EMAGE mouse gene expression spatial database

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EMAGE:11056

Lilrb3 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 ( MGI:894311)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003226. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003226_01	euxassay_003226_02	euxassay_003226_03	euxassay_003226_04

euxassay_003226_05	euxassay_003226_06	euxassay_003226_07	euxassay_003226_08	euxassay_003226_09

euxassay_003226_10	euxassay_003226_11	euxassay_003226_12	euxassay_003226_13	euxassay_003226_14

euxassay_003226_15	euxassay_003226_16	euxassay_003226_17	euxassay_003226_18	euxassay_003226_19

euxassay_003226_20	euxassay_003226_21	euxassay_003226_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11056_wholemount_strong.wlz

11056_wholemount_moderate.wlz

11056_wholemount_weak.wlz

11056_wholemount_possible.wlz

11056_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11056_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4192
Entity Detected:	Lilrb3, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 ( MGI:894311)
Sequence:	sense strand is shown >T4192 GGGAGATGCCATGTCCTGCACCTTCACAGCCCTGCTCTGTCTTGGACTGACTCTGAGCCTCTGGATCCCA GTGCTGACAGGGTCCCTCCCTAAGCCTATCCTCAGAGTACAGCCAGACTCTGTGGTCTCCAGGTGGACTA AGGTGACTTTCTTTTGTGAGGAGACAATTGGAGCCAATGAGTACCGCCTCTATAAAGATGGAAAGCTATA TAAAACTGTAACAAAGAACAAACAGAAGCCAGCAAACAAGGCTGAATTCTCACTCTCAAATGTAGACCTG AGTAATGCAGGTCAATATGAATGTTCCTACAGCACCCAGTATAAATCATCAGGCTACAGTGACCCCCTGA AGCTGGTGGTGACAGGACACTACTGGACACCCAGCCTTTTAGCCCAAGCCAGCCCTGTGGTAACTTCAGG AGGGTATGTCACCCTCCAGTGTGAGTCCTGGCACAACGATCACAAGTTCATTCTGACTGTAGAAGGACCA CAGAAGCTCTCGTGGACACAAGACTCACAGTATAATTACTCTACAAGGAAGTACCACGCCCTGTTCTCTG TGGGCCCTGTGACCCCCAACCAGAGATGGATATGCAGATGTTACAGTTATGACAGGAACAGACCATATGT GTGGTCACCTCCAAGTGAATCCGTGGAGCTCCTGGTCTCAGGTAATCTCCAAAAACCAACCATCAAGGCT GAACCAGGATCTGTGATCACCTCCAAAAGAGCAATGACCATCTGGTGTCAGGGGAACCTGGATGCAGAAG TATATTTTCTGCATAATGAGGGAAGCCAAAAAACACAGAGCACACAGACCCTACAGCAGCCTGGGAACAA GGGCAAGTTCTTCATCCCTTCTATGACAAGACAACATGCAGGGCAATATCGCTGTTATTGTTACGGCTCA GCTGGTTGGTCACAGCCCAGTGACACCCTGGAGCTGGTGGTGACAGGAATCTATGAACACTATAAACCCA GGCTGTCAGTACTGCCCAGCCCTGTGGTGACAGCAGGAGGAAACATGACACTCCACTGTGCCTCAGACTT TCACTACGATAAATTCATTCTCACCAAGGAAGATAAGAAATTCGGCAACTCACTGGACACAGAGCATATA TCTTCTAGTAGACAGTACCGAGCCCTGTTTATTATAGGACCCACAACCCCAACCCATACAGGGACATTCA GATGTTATGGTTACTTCAAGAATGCCCCACAGCTGTGGTCAGTACCTAGTGATCTCCAACAAATACTCAT CTCAGGGCTGTCCAAGAAGCCCTCTCTGCTGACTCACCAAGGCCATATCCTGGACCCTGGAATGACCCTC ACCCTGCAGTGTTACTCTGACATCAACTATGACAGATTTGCTCTGCACAAGGTGGGGGGAGCTGACATCA TGCAGCACTCTAGCCAGCAGACTGACACTGGCTTCTCTGTGGCCAACTTCACACTGGGCTATGTGAGTAG CTCCACTGGAGGCCAATACAGATGCTATGGTGCACACAACCTTTCCTCTGAGTGGTCAGCCTCCAGTGAG CCCCTGGACATCCTGATCACAGGACAGCTCCCTCTCACTCCTTCCCTCTCAGTGAAGCCTAACCACACAG TGCACTCAGGAGAGACCGTGAGCCTGCTGTGTTGGTCAATGGACTCTGTGGATACTTTCATTCTGTCCAA GGAGGGATCAGCCCAGCAACCCCTACGACTAAAATCAAAGTCCCATGATCAGCAGTCCCAGGCAGAATTC TCCATGAGTGCTGTGACCTCCCATCTCTCAGGCACCTACAGGTGCTATGGTGCTCAAAACTCATCTTTCT ACCTCTTGTCATCTGCCAGTGCCCCTGTGGAGCTCACAGTCTCAGGACCCATCGAAACCTCTACCCCGCC ACCCACAATGTCCATGCCACTAGGTGGACTGCATATGTACCTGAAGGCTCTCATTGGAGTGTCTGTGGCC TTCATCCTGTTCCTCTTCATCCTCATCTTCATTCTTCTCCGACGAAGACATCGGGGAAAATTCAGGAAAG ATGTCCAGAAAGAGAAAGACTTGCAACTTTCTTCAGGAGCTGAAGAGCCCATAACCAGGAAAGGAGAACT CCAGAAGAGGCCCAACCCAGCTGCTGCCACCCAGGAAGAAAGCCTATATGCTTCAGTGGAGGACATGCAA ACTGAGGATGGAGTGGAGCTGAACAGCTGGACACCACCTGAGGAAGATCCCCAGGGAGAGACTTATGCCC AGGTGAAACCCTCCAGGCTCAGGAAGGCAGGACATGTCTCACCTTCTGTCATGTCAAGGGAACAACTGAA CACAGAATATGAACAAGCAGAAGAGGGCCAAGGAGCAAACAATCAGGCTGCCGAATCTGGGGAGTCCCAG GATGTGACCTATGCCCAGCTGTGCAGCAGGACACTCAGACAGGGGGCAGCTGCATCTCCTCTCTCCCAGG CAGGGGAAGCCCCAGAGGAGCCCAGTGTATATGCTACCCTGGCGGCTGCTCGTCCAGAGGCTGTTCCCAA GGATGTGGAGCAATGACCCCCTGCCTGCCAGGATGCCTAGCAGAGACCTCCAAGGGACTCTGGGAACTTT TGGAAACGTGACTGCACTTTAAGTAACATCAGAGTTTGTAAATAAAGCTAGAGATTTCTCAATAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4488338 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4488338 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11055 same embryo
	EMAGE:11058 same embryo
	EMAGE:11057 same embryo
	EMAGE:11059 same embryo
	EMAGE:11054 same embryo
	EurExpress:euxassay_003226 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source