EMAGE mouse gene expression spatial database

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EMAGE:11008

Arxes1 adipocyte-related X-chromosome expressed sequence 1 ( MGI:1923469)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001122. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001122_01	euxassay_001122_02	euxassay_001122_03	euxassay_001122_04

euxassay_001122_05	euxassay_001122_06	euxassay_001122_07	euxassay_001122_08	euxassay_001122_09

euxassay_001122_10	euxassay_001122_11	euxassay_001122_12	euxassay_001122_13	euxassay_001122_14

euxassay_001122_15	euxassay_001122_16	euxassay_001122_17	euxassay_001122_18	euxassay_001122_19

euxassay_001122_20	euxassay_001122_21	euxassay_001122_22	euxassay_001122_23	euxassay_001122_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11008_wholemount_strong.wlz

11008_wholemount_moderate.wlz

11008_wholemount_weak.wlz

11008_wholemount_possible.wlz

11008_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11008_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 05 06 07 08 21 22 23

hypothalamus mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 15 16 17 18 19

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 weak expression: see section 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

weak

homogeneous

weak expression: see section 12 13 14 17 18 19 20

hindbrain

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

weak

homogeneous

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 weak expression: see section 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 weak expression: see section 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 08 09

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 20

spinal cord mantle layer

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 weak expression: see section 17 18

cornea stroma

weak

regional

weak expression: see section 02 03 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4229
Entity Detected:	Arxes1, adipocyte-related X-chromosome expressed sequence 1 ( MGI:1923469)
Sequence:	sense strand is shown >T4229 GGCAGAGGGACCGCGGAGCGAACTGCACGCCCCGTGCCGAGCCAGCCAGCCAGCCAGCCAGCCAGCGAGC GCCGGTGGAGCCCGCGTGGAGGGAGCCGCCGATTGAACACCCTGCAAGATGAACTCCCTGCTGTCCCGGG CCAACTCGCTGTTCGCCTTCACGCTGAGCGTCATGGCGGCGCTCACCTTGGGCTGCATCCTCACCACCGC CTTCAAAGACAGGAGCGCGCCCGTGCGCCTGCACGTCTCCAGGATCCTGCTCAAAAAAGTGGAAGACTTC ACCGGACCCAGAAAGAAGAGCGACCTGGGCTTCATCACCTTCCACATCTCTGCGGATCTGGAGAAGACTT TCGACTGGAACGTCAAGCAGCTCTTCCTTTATCTGTCGGCAGAATATTCCACCAAAAGCAACGCTGTGAA CCAGGTGGTCCTCTGGGACAAGATCCTTCTGCGAGGCGAGAACCCCAAGCTGAACCTGAAAGACGTCAAA AGCAAGTATTTTTTCTTTGACGATGGACACGGGCTCAAGGGAAACAGGAATGTCACTTTGACTCTCTCCT GGCAAGTGATACCGATTGCTGGGATCCTGCCTCTTGTGACTGGATCTGGACGCGTGTCTGTCCCGTTTCC AGATTCCTATGAAATAGCCACGACTTTTTGAAGAATTCTCTCTGCATCGGAAACGGGACATCTGTGGACC TAAGGAATCATAGTTTAAAACTGTCAAGAAACACGTGCTGTAACAATTTATGTGTCGCATTCCTGCATGC CTACACTGAATGTGATTTAGAAGCAGACGTTTGTTCACCTCGTGTAAAGCATGCTTGATTCCTTGGGGGG GGGGGGACAGTTAATATCGTTTGTTAGATACCATAAGTGGTGGAAATAAAGTGACTAAAGACCGAGTACA TGATGATTCTGTAAATCACTGTAACACATTATTGATATGCCGACTGTCTGAGAAATATGGGTCTTTCCAG GTTTTTCCTCCAAAAGTTTTTCTGTTTTACATTTTTTTGAAAAAAATTAATGTTTTAACCATTGTTAGTG AAGGTTGAAAACGTTTGCATGACTTTCCCATTGACTTTCAGTCTGTATGCTTACTACCACTAGGTGCTAA TTATGTACAGTTCTCCACAAGTCTCCATTTTAATAAGTTTTGGAGTAATATTAACACTCATCGTTTAATT TTTTTCATTTACAAACTCTCTTATATGTTATTGTATCCTATTGCCAGCAGTCTCGGGATGTATATAATAG AAACATGGGCTGTTTGCAGATGAACACTGTGAACCCCTGAAGCTTGGGGAATCATTTCAAGCTTAAATAG CTAGCAAAAAAAGTGGAGTCTAACTTTGATCTTGGATATTCTCATTCCCTAAGTTTATTCCACCCCTGAT ACCTGAGATGACATCATAGGAATTTCATGTGTAACTTACATTGTATTTTTGTAACCCTGAAACTCTCTGT TTTGATGTTTCTGATCTTATGTAAAAGTAGCTTGTGTTCAAGTTGTGTTAAAAAAAAACTTCTATAGGAA TAGATCTCCTTTCACACTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502106 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502106 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11009 same embryo
	EMAGE:11012 same embryo
	EMAGE:11011 same embryo
	EMAGE:11010 same embryo
	EMAGE:11007 same embryo
	EurExpress:euxassay_001122 same experiment
	MGI:4822786 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source