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EMAGE:11064

Gm2382 predicted gene 2382 ( MGI:3780550)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11064 EMAGE:11064 EMAGE:11064 EMAGE:11064 EMAGE:11064
"Pseudo-wholemount" of euxassay_003085. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003085_01 euxassay_003085_02 euxassay_003085_03 euxassay_003085_04
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View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11064Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11064_wholemount_strong.wlz
11064_wholemount_moderate.wlz
11064_wholemount_weak.wlz
11064_wholemount_possible.wlz
11064_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11064_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4679
Entity Detected:Gm2382, predicted gene 2382 ( MGI:3780550)
Sequence:sense strand is shown

>T4679
CTTGTGAGGGAGCGAGCTGAGCTTCCGGGCAGGTGATTGCCCACAATCAGTATCAGAATTCCAAAAGAAT
TTCCATCTTTCTGAGCATGCAAGATGAAGTTGAGACGGAAGTAATCATCAAGGACATTTTCAAACAAGGC
AAAATCTGCTTCATCCCTCAGTACCAATTCCAGAGCAATCACATGGACATGGTGAGGTTAACGTCACCTG
AAGAAATTGCTTTACTTCCCAAGACATCTTGGAATATTCATCAGCCTGGTGAGGGAGATGTTCGGGAGGA
GGCCTTATCCACTGGTGGACTTGACCTCATCTTCCTTCCTGGCCTCGGGTTTGACAAAGATGGCAACCGG
CTGGGGCGGGGTAAGGGGTACTATGACACCTACCTGAAGCGCTGTGTACAGCATCAGGAAGTGAAGCCCT
ACACCATGGCTTTGGCTTTTAAAGAGCAGATCTGTCCCCAGATCCCAGTGGATGAGCATGACATGAAGGT
AGATGAAGTCCTTTATGAAGATTCCCCAGCATCCTAAGTTACACAACGACGGCTACACTTACTGCTCTGT
CCCGGTGTAAAGAACATATGTATATTTTTCTCTTGTCAAAATGAATCAAAATGGCACATTAAAGTGAATA
TGGGTTGTGATTTTAAGTGTAAAAATAAAATAATATAAAATCCTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5026828 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5026828 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11065 same assay
 EMAGE:11063 same embryo
 EMAGE:11067 same embryo
 EMAGE:11062 same embryo
 EMAGE:11066 same embryo
 EurExpress:euxassay_003085 same experiment
 MGI:4826493 same experiment
 MGI:4826492 same experiment
 EMAGE:11061 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS