EMAGE mouse gene expression spatial database

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EMAGE:11010

Supt7l suppressor of Ty 7 (S. cerevisiae)-like ( MGI:1919445)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003003. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003003_01	euxassay_003003_02	euxassay_003003_03	euxassay_003003_04

euxassay_003003_05	euxassay_003003_06	euxassay_003003_07	euxassay_003003_08	euxassay_003003_09

euxassay_003003_10	euxassay_003003_11	euxassay_003003_12	euxassay_003003_13	euxassay_003003_14

euxassay_003003_15	euxassay_003003_16	euxassay_003003_17	euxassay_003003_18	euxassay_003003_19

euxassay_003003_20	euxassay_003003_21	euxassay_003003_22	euxassay_003003_23	euxassay_003003_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11010_wholemount_strong.wlz

11010_wholemount_moderate.wlz

11010_wholemount_weak.wlz

11010_wholemount_possible.wlz

11010_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11010_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4231
Entity Detected:	Supt7l, suppressor of Ty 7 (S. cerevisiae)-like ( MGI:1919445)
Sequence:	sense strand is shown >T4231 GGCTTGACCTAGTCCTCGACTCCGACCTGTCAGAGTCAGGCAGCCGCGTATACCACTCCTGGGGCAGGGC GGGAGAGTATTGTCTGTCCTAGAGTTTTGCTAGCACCTTTTTAAAGTGATCTTAGCTTGCTTCTTATGTG GGAACTGCCTAGACTTCCTACTTTTCACTGCAGAAGCTTCTGATCTACAGTTGCCAGAGACGGAAGCACA CACCTTTAGGAGATCGTTTACATTAAGCTGGAGAGCCCTTCTCTGCTTGCTCTAAAATGCTGAGGTACTG GGGAGAGATACCAATACCGTCAGGACAGACCAACAGAAGTTCCTTTGATCTGCTCCCACGGGAGTTCCGT CTGGTGGAAGTCCATGACCCGCCCCTGCACCAGCCCTCAGCCAACAAGCCGAAGCCCCCCACGATGCTGG ACATTCCCTCAGAACCGTGTAGCCTCACCATCCATACCATTCAGTTGATCCAACACAACCGACGCCTGCG CAGCCTTATTGCCACAGCTCAGACCCAGAGTCAGCAGCAGACAGAGGGTGTGAAGGCTGAAGAGAGTGAA CCTCTTCCCTCCTGCCCTGGCTCACCTCCTCTCCCTGATGACCTCCAGCCTTTAGACTGTAAAAATCCCA ATGCTCCATTCCAGATCCGGCACAGTGACCCAGAGAGTGACTTTTACCGCGGGAAAGGAGAGCCTGTGAC AGAGCTGAGCTGGCACTCCTGCCGGCAGCTCCTCTATCAAGCAGTCGCCACAATCCTGGCCCACACAGGC TTTGAGTGTGCTAATGAAAGTGTCCTGGAGACCCTAACTGATGTGGCACATGAATACTGCCTCAAGTTCA CCAAGCTGCTGCGCTTTGCTGTCGACCGAGAGGCCCTGCTGGGACAGACTCCTTTCCCTGATGTAATGGA ACAGGTGTTCCATGAAGTGGGCATTGGCAGTGTGCTCTCCCTGCAGAAGTTCTGGCAGCACCGCATCAAG GACTATCACACTTACATGCTACAGATTAGTAAGCAGCTCTCTGAAGAATATGAAAGGATTGTGAATCCCG AGAAGGCCACAGAAGACACTAAACCTGTGAAGATCAAGGAAGAGCCTGTGAGCGACATCACATTTCCTGT CAGTGAGGAGCTAGAAGCGGACCTTGCTTCTGGAGATCAGTCCTTACCCATTGGGGTCCTCGGGGCTCAG AGTGAGCGCTTCCCATCCAACCTGGAGGTGGAGGCTTCGCCACAGGCTCCAAGTGCAGAGGTCAATGCTT CTCCTCTCTGGAACCTGGCTCACGTGAAAATGGAACCTCAAGAAAGTGAAGAAGGCAATGTGTCTGCGCA TGGGGTGCTGGGCAGTGATGTCTTTGAGGAGCCAATGTCAGGCATGAGTGAGGCTGGACTCCCTCAGAGC CCTGACGACTCAGACAGCAGCTATGGTTCCCACTCCACTGACAGCCTCATGGGGTCCTCCCCTGTGTTCA ATCAGCGCTGCAGGAAGAGGATGAGGAAGATTTAACTGCAGAGCGCCAGCAGGAAGCCCATGAGTAGTAA TTAGACTGTATTCTTAAGTGTAGACCTTTGCTAATGTCAGAGACAGTTCCTAACCTAGTAACAGTTCACT TTGCGTCGTTTCTGTACTTCTTTAAAACAACTAAGCCACCGTTCGCAGCTGAGTTGTGATACTGACCTGG TAATCAGACCAAAGTACTGTCCCGGCTGGTTTTATCTCAGCTCCTCTCAGCCGTGTCTCTGGCACGCTTG AGAGGAGTCATGCCACTTTGATGCTCCTTGCAGAGGTGGGACACTGAGGTGCTTTGCTTAAATTTCAAAG CTTGCATATATTCATGTAAATATATGCAATATATGACGTCTATAATAGACGTCAGCAATTCATTCGTCTA TTTTGTGTCGTCATTGGGAACCCAGTAGCAAGACAGACAGTAACAGCTAAACTTAAGTCCATCAGTCAGA GTTCAAACTATTCATGCTACTCAGGCCTCTTACAAAGTGAGAGCCGTGGGGACAGCTGTTACCCTTATGC AGCTGTCCCCAGAGTTCTTTCTTTTCTAGATTATTCCAGCCAGCCTCGTCATTTTTACTTTCCATATGCT CTTGTGCTGACCCAGACTTTTTTTTTTTTTCGAGGCAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAA CTCACTTTGTAGACCAGGCTGGCCTTGAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGGGATT AAAGGCATGTGCCACCCACGCCTGACTTTTGACCCAGACTTTTAAGTATCTTTCACCATGCACACGCTTT CACCTTTTCTCCTTGTGAAGGTGGCAAACAGGGCCCACCACAGCTGCCGGGTACGCAGCATCTGGCTTTG CTTCAGAACAGCTGTTGGTTTGTTTCAGGTGTTTGTTTTGGTTGTTTTGTTTAAAAAGACATTTGAGGAT CCAGAGAGATGGGTCAGTGGGTAAAGTCACTTGCAATTTAAGTTTGATACCGGGGACACACATGGTGTAA GGAGAGAACCAACTCCTCCATCATGACACACACACACACACACACACACACATAAAAATAAATTATTTTT GGAAAAAGTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502314 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502314 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11009 same embryo
	EMAGE:11008 same embryo
	EMAGE:11012 same embryo
	EMAGE:11011 same embryo
	EMAGE:11007 same embryo
	EurExpress:euxassay_003003 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source