EMAGE mouse gene expression spatial database

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EMAGE:10956

Pnp purine-nucleoside phosphorylase ( MGI:97365)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003605. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003605_01	euxassay_003605_02	euxassay_003605_03	euxassay_003605_04

euxassay_003605_05	euxassay_003605_06	euxassay_003605_07	euxassay_003605_08	euxassay_003605_09

euxassay_003605_10	euxassay_003605_11	euxassay_003605_12	euxassay_003605_13	euxassay_003605_14

euxassay_003605_15	euxassay_003605_16	euxassay_003605_17	euxassay_003605_18	euxassay_003605_19

euxassay_003605_20	euxassay_003605_21	euxassay_003605_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10956_wholemount_strong.wlz

10956_wholemount_moderate.wlz

10956_wholemount_weak.wlz

10956_wholemount_possible.wlz

10956_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10956_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 12 13 14 15 16

thyroid gland

strong

regional

strong expression: see section 15 16 moderate expression: see section 12

vibrissa

strong

regional

strong expression: see section 04 05 06 07 19 20 21 22 moderate expression: see section 03 18

retina

strong

regional

strong expression: see section 01 02 03 04 21 22

anterior naris

strong

spotted

strong expression: see section 11 12 13 14

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

liver right lobe

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21 22

renal cortex

strong

regional

strong expression: see section 08 09 10 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6651
Entity Detected:	Pnp, purine-nucleoside phosphorylase ( MGI:97365)
Sequence:	sense strand is shown >T6651 CGGACGCGTGGGGCGGAAAGGGCAGGATTTCGGCGTGGCCGTCGGTGACTCCATGGAGAACGAGTTCACA TACGAAGATTATGAGACCACTGCCAAGTGGCTTCTGCAACACACTGAATATCGACCTCAAGTGGCAGTGA TCTGTGGTTCCGGCTTAGGAGGGCTGACTGCTCACTTAAAGGAGGCTCAGATCTTTGACTACAATGAGAT ACCCAACTTTCCCCAAAGCACAGTGCAAGGTCACGCAGGCCGACTGGTGTTTGGATTGCTGAATGGCAGA TGCTGTGTGATGATGCAAGGCCGGTTCCATATGTATGAAGGATACTCACTGTCAAAGGTGACATTCCCAG TGAGAGTTTTCCATCTTCTGGGTGTGGAAACTTTGGTGGTCACCAATGCTGCTGGAGGACTCAACCCTAA TTTTGAAGTTGGAGATATTATGCTGATCCGTGATCACATCAACCTACCTGGTTTCTGTGGCCAGAACCCT CTCCGGGGCCCCAACGATGAAAGGTTTGGAGTTCGTTTTCCTGCCATGTCTGATGCTTATGACCGGGATA TGAGGCAGAAGGCTTTCACTGCCTGGAAACAAATGGGGGAGCAACGAAAGCTACAAGAAGGCACCTACGT GATGTTGGCAGGCCCCAACTTTGAGACTGTGGCAGAGAGTCGTCTGCTAAAGATGCTGGGAGCAGATGCT GTTGGCATGAGCACAGTCCCAGAAGTTATCGTCGCAAGGCACTGTGGGCTTCGAGTCTTTGGTTTCTCAC TCATTACGAACAAGGTTGTCATGGATTATGAGAACTTGGAGAAGGCCAATCACATGGAAGTTCTGGATGC CGGGAAAGCAGCTGCACAGACATTGGAAAGGTTTGTCTCCATTCTTATGGAGAGCATTCCACTCCCGGAT CGTGGCAGCTGACCTGCAATGGAGTGCTCCGCTGCTCCCCTGGTGGGGTCCAAGTAGCTCTCTACTCTGG TTCCTTGATGAAGTCACTAGTCTCTGTTCTTAGATCCTAGCAGAAAGGAAAGAGGAGCTTACCCTTCACT TTCCAGATGTTGTTCTACTGTTCTAATGCCCAGTCTTCTTGCTTAGTTGCCTTCTCAAATTAACTTTCAT TCCTCTGGAAGAGCTGGAGCCCATTCCTCCCACACTTGAGATATATGCCTTCAAACAGTACCTTCTACAT CTTGGAGATGACAGACATGTTCTGGGCTCAGTCTAAAGCCATAAGGACCAAAGATCAACCCAATACCTCT TGGATTTTATTTAGTATCACAATGTTTTGAGAATAAAGAGATCGAGTACTTTTCAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3600212 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600212 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10955 same embryo
	EMAGE:10953 same embryo
	EMAGE:10957 same embryo
	EMAGE:10952 same embryo
	EMAGE:10954 same embryo
	EurExpress:euxassay_003605 same experiment
	MGI:4827303 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source