EMAGE mouse gene expression spatial database

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EMAGE:11059

Fam83a family with sequence similarity 83, member A ( MGI:2447773)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003187. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003187_01	euxassay_003187_02	euxassay_003187_03	euxassay_003187_04

euxassay_003187_05	euxassay_003187_06	euxassay_003187_07	euxassay_003187_08	euxassay_003187_09

euxassay_003187_10	euxassay_003187_11	euxassay_003187_12	euxassay_003187_13	euxassay_003187_14

euxassay_003187_15	euxassay_003187_16	euxassay_003187_17	euxassay_003187_18	euxassay_003187_19

euxassay_003187_20	euxassay_003187_21	euxassay_003187_22	euxassay_003187_23	euxassay_003187_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11059_wholemount_strong.wlz

11059_wholemount_moderate.wlz

11059_wholemount_weak.wlz

11059_wholemount_possible.wlz

11059_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11059_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

strong

homogeneous

strong expression: see section 12 15 16

anterior naris

strong

homogeneous

strong expression: see section 12 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4186
Entity Detected:	Fam83a, family with sequence similarity 83, member A ( MGI:2447773)
Sequence:	sense strand is shown >T4186 CCACGCGTCCGGGAGGAAGGAACTCGGCAAGCCAGTATGGGAAGCCCATAGCTTCCTAGGAATACTGGGA GCCCTATTCCTCCCTGGTGTCGTCCGCATTCTTCCTACGTCTGGAAGAGCTCCGCGGTTGGCCACCCCTC CACGACTGAGCAACACAGCAGCATGAGTCGGTCAAGGCATGTGGGCAAGATCCGGAAACGTCTGGAAGAT GTCAAGAACCAGTGGACCCGGCCAGCCAGAGCTGACTTCAGTGATAATGAGAGCGCCCGGCTGGCCACAG ATGCCCTCTTGGATGGCGGTCCAGAGGCTTACTGGCGCGCCCTCAGTCAGGAAGGAGAGGTAGACTTCCT GTCCTCAGCGGAGGCCCAGTACATCCAGGCCCAGGCCAAGGAGCCCCCTGATGCCCCCGATTCTGCCGGA GGGGCTGAGTCAGGACCCAGAGGACTGGACTCCTGCTCTTTACAATCAGGTACCTACTTCCCTGTGGCTT CGGAAGGCAGTGAGCCAGCTCTGCTGCACACCTGGACTTTGGCTGAGAAGCCCTACCTGAAGGAAAAGTC CAGTGCCACCATCTACTTCCAAATGGACAAACACAACAACATCAGAGATTTGGTCCGCCGCTGCATCTCC CGGGCCAGCCAGGTCCTGGCCATTCTGATGGATGTGTTCACGGATGTGGAGATCCTTTGTGACATTCTAG AGGCAGCCAACAAGCGCGGGGTATTCGTCTGTGTGCTTCTGGACCAGGCCGGGGTGAAGCTCTTCCAGGA GATGTGTGACAAAGTCCAGATCTCTGATATTCATCTCAAGAACATCTCCATCAGGAGTGTGGAAGGGGAG GTGTATTGTGCCAAGTCAGGCAGGAAATTTGCCGGCCAAATCCAAGAGAAGTTCATCATCTCAGACTGGA GGTTTGTCTTATCCGGATCATACAGGTGAGTACTGGTTTCATCTGCTGTCCAGGCTTCCGGGCAGTGATG GGCTAAGTGGGGTACCAGCTGGATGATGGCTGCTCCCACCCCACCCCCCCCCAGAGCTCCCAGCTCTTCT TTCTGGCCACAGCTAGAGCTCACCAAATAAACGAAGCCAGAAGTGAGAAATGTGGCCCCTTCCTGACACT GAAATTGGACAGAGACTTCTTTCTACTCTCTCCGGCCTCTTAGGAATTGTGATAAAACAATAGATTTGCT TTTAACTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4487327 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4487327 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11055 same embryo
	EMAGE:11058 same embryo
	EMAGE:11057 same embryo
	EMAGE:11054 same embryo
	EMAGE:11056 same embryo
	EurExpress:euxassay_003187 same experiment
	MGI:4824764 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source