EMAGE mouse gene expression spatial database

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EMAGE:11140

Rogdi rogdi homolog (Drosophila) ( MGI:1913299)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003486. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003486_01	euxassay_003486_02	euxassay_003486_03	euxassay_003486_04

euxassay_003486_05	euxassay_003486_06	euxassay_003486_07	euxassay_003486_08	euxassay_003486_09

euxassay_003486_10	euxassay_003486_11	euxassay_003486_12	euxassay_003486_13	euxassay_003486_14

euxassay_003486_15	euxassay_003486_16	euxassay_003486_17	euxassay_003486_18	euxassay_003486_19

euxassay_003486_20	euxassay_003486_21	euxassay_003486_22	euxassay_003486_23	euxassay_003486_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11140_wholemount_strong.wlz

11140_wholemount_moderate.wlz

11140_wholemount_weak.wlz

11140_wholemount_possible.wlz

11140_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11140_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 16 17 18 19

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 15 16 17

kidney calyx

moderate

regional

moderate expression: see section 07 08 09 10 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6674
Entity Detected:	Rogdi, rogdi homolog (Drosophila) ( MGI:1913299)
Sequence:	sense strand is shown >T6674 CGGACGCGTGGGCGGCTAGGAGGATGGCGACCGCGATGGCTGCGAGCGCCGCGGAGCGGGCAGTACTGGA GGAGGAGTTTCGCTGGTTACTGCACGCTGAGGTGCACGCCGTGCTGAGACAGCTGCAGGACATCCTCAAG GAGGCCTCTCTCCGCTTCACTCTCCCAGGCCCCAGCACCGAGGGCCCTGCCAAGCAGGAGAACTTCATCC TGGGCAGCTGTGGCACAGACCAGGTGAAAGGGACTCTGACTCTGCAAGGGGATGCCCTCAGCCAAGCGCT CCCTCCTGGCTGGCTGAGGCGCCTAGGGCCTCAGCCATCCCCCCACTGCATTGCTGGCTCACAGGATGTG AACCTGAAGATGCCTCGGAACAACCAGTTGTTGCACTTGGCCTTCCGGGAAGACAAGCAGTGGAAGCTGC AGCAGATCCAGGATGCCAGGAACCATGTGAGCCAAGCTATTTATCTCCTAGCAAACCGGGATGAGAGCTA CCAGTTCAAGACAGGAGCGGAGGTCCTCAAGCTCATGGATGCTGTTATGCTGCAACTGACTAGAGCCAGA AGCCGGCTCACTACCCCAGCCACCCTCACCCTGCCGGAGATTGCTGCCAGCGGCCTCACGCGGATGTTTG CACCTACCCTACCCTCTGACCTGCTGGTCAATGTCTACATCAACCTCAACAAGCTGTGCCTCACTGTATA CCAGCTGCACGCCCTGCAACCCACCTCCACCAAGAACTTCCGCCCGGCCGGAGGCGCAGTGCTCCACAGC CCCGGCGCTATGTTTGAGTGGGGCTCACAGCGTCTCGAGGTGAGCCACGTGCACAAGGTAGAATGTGTGA TACCCTGGCTCAACGATGCCCTGGTATACTTCACCGTCTCCCTGCAACTCTGCCAGCAGCTCAAGGACAA GATCGCTGTGTTTTCCAGCTACTGGAGCTCCCGGCCCTTCTGAGCAAGCACCTGCCTGACTCCTCCCCCA AGATGGTAGCCCTGACTTGTAGCCCCTCCCCACAACTCGTACCCATGCAGTGCCTGTCACTGTCACTTTT CTCCCTCCTCATTCCCTGTGGTACTGATGGCAGTGAACCTGAGAGGCACCTGGAGAAGTGAGTGCAGGCT TGGTGTTTTGATTAGGGGAGGGGTCAGTCAAGAAGATGAGCTGAGGTGTGACCATGCTCCTAACCCTGCC TTCCTGGACACTCCAGGAACCTTTGGACTAAGGTGGGACCAAGCCTAGGCCTCTCCCTTCTCCTACCCTC TCAGCTCCCATTAGGGAGCCCTTGAGGGATGGGGTTAGCACTCACCTCTGAGGTGGAGGAGGGAAGAGGG ACCTGGGCTGCGGAAGAACCTCCCTGTAAATCAGGTGCAGTACTGGGACCATCTGTTCTGTGACCCCACC CACCTCTAAGAAATAAAAATCAGGTTTGACTCTGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601096 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601096 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11142 same embryo
	EMAGE:11144 same embryo
	EMAGE:11143 same embryo
	EMAGE:11145 same embryo
	EMAGE:11141 same embryo
	EurExpress:euxassay_003486 same experiment
	MGI:4827788 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source