EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10845

Rprd1a regulation of nuclear pre-mRNA domain containing 1A ( MGI:2385066)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001211. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001211_01	euxassay_001211_02	euxassay_001211_03	euxassay_001211_04

euxassay_001211_05	euxassay_001211_06	euxassay_001211_07	euxassay_001211_08	euxassay_001211_09

euxassay_001211_10	euxassay_001211_11	euxassay_001211_12	euxassay_001211_13	euxassay_001211_14

euxassay_001211_15	euxassay_001211_16	euxassay_001211_17	euxassay_001211_18	euxassay_001211_19

euxassay_001211_20	euxassay_001211_21	euxassay_001211_22	euxassay_001211_23	euxassay_001211_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10845_wholemount_strong.wlz

10845_wholemount_moderate.wlz

10845_wholemount_weak.wlz

10845_wholemount_possible.wlz

10845_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10845_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 20 21 22

dorsal root ganglion

weak

regional

weak expression: see section 11 13 18 19 20

kidney calyx

weak

regional

weak expression: see section 09 10 11 12 13 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4864
Entity Detected:	Rprd1a, regulation of nuclear pre-mRNA domain containing 1A ( MGI:2385066)
Sequence:	sense strand is shown >T4864 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGATTCTCCTCCCCTCGGCTCCACGGCGTAGGACGGC GCGAACGTGGTTCCGGAGGGATGTCCGCCTTCTCTGAGGCGGCGCTGGAGAAGAAGCTGTCCGAGTTGAG CAACTCGCAGCAGAGCGTGCAGACCCTGTCCCTGTGGCTCATCCACCATCGCAAACACTCGCGGCCCATC GTCACGGTGTGGGAACGGGAGCTACGCAAAGCCAAACCCAACAGGAAGCTTACTTTTCTCTACTTGGCCA ATGATGTCATTCAAAACAGCAAAAGGAAGGGGCCAGAGTTCACAAAAGATTTTGCACCAGTAATAGTGGA GGCATTCAAGCATGTTTCAAGTGAAACTGATGAAAGCTGTAAGAAGCACCTTGGAAGAGTGTTATCTATT TGGGAAGAACGATCTGTTTATGAAAATGATGTATTAGAGCAACTCAAGCACGCGCTGTATGGAGATAAGA AGGCAAGGAAGAGGACTTATGAGCAAATAAAGGTGGATGAGAATGAAAATTGTTCCTCTTTAGGGTCCCC AAGTGAGCCACCACAGACTCTCGACCTTGTTAGAGCATTACAAGATTTAGAAAATGCAGCCTCAGGTGAC GCAGCAGTCCATCAAAGGATAGCGTCTTTGCCTGTCGAAGTTCAAGAAGTGTCCCTGCTTGAGAAGATAA CAGACAAAGAATCTGGAGAGAGGCTTTCTAAAATGGTAGAAGATGCTTGTATGCTGCTGGCAGATTATAA TGGCAGACTGGCGGCAGAAATAGATGATAGAAAGCAACTCACTCGAATGTTAGCAGATTTTCTTCGTTGT CAAAAGGAAGCCCTTGCAGAGAAAGAACATAAATTGGAAGAATACAAGCGCAAGTTAGCCCGAGTTTCCC TGGTACGCAAAGAACTAAGAGCCCGGATCCAGAGCCTGCCAGATTTATCTCGTTTGCCCAATGTCACTGG CAGTCACATGCACCTGCCCTTTGCGGGGGACATCTACAGTGAAGACTGATGGCCAGTCTCCTCCAGGGCC AGGACTTTGGCAGACATGGAGACAGGTTAGGTGAATAGTCCTGTAGAGTTATTTTTGTACATTGTTGAGA ATGGTACTAACACAAGAAATGACAAGGAAGTATAAAATGTTAAAATGTTGGTTTGTTTTGCTATTTTATT AGTGAGTTAACATGACTTAGTTAAAACCAAGTGATGATTTCTGTGTATTAATGAACTTAATGATTATTTT CAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5067723 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5067723 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10841 same embryo
	EMAGE:10842 same embryo
	EMAGE:10844 same embryo
	EMAGE:10840 same embryo
	EMAGE:10843 same embryo
	EurExpress:euxassay_001211 same experiment
	MGI:4827805 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source