EMAGE mouse gene expression spatial database

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EMAGE:11145

Akirin1 akirin 1 ( MGI:1915300)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003462. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003462_01	euxassay_003462_02	euxassay_003462_03	euxassay_003462_04

euxassay_003462_05	euxassay_003462_06	euxassay_003462_07	euxassay_003462_08	euxassay_003462_09

euxassay_003462_10	euxassay_003462_11	euxassay_003462_12	euxassay_003462_13	euxassay_003462_14

euxassay_003462_15	euxassay_003462_16	euxassay_003462_17	euxassay_003462_18	euxassay_003462_19

euxassay_003462_20	euxassay_003462_21	euxassay_003462_22	euxassay_003462_23	euxassay_003462_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11145_wholemount_strong.wlz

11145_wholemount_moderate.wlz

11145_wholemount_weak.wlz

11145_wholemount_possible.wlz

11145_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11145_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 17 weak expression: see section 13 14 15 16

vibrissa

moderate

regional

moderate expression: see section 07 08 weak expression: see section 09 10 11 21 22 23 24

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 10 11 17 18

facial vii ganglion

strong

regional

strong expression: see section 05 06 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 10 20

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 08 09 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 12 13 19

cervical ganglion

moderate

regional

moderate expression: see section 11 19

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 13 14 16 17 18 19 moderate expression: see section 12 20

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 16 17 18 19 20

vomeronasal organ

moderate

regional

moderate expression: see section 06 07 08 09 21 22

esophagus

moderate

regional

moderate expression: see section 15

meckel's cartilage

moderate

regional

moderate expression: see section 04 05 06 10 11 weak expression: see section 07 09 19 20 21 22 23 24

lower jaw incisor

weak

regional

weak expression: see section 13 14 17 18

lower jaw molar

moderate

regional

moderate expression: see section 10 11 weak expression: see section 09 19 20 21 22 23

upper jaw incisor

weak

regional

weak expression: see section 13 14 17 18

upper jaw molar

moderate

regional

moderate expression: see section 10 weak expression: see section 09 19 20 21 22 23

left lung

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14

right lung

strong

regional

strong expression: see section 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 22 23 24

clavicle

moderate

regional

moderate expression: see section 10 11 12 13 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6208
Entity Detected:	Akirin1, akirin 1 ( MGI:1915300)
Sequence:	sense strand is shown >T6208 TTCTTCCCCTCTGCCGGGCGGTGGCGCACGCCCGTGACGTCACAGGAGGCGGGGCCAGCGCGGCTGCCGG GTGCCGGAGGCGCCATTGGAGCCGGCTTGGCTTGGGAGCCGTAGCTGAAGAGTTGGATCCAGGGCAGTCC TTGCTGCCGCGTCTGCGTCTGGTCTCTGGCCCTTCCGCGGGATGGCGTGCGGGGCGACACTGAAGCGGCC CATGGAGTTCGAGGCGGCGCTGCTGAGCCCCGGTTCCCCGAAGCGGCGACGCTGCGCCCCTCTGCCCGGC CCCACTCCGGGCCTCAGGCCCCCGGACGCCGAGCCGCCGCCGCTTCAGATGCAGACGCCGCCGGCGAGCC TCCAGCAGCCTGCCCCACCCGGCAGCGAGCGTCGTCTTCCAACTCCCGAGCAAATTTTTCAGAACATAAA ACAAGAATATAATCGTTACCAGAGATGGAGACATTTAGAAGTTGTTCTCAGTCAAAGCGAAGCCTGTACT TCAGAAACTCAGCCTTCCTCCTCGGCACTTACTGCACCAGGTTCTCCAGGTGCTTTCTGGATGAAGAAGG ACCAGCCCACCTTTACCCTTCGACAAGTGGGGATAATATGTGAGCGTCTCTTAAAAGACTATGAAGATAA AGTCCGAGAGGAGTATGAGCAAATCCTCAGTACCAAACTCGCAGAACAGTATGAATCGTTTGTGAAATTC ACGCATGATCAGATCATGCGGCGATACGGGACAAGGCCAACAAGCTATGTATCCTGAAGCCTTCTTGCCT ATCTGGGTACCAGGTGTGACCTCAAGAGATGGCTGCTGTACACTTTTTGCAACTGGTTTGATATCACATT TCAGCTCCAACTTTGCATCCTGAGAACACTTAAATGTTTCTGCAGGTCCATTTTATACAACTTGAAAGAC CTTAAAACTTTCTGGTTGCCACAAGCATATCTTTCTTTTCTGCTCATCCACTAAACAGCTGTGCCCTACT GTGATAGGTTTTCCAGACAAAGTACCTGGAACAGCAGTCTAGCAAAGAATGCCTTCAGTGGCACTCAACA CGTGGAGTCTCCCCAAGCACAGCTCTGTGAGAAGTGTGAGTGTGTGCGTGTGTGTGTCTGTGTTTTAAGT TATTATTTGTATTGTGCAAAAAAAAAATTTTGATCTTGGGGATTCTGGCTGTGAATTTGATGCACGACAA TTATGGTTAAAAACATTTTCTTGGTCTACAGAAGATCATTAATGTTTGGTGACCATATAGGTTGTAACAG TGGGTTGTTTATTTGTAGGTATTATCGTTAAATACAGGGACTGTTTCCAGGCACAGAATTTGAAATGTAA GTTAAGATGGACATTAGATGTGATTATGATGATCTAGGGAAGGTCTGCAGTCCTGAATCTGCAAATACGT GGTGAGAAATTAGAACAAACTGGAGACAGGCTATCAGTACATGGACTCTGCCTAGCTGTGCTGGAAAAAT ACTTTGACTCCAAGCCTTAAAATATCCTGTGGAGTCTGAGCTCACCTCATTCATACAGTCACGGAGCTCC CTGAACGCTGAACTTCTACAGGGAAAAGATCTCCCCTGGAGCGGAAGATCAGGGTGTCCTCGGAGCGTGT AACAAGTGCGGGGCTGGGCCAGGCTCACAGCACTGCTACCTGGGAGGAGCCGCTCACCTTCGCATCAGTT CCAACATAGAATCTGGACCCTTTGTCCAGGTTACCCCAAACTCTCCTGAGGTGTCCGTGTTCAACCCTTA GAGCTTGGTTTTGGCAATACCCTGCCTGAAGTTGCTTATTGGCTGTTCTCTGCCTTGGTCCCAAGGCTGT GAGCAGAAAGCAGCCTCTGTTTTGAGGAGGTGGGATTTAAGTATACATTTATTTTTACTGTGACTTGTTC AGGACCACATTTTACAAAATGCCTTGTTTCCTTTACTGTTGTTTTGGAAAGGAAAGTTTTATGAAAACTG TTTTAGTTTGAATATAGAATAGTTATATTAATTACGGCTTTATTTTGGAAAAATCTTGAGTTTTAATTCA AATGTTTGCTAATACCTTCCAAAGTAAGGTGATATTCAGAGACAGTTGTTGTGATCAGCTGGCTTGCGGA CATTTCTGGGGTATTCACATTTGAAGATTCCTTATTAATGAATGTCTTTGACTTAAATCTAACCAAAAAC TGCAACATTATTCTTTGTACATTTTCATTATATAGTGTTAACAAGCTTAGTTGCAAACGAATAAAATACT TAAGCTATTTGTTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498569 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498569 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11142 same embryo
	EMAGE:11144 same embryo
	EMAGE:11143 same embryo
	EMAGE:11141 same embryo
	EMAGE:11140 same embryo
	EurExpress:euxassay_003462 same experiment
	MGI:4823065 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source