EMAGE mouse gene expression spatial database

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EMAGE:10955

Acp5 acid phosphatase 5, tartrate resistant ( MGI:87883)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003591. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003591_01	euxassay_003591_02	euxassay_003591_03	euxassay_003591_04

euxassay_003591_05	euxassay_003591_06	euxassay_003591_07	euxassay_003591_08	euxassay_003591_09

euxassay_003591_10	euxassay_003591_11	euxassay_003591_12	euxassay_003591_13	euxassay_003591_14

euxassay_003591_15	euxassay_003591_16	euxassay_003591_17	euxassay_003591_18	euxassay_003591_19

euxassay_003591_20	euxassay_003591_21	euxassay_003591_22	euxassay_003591_23	euxassay_003591_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10955_wholemount_strong.wlz

10955_wholemount_moderate.wlz

10955_wholemount_weak.wlz

10955_wholemount_possible.wlz

10955_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10955_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

single cell

strong expression: see section 05 06 07 08 09 22 23 24

humerus

strong

single cell

strong expression: see section 01 02 03 04

femur

strong

single cell

strong expression: see section 03 04 05 22 23

meckel's cartilage

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24

palatal shelf

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

clavicle

strong

single cell

strong expression: see section 08 09 10 11 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6607
Entity Detected:	Acp5, acid phosphatase 5, tartrate resistant ( MGI:87883)
Sequence:	sense strand is shown >T6607 CCACGCGTCCGCGGACGCGTGGGTCCGGAGCGACCCGCCGCGAATCCGCAGCTCAGTTGGGTAGCACAGC TTGTCCTGGACCCACCGCCAAGATGGATTCATGGGTGGTGCTGCTGGGCCTACAAATCATATGGCTCCCA CTCCTGACCCACGGTACAGCCCCCACTCCCACCCTGAGATTTGTGGCTGTGGGCGACTGGGGAGGGGTCC CCAATGCCCCATTCCACACAGCCCGGGAAATGGCCAATGCCAAAGAGATCGCCAGAACCGTGCAGACGAT GGGCGCTGACTTCATCATGTCTCTGGGGGACAATTTCTACTTCACTGGAGTGCACGATGCCAGCGACAAG AGGTTCCAGGAGACCTTTGAGGACGTGTTCTCTGACCGTGCCCTTCGCAACATCCCCTGGTATGTGCTGG CTGGAAACCATGATCACCTTGGCAACGTCTCTGCACAGATTGCATACTCTAAGATCTCCAAGCGCTGGAA CTTCCCCAGCCCTTACTACCGTTTGCGCTTCAAAATTCCACGTACAAACATAACTGTGGCCATCTTTATG CTGGACACAGTGATGCTGTGTGGCAACTCAGATGACTTTGCCAGTCAGCAGCCCAAAATGCCTCGAGACC TGGGAGTGGCCCGCACTCAGCTGTCCTGGCTCAAAAAGCAGTTGGCAGCAGCCAAGGAGGACTACGTTTT GGTGGCCGGCCACTACCCCATCTGGTCCATCGCCGAGCACGGACCCACGCGCTGCCTTGTCAAGAACTTG CGACCATTGTTAGCCACATACGGGGTCACTGCCTACCTGTGTGGACATGACCACAACCTGCAGTATCTTC AGGACGAGAACGGTGTGGGCTATGTGCTGAGTGGGGCCGGCAACTTCATGGATCCCTCTGTGCGACATCA ACGAAAGGTCCCCAACGGCTACTTGCGGTTTCACTATGGATCTGAGGACTCCCTGGGCGGCTTCACACAT GTGGAGATAAGCCCCAAAGAAATGACCATCATATATGTGGAAGCCTCTGGAAAATCACTCTTTAAGACCA GCCTCCCAAGGAGACCCAGACCCTGAACACCACGAGAGTCCTGCTTGTCCCCTAACGGGTGTGAGGGAGG AGGCGTCTGCAGGCGTGGTGGGTGGGCCCTGTGAGGACCTCACTCACGGCAGCTTCTCTGCCCTGGTACT CTGGCAGAGAGGGAAGGGGAACCAAGGGGCTTACTTACAGGAAAGAAGTATGCCCACACCAACTGATCCC ACAGCCAGGCTCACCCCTTTTAGGGTAGAGCTTTCTTGGGAACGGGCTTTCCTTTCAGTCAAGTCTTACT GTCACTGCTATTCAATAAAACAATGCTTTGTCTCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3596147 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3596147 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10953 same embryo
	EMAGE:10956 same embryo
	EMAGE:10957 same embryo
	EMAGE:10952 same embryo
	EMAGE:10954 same embryo
	EurExpress:euxassay_003591 same experiment
	MGI:4822934 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source