EMAGE mouse gene expression spatial database

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EMAGE:11142

Dok4 docking protein 4 ( MGI:2148865)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003382. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003382_01	euxassay_003382_02	euxassay_003382_03	euxassay_003382_04

euxassay_003382_05	euxassay_003382_06	euxassay_003382_07	euxassay_003382_08	euxassay_003382_09

euxassay_003382_10	euxassay_003382_11	euxassay_003382_12	euxassay_003382_13	euxassay_003382_14

euxassay_003382_15	euxassay_003382_16	euxassay_003382_17	euxassay_003382_18	euxassay_003382_19

euxassay_003382_20	euxassay_003382_21	euxassay_003382_22	euxassay_003382_23	euxassay_003382_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11142_wholemount_strong.wlz

11142_wholemount_moderate.wlz

11142_wholemount_weak.wlz

11142_wholemount_possible.wlz

11142_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11142_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

spleen primordium

moderate

regional

moderate expression: see section 04 05 06 07

facial vii ganglion

strong

regional

strong expression: see section 05 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6232
Entity Detected:	Dok4, docking protein 4 ( MGI:2148865)
Sequence:	sense strand is shown >T6232 CTGGCGGCTGCGCTTCACTCTAGCTTACAACCAGAGCCGCGCGCCGCCACCCTACCTTCCCCTCCCGGCC GCGCCGAGACGCGGGCATGAGCCCGCCCCGCGTCCCGCGGAGCCAGCGCCCCGGCCCGGGCCTCGGCGCC TAGGAGCTGGGCGAAGGATCTGCTAGAACAATGCTGAGGCGGGGGAGGTGAGGAGTGTCTTAAACAGGTG ATCGGGGGACACCAAGCCACCTGGGCACCCCTCCCGGACTCAGCCCGGCCGGCACCCCAAAGACAGCGGG GCAACCATGGCGACCAATTTCAACGACATCGTCAAGCAAGGCTATGTGAAGATGAAGAGCAGGAAGCTCG GGATTTACCGGAGGTGCTGGCTGGTGTTCCGGAAATCTTCCAGCAAGGGGCCCCAGCGGCTGGAGAAGTA TCCTGATGAGAAGTCCGTGTGCCTCCGAGGCTGCCCCAAGGTGACTGAGATTAGCAACGTCAAGTGTGTC ACACGGCTCCCCAAGGAGACCAAGAGGCAGGCGGTGGCCATCATATTCACAGACGACTCCGCTCGCACCT TCACTTGTGACTCAGAGCTGGAGGCAGAAGAGTGGTACAAGACACTGTCCGTGGAATGTCTGGGATCACG GCTCAATGACATCAGCCTGGGAGAGCCTGACCTCCTGGCTCCAGGAGTACAGTGTGAGCAGACAGATCGC TTCAACGTCTTCCTGTTACCCTGCCCCAACCTGGACGTGTATGGGGAGTGCAAGCTGCAGATCACTCACG AGAACATCTACCTCTGGGACATACACAACCCCCGCGTGAAGCTCGTCTCGTGGCCCCTCTGCTCTTTGCG CCGTTATGGCCGAGATGCTACGCGCTTTACCTTTGAGGCGGGCAGGATGTGTGACGCTGGGGAAGGGCTC TATACCTTTCAGACACAGGAGGGGGAGCAGATTTACCAGCGGGTTCACAGTGCCACCCTGGCCATCGCCG AGCAGCACAAGCGGGTCCTGCTGGAGATGGAGAAGAATGTGAGGCTGCTGAACAAGGGCACCGAGCACTA CTCCTATCCCTGCACACCCACGGCCATGCTGCCCCGCAGCGCGTACTGGCACCATATCACAGGTTCTCAG AACATTGCTGAAGCCTCCAGCTATGGGGAAAGTTATGGGGCAGCCCAGGCCAGCTCGGAAACAGACCTCC TGAACAGGTTCATCTTGCTTAAGCCAAAGCCCAGTCAGGAGGATAGCAGTGAGGCCAAGACCCCTGCCCA GTGACAGCCAGACTGGAGAGAACTCATGATTTGCTGGGCCACCTGTTGAAAGGCTCACTGTCGCAGCCCA TGGACAGCCTCGAGGAGCTGGTGGCCAAGAGCCAGAGGAGAGCAAGCTATCCCTCCTTGCCCTCAAGGGT GAGGCTGGACCTCAGCACATGGCTGGCCACACCACCTGAGCATGAGGGGCTAAGAGCCACCGTGGGACTA TACACTGTTAATGGTTTTAATATATATGGCTTATAACATATACTGTATAAACGAGGCTTCCCTCTATCCC TCCCCACCAACACATACCCATTTTTAATTCCACGACCAGGCATGGTCAAGGCTGTTTTTGAGTATGAGGG TGGCAGTGCCTGTCCTCAGTGATACAACTCTCCTGCTGCCAAGATGGCCTGAGCTGCCAGGGTCAGAGGA GGGGGACAATCAGACTGGTGCTGTCTGCTGGCTCTTCCTCAGAAGCCCAAGGGCCAGGACAGCAGGTAGC TCTGTCCTCTGGGCCTACAGTGACACAGGAAGAGTCCTAAACTTCACTGTACCGTGTGGCAGCCTTGCAC TTGGGAGTGTTTAGCAGAGGCGGCATCTGGTTTTTAAATGCACTTTCTGCACTGGCCCCTTCCTGCTCAG TAGGCTGAAGATGTCTTTGGGCAGGGCTCCCGTTACCTCGGCAGCAGAGGACAGTTGGGGCCAGCCCGGA GGGAGGACTGCAGCAGGACTGCTGTGGGGAGCACCTTGCTGCTCTCCTTCACTGACCAGGGGTATAAAGC TCCAGCAGGGGGAGTCCCTTGCCCAGGCCAGCCACCCCAGGCCAGATTCCAAAACTAAGACTTTCCTATC TCCCCACACGGTGCTGAGGCTCCCTGCTGAAATGCAATTAAAGCAATTGATTTTCTAGTGCTGGTATTTA TTGACTACCGTGCAGAAGGGCTATCTTTCTATTCACATCAAACCTTTTGTTGCGTGTTTCTGTTTTTTAA TACTTTAGGGTTCTGATTTGTGGGAACAGACCTTGTAAATAACCACTGTTCACATGTGTGGCAGAATGAT AAGGGGCCCCTCCCAGGGGGTGCCCTAGAGCCAGGGAGGTGGCACAGCCAGCCCAACTCTGTGACCCAGG AAATGACCACCCAGAGGTCAGCTAGTTTGTACAAGAAATGGGCCCAACCCTTTGGGAGGGAGGCAAAACT GGCTCAGTTAGTGGCACGATGACGCATGAAGGAGATTATGTTGTGCTCTTTATTGCCAAAAATAAAAACT TTTAAAAAGACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499419 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499419 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11144 same embryo
	EMAGE:11143 same embryo
	EMAGE:11145 same embryo
	EMAGE:11141 same embryo
	EMAGE:11140 same embryo
	EurExpress:euxassay_003382 same experiment
	MGI:4824373 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source