EMAGE mouse gene expression spatial database

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EMAGE:11055

Os9 amplified in osteosarcoma ( MGI:1924301)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001228. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001228_01	euxassay_001228_02	euxassay_001228_03	euxassay_001228_04

euxassay_001228_05	euxassay_001228_06	euxassay_001228_07	euxassay_001228_08	euxassay_001228_09

euxassay_001228_10	euxassay_001228_11	euxassay_001228_12	euxassay_001228_13	euxassay_001228_14

euxassay_001228_15	euxassay_001228_16	euxassay_001228_17	euxassay_001228_18	euxassay_001228_19

euxassay_001228_20	euxassay_001228_21	euxassay_001228_22	euxassay_001228_23	euxassay_001228_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11055_wholemount_strong.wlz

11055_wholemount_moderate.wlz

11055_wholemount_weak.wlz

11055_wholemount_possible.wlz

11055_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11055_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 19 20 21 weak expression: see section 06

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 09 10 18 19 20 21 22 weak expression: see section 06 07 08

vagus x ganglion

moderate

regional

moderate expression: see section 09 17 weak expression: see section 08

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 17 18 19 weak expression: see section 06 07 08

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 16 17 18 19

meckel's cartilage

weak

regional

weak expression: see section 03 04 05 06 07 08 10 11 16 18 19 20 21 22

lower jaw molar

weak

regional

weak expression: see section 07 18 19 20

upper jaw molar

weak

regional

weak expression: see section 07 18 19 20 21

chondrocranium

weak

regional

weak expression: see section 02 03 04 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4187
Entity Detected:	Os9, amplified in osteosarcoma ( MGI:1924301)
Sequence:	sense strand is shown >T4187 GCGGAAGATGGCGGCGGAGGTGCTGCTGTCCAGTCTGTTGGGATTGCTATTTCTAGGGCTCCTGTTACCT GCGCGTCTGACGGGCGGTGTCGGGAGCCTGAATTTGGAGGAGCTGAGTGAGATGCGTTATGGCATCCAGA TCTTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAAGCTTCAGACGTGGTGGTTGTGTCTTCAAAGTACAA ACAGCGCTATGAGTGCCGCCTACCAGCTGGAGCTATTCACTTCCAACGTGAAAGAGAGGAGGAGACGCCT GCTTACCAGGGGCCCGGGATCCCTGAATTGTTGAGCCCAATGAGAGATGCCCCTTGTTTGCTGAAGACCA AGGACTGGTGGACATATGAATTCTGTTATGGACGTCATATCCAGCAGTACCATATGGAAGACTCGGAGAT CAAAGGTGACGTCCTCTATCTTGGCCACTACCAGTCCTCCTTCAACTGGGACGACGAAACAGCCAAGGCT TCCAAGCAGCATCGGCTGAAACGCTACCACAGCCAGACCTACGGCAACGGGTCCAAATGTGATCTCAACG GGAAGCCCCGAGAAGCTGAAGTTCGGTTCCTGTGTGACGAGGGTGCGGGCATATCTGGGGACTACATTGA CCGAGTAGATGAACCCGTCTCCTGCTCCTACGTACTGACCATTCGCACGTCAAGGCTCTGCCCGCATCCT CTCCTCCGGCCACCAGCCAGCGCTGCCCCACAGGCCATTCTTTGTCACCCAGCCCTGCAGCCTGATGAGT ACATGGCCTACCTCCAGAGGCAAGCTGAGTCAAAGCAGCATGAAGAGAAAACCACAGAGGAAGTCCAGGA CACAGACCGCCAAGTGTGGAGTGGGAGCAAGGCTGCCGGAGCACCCCCAAAGAAAGAAGATGTCAGCCCA GCCAAGGAAGAGAAGGAATCAGAGCTCTGGAAGCTTCAGGGGCCAGAGGAGCAGGCAGCAGCAAGAGAGG AGGCGCAGGCAGGGGAACAGGACCTGAACCACGAGGCCGCAGCAGATCCAGCTCCAAGCCCTCCCAATGA TTTTCAGAATAACGTGCAGGTGAAACTCATCCGGAGTCCCGCAGACTTGATTCGACTGATTGAGGAGCTG AAAGCTGCAGAAAAGGGGAAGCCAAGCGTAAGGCGGGAGCAGCCTGGAGACGATACCACGGAGGCCCCCC AGAGGGAAGCAGAGGGAACGAAGGCGAAGGGAAAGGGTGGTGAGCCCCCGGGTCTTATGGAAGAGGAGGA TGGTGACGACGAAGAGGAGGAGGAGGAGGAGGAAGAGGACGAGGAGGAGCAGCAGCTCCTGGGAGAGTTC GAGAAGGAGCTGGAGGGGATGTTGCTGCCCTCAGACCGCGAGCGCCTCCGCTCTGAGGTTAAGGCTGGCA TGGAGCGGGAGCTGGAGAACATCATCCAGGAGACAGAGAAGGAACTGGACCCAGAAGGGCTGAGGAAGGA GTCCGAGCGGGAGCAGGCAATATTGGCTCTAACATCCACTCTGGACAAACTCATCAAGAGGCTGCAGGAG AACCAGAGTCCAGAGCTTGTGCAAAAATACAAAAAAAGGAGAGTTGTCCCCCAAAAGCCTCCCCCATCAC CACACCCTACAGGCAAGATTGAGATCAAGATTGTGCGACCCGGGGCTGAAGGTAAGGAAGAGGACACACG CTGGCTGACTGATGAGGATACAAGAAACCTTAAGGAGATTTTCTTCAATATCTTGGTACAGGGAGCCGAA GAGGCCAATAAAGAACGCCAGCGACAGAGTGAGCTGGAGAGCAACTACCGCCGGGTGTGGGGCTCTCCCG GTGGTGAAGACACGGGGGACCTGGATGAATTTGACTTCTGAGACCACCCTGCCAGCCCTCTAAGGATTCC TGCATCTTCCTGACCGGCCGGCTCTGCCTCCTTACACCCCATTCTGGGGCCTTGGAGGCAAAAGGGCAGA TTGGAGCCCAGCACTGGACTTGAGGGGCCCAGCCCCAAGAGTGTATGAGGGTGTGTGGGGACCCATGAGG GTGAGGAGCTGTTGCAGCCAGGCCTATGGGGTCCACTCCCCAAACACACCCTCCTTTGCCCCTTTAGCTT TTTCCTGTCCCCCAACTTCTTAGCACTTAACCCCACTCTACCCAGAGACTTGATGAGACCTTGTGAGTGG TGGTGGCAGGAACAGCAGGGCTGTGCCGGTCCCCGACCCCTCCTCTCCCTATCCTACCTCCATTCCCTGT TGCTGTTTGGGAGCTGTTGCCCCTCCCTTGGAACGCCTACTTCCTGTCTCTGCTGTCTCCCGGTACCTGT GCTTCTGAGCCCAGACTATCTCTTGTCACTGAGGGGATGGGAGACCTTTCATATCACCTCACAAACAATC CCAGCAAAAAGGGTTGGAGATGGAGCACCCCCTTGTTCTTCAGCAATCAGGTTTCTAAATAAAAAACTGG ACCGTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4487829 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4487829 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11058 same embryo
	EMAGE:11057 same embryo
	EMAGE:11059 same embryo
	EMAGE:11054 same embryo
	EMAGE:11056 same embryo
	EurExpress:euxassay_001228 same experiment
	MGI:4826986 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source