EMAGE mouse gene expression spatial database

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EMAGE:11011

Trim32 tripartite motif-containing 32 ( MGI:1917057)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002985. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002985_01	euxassay_002985_02	euxassay_002985_03	euxassay_002985_04

euxassay_002985_05	euxassay_002985_06	euxassay_002985_07	euxassay_002985_08	euxassay_002985_09

euxassay_002985_10	euxassay_002985_11	euxassay_002985_12	euxassay_002985_13	euxassay_002985_14

euxassay_002985_15	euxassay_002985_16	euxassay_002985_17	euxassay_002985_18	euxassay_002985_19

euxassay_002985_20	euxassay_002985_21	euxassay_002985_22	euxassay_002985_23	euxassay_002985_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11011_wholemount_strong.wlz

11011_wholemount_moderate.wlz

11011_wholemount_weak.wlz

11011_wholemount_possible.wlz

11011_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11011_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4230
Entity Detected:	Trim32, tripartite motif-containing 32 ( MGI:1917057)
Sequence:	sense strand is shown >T4230 CTCGCGTCGGAGCTGCGGGCGGTCAGGAATCTGACACTGGGGCATGAATATTAAGCTGTGTGGCTCTAAG CTGTGTTAGCAGGAACCTTCACTGGAAGAGCAATGGCTGCGGCTGCAGCAGCTTCTCACCTGAACCTGGA TGCCCTCCGGGAAGTGCTAGAATGTCCCATCTGCATGGAGTCCTTCACTGAAGAGCAGCTGCGACCCAAG CTGCTGCACTGTGGCCATACCATCTGCCGCCAGTGTCTGGAGAAGCTCCTGGCCAGCAGCATCAATGGCG TCCGCTGCCCCTTTTGCAGCAAGATTACTCGCATCACCAGCCTGACCCAGCTGACCGACAACCTGACGGT GCTGAAGATCATTGACACAGCTGGGCTCAGTGAGGCCGTCGGCCTGCTCATGTGCCGAGGCTGTGGCCGG CGGCTGCCTCGGCAGTTCTGCCGAAGCTGTGGTGTGGTGTTGTGTGAACCCTGCCGGGAGGCAGATCACC AACCCCCTGGCCACTGCACACTTCCGGTCAAGGAGGCAGCTGAGGAGCGGCGGAGGGACTTCGGGGAGAA GTTGACTCGTCTAAGGGAACTTACTGGAGAGCTGCAGAGGAGGAAGGCAGCCTTGGAGGGCGTCTCCAGG GATCTTCAGGCAAGGTATAAGGCTGTTCTTCAAGAATATGGCCATGAGGAACGCCGCATCCAGGAAGAGC TAGCCCGCTCTCGGAAGTTCTTCACAGGCTCCTTGGCTGAGGTTGAGAAGTCCAACAGTCAAGTGGTAGA GGAGCAGAGCTACCTACTCAACATTGCTGAGGTGCAGGCCGTGTCTCGCTGTGACTACTTTCTAGCGAAG ATCAAGCAAGCTGATGTAGCCCTCCTGGAGGAGACAGCGGATGAGGAGGAGCCCGAGCTCACTGCCAGCC TACCCCGGGAGCTTACCCTGCAAGATGTGGAGCTCCTTAAGGTAGGACACGTTGGTCCTCTGCAAATTGG CCAGGCTGTTAAGAAGCCCCGGACAGTTAACATGGAAGATTCCTGGGCAGGGGAGGAGGGAGCAGCATCT TCTGCCTCAGCCTCGGTAACCTTTAGAGAGATGGACATGAGCCCTGAGGAAGTAGCTCCCAGCCCTAGGG CTTCCCCCGCGAAACAGCGGAGTTCTGAGGCAGCTTCCGGTATCCAGCAGTGTCTGTTTCTCAAGAAGAT GGGGGCGAAAGGCAGCACTCCCGGCATGTTCAATCTTCCAGTCAGTCTCTATGTGACCAGTCAGAGTGAG GTGCTGGTTGCCGACCGGGGCAACTATCGTATCCAAGTGTTCAACCGCAAAGGCTTTTTGAAGGAGATCC GCCGCAGCCCCAGCGGCATTGATAGCTTCGTGCTAAGCTTCCTTGGAGCCGACTTGCCCAATCTCACTCC ACTTTCAGTGGCCATGAACTGCCATGGGCTGATTGGTGTCACTGATAGCTACGACAACTCCCTTAAAGTC TATACCATGGATGGCCACTGTGTGGCCTGTCACAGGAGCCAGCTGAGCAAACCATGGGGCATCACAGCCC TACCATCTGGCCAGTTTGTGGTGACTGACGTGGAAGGCGGGAAGCTCTGGTGTTTCACTGTAGACCGAGG AGCAGGAGTGGTCAAATACAGCTGCCTCTGCAGTGCTGTGAGGCCCAAGTTTGTCACCTGTGATGCTGAA GGCACAGTCTACTTCACCCAAGGCTTGGGGCTCAATGTGGAAAACCGACAGAATGAACACCACCTGGAGG GTGGCTTCTCCATCGGTTCTGTTGGCCCCGATGGGCAGCTGGGCCGGCAAATCAGCCACTTCTTCTCTGA GAATGAAGATTTCCGCTGCATCGCTGGCATGTGTGTAGATGCTCGGGGCGACCTCATTGTGGCAGATAGC AGCCGCAAGGAAATTCTCCATTTTCCCAAGGGCGGTGGCTACAGCGTCCTTATTCGAGAGGGCCTTACCT GTCCAGTGGGCATCGCCCTCACACCCAAGGGGCAGCTGCTGGTCTTGGACTGTTGGGATCACTGCGTCAA GATCTACAGCTATCATCTGAGAAGATATTCCACCCCTTAAAAGATGAGGAGAAATACCACTTTCTTCTTT CCCTCAATAGAGTTTCCTTCCCTTTCCTTCCTTCGTTGTTGATGGTGCCGTAGAGTAGATGTGGCCTGTG GCCTGATTCTAGTAGGGTTGCTCTAGAATCGTAGAAGCTCTAGTTTTCAGTGATTTTGTTTGTTATGCCC CTCTTCCCCCCCCCCTCCCCTTTTCTCCTTCTAACTTTAGAGCTTAGAGCTTTACCAGGTACCTTATCCC AAGTAGAATAGATGATGCTCTTAGCAGAAGTTGCTTAGAAGTTAGGTACTTCCAAGGCTTTAGTAAAGAG AACCACTTTAGTCCTTTGCCCTTGTGTTTGAAATTTGTATTGGTATTGAATAGTCATTGATATTTCCCTT TGGACTATGAGGTCCCTAGTCTACTGTCTCCTTCCTGTTACAGTGTGCTTCGTCTGTGGCTTTAAATCTG GCTGCATTCTGTGTGCATGCTCCAGTGGGATCTGCTTGACCCTTCAGTGATATTATATTCTGTAGCATGA TGTCTCCTTTGATCACCTTTACCAGTGTGAGAGTATATCATTAAGTGCCACCAAAGGTGACACATAGTCC CTTTGAAAGCAGGACCTCTTGACGGGAATTTCTGCCACCATAGATTGTCTTCTCATTGAAGATGTGCCAG AGGATGGTTTGACTGACCCTAGTTGGATAGTGTTGAACACTTCAGTCCAGAAATCACTTGCCTTTTAAAG GAGTTTATAGGTTAGAATTATGCCATGGCCAAGGCAGACGGGAACATATAAGCAGATGCTAACATATCCA GCAAATGTGGGATATTTTCTAGGGCTCAAAGCGTTTTGTACTCCTTCCACTGACCTATGCTAAGAGTTAT TGAAAGAATTGTGTAATGTGAGCTTCGACCAACAATGTGAGGAGGTTGACACATCCATTTTCATTACTAA AGAACAAACTGTTAGGTAGCTAGCTCTGTATTTTCTTCAAGTGAGTGCATATTGTTTGACTTTGAGATTT AGCTCTGTTCTATCAAGCATTAAACTAATAAAAGTAATAACTTTCTTCATCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502346 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502346 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11009 same embryo
	EMAGE:11008 same embryo
	EMAGE:11012 same embryo
	EMAGE:11010 same embryo
	EMAGE:11007 same embryo
	EurExpress:euxassay_002985 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source