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EMAGE:10842

Tomm6 translocase of outer mitochondrial membrane 6 homolog (yeast) ( MGI:1913369)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842
"Pseudo-wholemount" of euxassay_001208. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001208_01 euxassay_001208_02 euxassay_001208_03 euxassay_001208_04
EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842
euxassay_001208_05 euxassay_001208_06 euxassay_001208_07 euxassay_001208_08 euxassay_001208_09
EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842
euxassay_001208_10 euxassay_001208_11 euxassay_001208_12 euxassay_001208_13 euxassay_001208_14
EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842
euxassay_001208_15 euxassay_001208_16 euxassay_001208_17 euxassay_001208_18 euxassay_001208_19
EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842 EMAGE:10842
euxassay_001208_20 euxassay_001208_21 euxassay_001208_22 euxassay_001208_23 euxassay_001208_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10842Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10842_wholemount_strong.wlz
10842_wholemount_moderate.wlz
10842_wholemount_weak.wlz
10842_wholemount_possible.wlz
10842_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10842_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4867
Entity Detected:Tomm6, translocase of outer mitochondrial membrane 6 homolog (yeast) ( MGI:1913369)
Sequence:sense strand is shown

>T4867
ATTGGTACTGCACGCTCTCTGACCTGAAAGACACTTTCTTGGGAGCATCCCACTGCCCAGCGCCTCCAGG
GTGAGCTAACCCCTATCCGCAGAGGCTGGGCTAGCCTCACCGTCATCACCTTGCTTAAACTAACTGGGTT
GTAGAGCAGCACTGGGAACCGACCCCCGTCCCTGGGAGGCGGGGCCGCGAGCTGGGGCTCCGCCTCCGAT
CAGGCGTCCTGGCTCTACGAACTCCTGGAGGCGGTGGGGGCGGGCCTTCTCGCTCATTTCTTACGCTGAT
CGGCGGAAGTGCGGAGATCGGAAGTTGCGCGATCGGCTTGCGACGACCCGGTGCGACGCTCGGTTGGTAC
GTGCTTAGTGCTGGTATGGCTTCCAGCGGAGTGACTGTGAGCGCCGCCGGGTCGGCCAGCGAGGCCTCAG
AGGTTCCAGACAACGTGGGAGACTGGCTCCGCGGCGTCTTCCGCTTCGCCACCGATCGAAACGACTTCCG
GAGGAACTTGATCCTCAATTTGGGACTCTTTGCTGCGGGAGTCTGGCTGGCCAGGAACTTGAGTGACATT
GATTTGATGGCCCCTCAGCCAGGGGTGTAGCCAGGTAAGACTTCACTTTCCCCTGCCTATTTCAGGAATC
CTAAAAGATGAAGACAGCAGTATGTTCTGTCAGCTAGTCACAGAAGTAGGGAAGATTCTTGGGGTAAAGC
CTTGCCCTTGTGTTTCTAGGTGAGAGCAGCTCTCCTGTGTTTCCCCGTTTCTGATGCTGTTATCTGCTTA
CAGAGAATGGAACTCCTGTGTATTCAGACTTTCCAAAGACAGCCTACTGTCTGTGACCACAAGATCCTAC
CTGAGTGGCAGCTGAAGTTGACTCCCTCTCCTTGCCTGAACCCCCCCCCCCACTGCCCCCCCATCCCCCA
GTGTCGGCTGAGATGTTGCCTCTGCACGGTTCTGTGTGCAGTTCCCAACTTTCTGCAGAAGATGGTCCTT
GCCCTTGTCCTGAAGAGTAGTAATGGTTCTTGAAAAAGATTTCAAATAAAGCCTGCACATAAAAAAAAAA
AAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5068609 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5068609 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10841 same embryo
 EMAGE:10845 same embryo
 EMAGE:10844 same embryo
 EMAGE:10840 same embryo
 EMAGE:10843 same embryo
 EurExpress:euxassay_001208 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS