCTTGATCCCGACCTGAGCA GACTGCTCTTGGGTTGGACACCAGGGCGGCCCTTCTTTTCCTGTTGTCCAGATACAGGACAGACACAAGA CCAGGGTGCCAAGGCTGGGAGACTAAGAGCACGAAGACTGTATCTCATAGAGAGGGCCAGAGATGCCCGT GTGGTTGGGCTGCTGGCAGGCATATTAGGTGTGGCTCGACACCGTGAGGCACTGGCACACTTGCGGAAAC TGACGGAAGCTGCTGGAAAACGTAGCTATGTGTTAGCCGTGGGGAAGCCCACACCCGCCAAGCTTGCCAA CTTCCCTGAGATGGACGTCTTTGTGCTGTTAGCCTGTCCCCTGGGGGCACTAGCCCCCCAGCCTTCGGGT GGCTTCTTTCGGCCTGTATTGACGCCATGTGAATTGGAGGCTGCCTGCAACCCTGCCTGGCCCCCGCCAG GCCTGGCTCCCCACCTCACACATTATGCAGAGCTGTTGCCTGGTTCTCCCTTCCATGTGCCACTCCCTCC ACCTGAGTCAGAGTTGTGGGATACCCCAGATGTGTCACTCATTTCTGGGGAGCTCCGACCACCACCTCCT TGGAAGTCATCAGATGACACTAGATGTTCGGCCTTAATTCCGAGGCCCCAACTGGAGCTGGCGGAGAGCA GCCCTGCAGCTTCATTCCTTAGTTCTCGGAACTGGCAGGGGCTGGAGCCACGCTTGGGCCAGACACCAGT GAAAGAAGCCGTCAGAGGAAGACGAGGTATCGCCATCGCCTACGAGGATGAGGGGAGCAGCTGATACCTT GGGAGGCCAGAGCCAGAGTGATAAGTCTGCCGGGACCTGTTCTCCCTGACCAACGTCTCGTCCTGTCACA GGGACACTTGATGCCTAGACACAGGGAGTGTAGGTAGCTCTCTCTGTGGAGCTGTCCAGCTTATAGCCTG TGCAGGGAGGGCTCCCAGGGCTCATCCCAGTTCCTGGTGTGCAGGAGTTCTGCCCTTGGTCAGGGGCAGA AGTTGGATGATCCAGACCAGAGTTCTTCCACCTCCCTCAGCACTGTTGACAGTTTGGGCAGAGTAATTCT TTAATGCAGAGGCTACTCTATATAGAGAAGGATGTATAGGTTCAGCTGACAGAAACAGAAACACCTCCCT TCCTCCCCAACTGAGCTAGGATAACCCAGATTATCTCTACAGGTTGGCAGATGTCATGTAACTACTCCTC ATTGAGAGCCTCTGGTCTAAACCTGCACTGTCCAAGACAATATTCACTGGACACATGACTTTTGCCATTT AAAGTGGATGAAAAAGTATTTGTCCATTACACTAATTACATTTCAAGCACTTATGTGGCTGCTTTCAGGA CTTTGTTGGGCTACATTGACTTAGTTAGACTTGTTTCTTGGGGACAACAAAGACACTGAAATAAACGGAA TACTTTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5029876 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5029876 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10815 same embryo
	EMAGE:10816 same embryo
	EMAGE:10814 same embryo
	EMAGE:10813 same embryo
	EurExpress:euxassay_001242 same experiment
	MGI:4824380 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source