EMAGE mouse gene expression spatial database

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EMAGE:11097

Sepsecs Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase ( MGI:1098791)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003506. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003506_01	euxassay_003506_02	euxassay_003506_03	euxassay_003506_04

euxassay_003506_05	euxassay_003506_06	euxassay_003506_07	euxassay_003506_08	euxassay_003506_09

euxassay_003506_10	euxassay_003506_11	euxassay_003506_12	euxassay_003506_13	euxassay_003506_14

euxassay_003506_15	euxassay_003506_16	euxassay_003506_17	euxassay_003506_18	euxassay_003506_19

euxassay_003506_20	euxassay_003506_21	euxassay_003506_22	euxassay_003506_23	euxassay_003506_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11097_wholemount_strong.wlz

11097_wholemount_moderate.wlz

11097_wholemount_weak.wlz

11097_wholemount_possible.wlz

11097_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11097_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 05 24

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pectoral girdle and thoracic body wall musculature

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 19 20 21 22 23 24 moderate expression: see section 17 18

kidney calyx

strong

regional

strong expression: see section 08 09 10 11 18 19 20 21 22 moderate expression: see section 07

male reproductive system

strong

regional

strong expression: see section 10 11 12 18 19

genital tubercle of male

moderate

regional

moderate expression: see section 13 14 15 16 17

thyroid cartilage

strong

regional

strong expression: see section 13 14 15 16 moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7935
Entity Detected:	Sepsecs, Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase ( MGI:1098791)
Sequence:	sense strand is shown >T7935 GTCATGAACCCGGAGAGCTTCGCGGCGGGCGAGCGGCGGGTGTCTCCGGCTTACGTGAGGCAGGGCTGCG AGGCTCGGCGCGCCCACGAGCACCTCATCCGGCTGCTTCTAGAGCAGGGCAAGTGTCCAGAGGATGGCTG GGATGAAAGTACCCTCGAACTCTTTCTGCATGAGCTTGCAGTCATGGATAGCAACAATTTCTTGGGTAAT TGTGGTGTGGGAGAAAGGGAAGGGAGAGTGGCCTCTGCTTTGGTTGCTCGACGACATTACAGGTTCATCC ACGGAATTGGACGATCCGGTGATATTTCTGCTGTGCAACCAAAAGCTGCAGGCTCTAGCCTCTTGAACAA AATTACTAATTCTTTGGTGCTTAATGTCATAAAGCTTGCTGGTGTTCATTCAGTGGCCAGTTGCTTTGTA GTTCCTATGGCAACTGGGATGAGTCTAACCTTGTGTTTCCTGACACTACGACACAAAAGACCAAAGGCCA AGTATATCATATGGCCACGGATAGACCAGAAGTCCTGCTTTAAGTCCATGGTCACTGCAGGTTTTGAACC CGTGGTAATAGAAAACGTATTGGAAGGTGATGAGCTGCGCACAGACCTGAAAGCCGTGGAGGCTAAAATC CAGGAGCTCGGGCCGGAGCACATCCTGTGCCTTCATTCTACCACAGCCTGCTTTGCTCCAAGGGTGCCCG ATAGATTAGAAGAACTGGCTGTGATTTGTGCTAATTATGACATTCCACATGTAGTCAACAACGCTTATGG ATTACAGTCTTCAAAGTGCATGCATCTCATTCAGCAGGGGGCTCGGGTTGGTAGAATTGATGCTTTCGTT CAGAGCTTGGACAAAAATTTTATGGTTCCAGTAGGTGGTGCTATAATTGCTGGCTTTAATGATCCCTTCA TTCAGGACATCAGCAAGATGTATCCAGGAAGAGCCTCAGCCTCGCCGTCCTTAGACGTCCTCATCACCTT GCTGTCACTGGGCTGCAGTGGCTACAGGAAGCTGTTGAAGGAGAGAAAGGAAATGTTTGTCTATTTATCC ACCCAACTAAAGAAGTTGGCAGAGGCCCACAATGAAAGACTACTACAGACACCTCACAACCCTATATCCT TAGCTATGACACTTAAGACGATAGATGGACACCATGACAAAGCTGTCACTCAGCTTGGCTCAATGCTTTT TACCAGACAAGTGTCTGGAGCCAGGGCTGTGCCTCTTGGGAACGTGCAAACTGTGAGTGGCCATACTTTT CGAGGCTTCATGTCCCATGCAGATAATTACCCCTGTGCTTACCTCAATGCGGCAGCAGCCATCGGGATGA AGATGCAGGATGTGGACCTGTTCATAAAGAGACTTGACAAGTGCTTAAACATAGTAAGGAAAGAACAGAC TAGAGCAAGTGTTGTGTCTGGAGCTGACCGCAACAAAGCTGAAGATGCGGACATTGAGGAAATGGCTTTG AAACTGGATGACGTGCTTGGGGATGTGGGCCAGGGCCCTGCTCTATGACACAGGGCTCTTTTTGACAGTT CATACAATGTATGTAAAGTTTTTGAATCAAGAATTTTATAGAGAGTATTCTGCCAAGGAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6516578 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6516578 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11095 same embryo
	EMAGE:11098 same embryo
	EMAGE:11096 same embryo
	EMAGE:11094 same embryo
	EurExpress:euxassay_003506 same experiment
	MGI:4827957 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source