EMAGE mouse gene expression spatial database

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EMAGE:11094

Tmbim6 transmembrane BAX inhibitor motif containing 6 ( MGI:99682)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003552. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003552_01	euxassay_003552_02	euxassay_003552_03	euxassay_003552_04

euxassay_003552_05	euxassay_003552_06	euxassay_003552_07	euxassay_003552_08	euxassay_003552_09

euxassay_003552_10	euxassay_003552_11	euxassay_003552_12	euxassay_003552_13	euxassay_003552_14

euxassay_003552_15	euxassay_003552_16	euxassay_003552_17	euxassay_003552_18	euxassay_003552_19

euxassay_003552_20	euxassay_003552_21	euxassay_003552_22	euxassay_003552_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11094_wholemount_strong.wlz

11094_wholemount_moderate.wlz

11094_wholemount_weak.wlz

11094_wholemount_possible.wlz

11094_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11094_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6786
Entity Detected:	Tmbim6, transmembrane BAX inhibitor motif containing 6 ( MGI:99682)
Sequence:	sense strand is shown >T6786 TAGCAGAGCAGGGAAGCTCCGGAGAGGTGGGCTGAGAAGCGGAGGCTGCTGAACAGACTTTGGAGCCATG AATATATTTGATCGGAAGATCAACTTTGATGCCCTCTTAAAATTTTCCCACATAACTCCCTCGACACAGC AGCACCTAAAGAAGGTCTATGCCAGTTTTGCACTATGTATGTTTGTGGCGGCAGCAGGGGCCTATGTCCA TGTGGTCACACACTTCATCCAGGCTGGCCTACTCTCCGCCCTGGGCTCCTTGGCCTTGATGATTTGGCTG ATGGCAACACCTCACAGCCATGAGACCGAGCAAAAGAGACTGGGACTTCTCGCTGGCTTCGCCTTCCTCA CAGGAGTCGGCCTGGGACCTGCCCTGGAGCTGTGCATCGCTGTCAACCCCAGCATCCTCCCCACGGCCTT CATGGGCACGGCCATGATCTTCACCTGCTTCAGCCTGAGCGCCCTGTATGCCCGGCGCCGGAGCTACCTG TTTTTAGGAGGTATCTTGATGTCAGCCATGAGCCTCATGCTCCTGTCCTCTCTGGGGAACCTTTTCTTTG GCTCCATTTGGCTGTTCCAGGCAAACCTGTACCTGGGACTGCTGGTCATGTGTGGCTTTGTCCTCTTTGA TACTCAGCTCATTATTGAGAAGGCTGAACACGGAGATAAGGATTACATCTGGCACTGCGTTGACCTCTTC CTAGATTTCGTTACCCTCTTCAGGAAGCTCATGTTGATCCTGGCCTTCAATGAGAAGGACAAGAAGAAAG AAAAGAAGTGACCAACTGGCCGTCAGCCTTTCCCAGCTCACCTTCTCCCCCCCACCCCCCCACCCCCGTT TCTTTGCACACATCACAGGTGTCGTGTTCTATGATAATGAAAGCATCAGGAAAGCTTTTGTACTTAGTGG TTTTCTCTATTTTGAAATTTTTTTGATCAAAAACTGATTAGTGAATATAGTTTGGAGTTTGGCTTCGTGT CCTGGGTTCTTCCCCGTCCCTTTTCTGTCAGCCCATCACAGCCACCTGCTCTGCTCAGACAGCCAGGCAC CAGAAGTGAGAGCAGAAGTCTGCATCCTGCCGAGCTGCCGCAGACGGAGCCCCGCTGCTGACTGGACCCT CTCTGGCCCCGTGGTTCGGTTCTCACAGTTCCAGTGAGACTGGGCAGCGTGTTTGCCTGTCCTGAGGTGC CAGGCCTGCAGAGAGTGGACTCTGGTGGGTGCTTCCTTCCTCTGCTGCCTAACTGAGTCTGGCACAGGCT TTCTGCCTTGCCATGAGGAGGAGGTGTAGACTTGTGAGTGCTCTCTCCGCTCTGAGTGGCCCTCGGGGAA CCACCTGCTTTCCATCTGTGGGGTGGGGCGCTCGCTCCGAGTATGTGTGGCAAGGTGGAGGCCACAATGC CAAGGTTCCCTAGTGTGTTTTTCTTTGTTGGTTCTTAAAATTATCAACCTTTGTTACGGATGGGCCTCCA CTAGGTGGCTGTGGGTAAAGTCTTGGGGGGAGGATAGGAAAGGAGACCGGCTGAGCCTCAGGGCTAATTG TACTGAGTAAGGACTGCAGCTCCCGGTGACCGCGAGGAGTCGGTGAGGTGGGACTGGTGACTCTCAGAAG CTCCTCGGTGCACTTTTGTCTCGGCAGAGCTGGGAGGGAGTAGGCGCTACGTCACTTACTGGGTTTGTTC ACCTGTTTCCAGCAAGTTTTGGTCTTTTGGGCAGAAGCCTGTTGACCAACTGTGGGCCACCACAGTCTGC ACAGAAAGGTGGCACCCGGAGTGGTTGTGGCCCTCACTACCAAGCCACGGGCAGCCCATTTCCAGTAGGA TGGCGGTTTGGATTTCTTTGCTCAGAGCCAATGCAGTTGACACAGTGGCTGCTGGGTTACTGTCCCATGA GACTGCATTGGACGGGTGCAGCCAGAGTGGCGCAGTGCACTAAGGTGCCGGCCGCCCTGCCTGCCCGGTG CCTCCCACTGACCTGAGCTTACTAAGGGATGGCAGCAGCTCCCCAGAAAAAAACGACACTGCGAGCCCTC GGGACATGACTGAGTTCCACCTCCTGAAGCCAGACTCCACCTCTTGTGCTTTTTTGCTTGGGAAAAAGGA GTTTTTCTTTAGAAACAGTGCCAAGAATGATATAAACTACTTTTAACTAAGATGCCTAACAGGCTTTTAA CACAGGTGTCCATCTCCACTCTGTCCCCGGTCTCTGTCCCAGCTCAGGCTGCGTTCTCTAACTCATACTG TGAAGACAACCGTGTTTTTGATTAAAAAAAATATATCTACATAGTCTTAATTTGTAAAAAATAAAGAAAA TTCCTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3708463 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3708463 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11097 same embryo
	EMAGE:11095 same embryo
	EMAGE:11098 same embryo
	EMAGE:11096 same embryo
	EurExpress:euxassay_003552 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source