EMAGE mouse gene expression spatial database

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EMAGE:11325

Dcp1a DCP1 decapping enzyme homolog A (S. cerevisiae) ( MGI:1923151)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006579. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006579_01	euxassay_006579_02	euxassay_006579_03	euxassay_006579_04

euxassay_006579_05	euxassay_006579_06	euxassay_006579_07	euxassay_006579_08	euxassay_006579_09

euxassay_006579_10	euxassay_006579_11	euxassay_006579_12	euxassay_006579_13	euxassay_006579_14

euxassay_006579_15	euxassay_006579_16	euxassay_006579_17	euxassay_006579_18	euxassay_006579_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11325_wholemount_strong.wlz

11325_wholemount_moderate.wlz

11325_wholemount_weak.wlz

11325_wholemount_possible.wlz

11325_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11325_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8118
Entity Detected:	Dcp1a, DCP1 decapping enzyme homolog A (S. cerevisiae) ( MGI:1923151)
Sequence:	sense strand is shown >T8118 CTTTGCCTGGATGGCACTTTCCTGTTCCACAGTCCGCCCCCGCCGGAGGGGCTCGGCTCTGAGATCCAAG ATGGAGTTGTTGAGTCGAGCTGAGCAAGAGATGAGTCTGGCGGCCTTGAAACAACACGACCCCTATATCA CCAGCATCGCAGACCTCACGGGCCAGGTCGCCCTGTACACTTTCTGCCCCAAAGCCAACCAGTGGGAGAA GACTGATATAGAAGGGACGTTATTTGTATACCGAAGGTCAGCTTCGCCTTACCATGGCTTTACCATTGTC AACCGACTGAACATGCACAATCTGGTTGAGCCAGTGAATAAAGATTTGGAATTTCAGCTCCATGAACCAT TTCTTCTGTATAGAAATGCAAGTTTGTCAATATACAGTATCTGGTTCTATGACAAGAATGACTGTCATCG CATAGCAAAACTCATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAGGCTGCTCGAGATAAA CAGAGTCCCAGCCAGGCCAATGGCTGCAGCGACCAGAGGCCCATAGATATCCTGGAGATGCTGAGCCGGG CCAAGGATGAGTATGAGAGGAATCAGATGGGCGGCTCAAATATCTCCAGTCCTGGGCTACAGCCAAGCAC TCAGCTCTCTAATCTGGGAAGCACCGAGACTCTAGAAGAGACACCCTCTGGGTCACAGGATAAGTCTGCT CCGTCTGGTCATAAACATCTGACAGTAGAAGAGTTATTTGGAACCTCCTTGCCAAAGGAACAACCAACAG CTATGGGTCTAGAGTCAGAGGACACAGACAAGCTGCTGGGAGATGCATCACAGAAAGAGCCCAGCTCATT CCTCCCATTTCCCTTTGAGCAGTCAGGGGGAGCCCCTCAGTCTGAAAACCTGGGTATCCATTCTGCCGCT CACCACACAGTTCAGCCTGAAGTCAGCACCCCAGTGCTGATCACTCCCGCCTCCATTGCACAGTCGGGTG ACAAACATCCCCCAAGCTACACGCTCCCACTGAGCCCTGTCCTCAGCCCCACTCTGCCAGCTGAAGCTCC TACCACACAAGTTCCCCACTTACCTCGGAATAGCACCATGATACAAGCAGTAAAGACCACACCCAGGCAG AAATCTCCACTCCTGAACCAGCCAGTCCCTGAGCTAAGCCACAGCAGTCTGGTTGCTAGCCAGAGCCCCT TCAGAGCCCCGGTGAGCCTAGCAAACCCAGCTGGCACAGCCCTCCCAAGCGTTGATCTGCTTCAGAAACT CAGGTTGACCCCACAGCATGACCAAATACAAGCACAGCCGCTTGGAAAAGGAACCATGGCGCCCAGCTTT TCTTCGGCAGCTGGCCAACTGGCCACCCCTGAGAGCTTCATAGAACCTTCTTCTAAGACAGCAGCAGCAA GAGCAGCAGTCTCAGCCTCCCTGAGCAACATGGTGCTTGCTCCAACCCTCCGGTCTATGCAGCAAAACCA GGATCCTGAAGTATTTTCCCAGCCTAAGGTGTTACCCAGTGCCATCCCGATTGCAGGCTCTCCATTGGTT CCTGCAACCACCACAGCAGTATCCTCAGTCCTGCTGTCCCCCAGTGTGTTCCAGCAAACGGTTCCCAGGG CCGCAGACCTAGAGAGGAAAGCCAGCTCCCCCTCTCCCCTCACTGTTGGGACAGCAGAGAGTCAGAGAAA GCCTTCCATTATCCTCAGCAAGTCTCAGCTCCAGGACACACTGATACACCTAATAAAGAATGACTCCAGC TTCCTCAGTACGCTTCATGCAGTTTACTTGCAGGTTCTGACCAAGAACAAAGACAACCACAACCTATGAC TGCAGTGAAGGCAATGTGAAGGGAGAGGGTCCGCCTCAAGACACGCAGGCTTCCTCATGGAACAGCTAAG CAGAGCATGGAGCTTGAGGAGCCTGAGAGCCTTGTCCGTAAGGATGTTTCCTAGAGACGTTCTCAGTGAT GATGGAGGTTTCACTGTGCAGCATCACCAGCAAACCTTTGTTTGACAAAACAACAAGAGACCATTGTGTT GAGTGACGTGGGTGTTTTCATCAGCTACAAGCCAGTGGGTGGAATGAGGAGCCTGACTGCCGGCTTGCTT TCCAGGCCTCGGGAGGAGCCACCAGCCTTGCAGCAGAGCTCAGTGGAGCCCACGCCCTCCCTCCTCTACT CCTAGAAGAACTTAATGTCAGCTCACAGAGTAACTTCAGGTTTTAAGGATCAGAATTTAAGTTGACTCTT TGGAAGGTATTTGTTCAAGTGAAGTATATTTTCTATGAGCACCCCGCTTGGGCCTTAAGACTTCTGGGAT GTCTTTGTGTTCCGTAGTAGAGGCCGTAACTGCCATTGTAACACTGATAATGCAACATGAAACTCAAGGT GCCTACCTGCTCATACAGCGGAGTCGGCCATCATACTGCAACTCAGAGGGAAGAGAAGGAAGGGCACGGG CACAGGAGGACCCTGCGAGTCAGGCACTGAGGGGTCACAGCCTGCGTCGTCCTGTGTCCTCGTGGCACAC CTACTGAGGACCATGTGTCTCTTCCTGCCTGACTTTGTTGTTCTTCAGTACCAGCATTGCCCGCCCTGTT CAAGAATCCAGAATGCTCCGGTCTGAGCAGAGCACCTTACAGTGGGCTTTGAAGGCCACTCACGAGGAGA GTTTCCATGAGGACCAGGGCCTCCAGGAGCCTGTGGGGTTTAGGTTTGGGGTTGTTTTGTTGTTTTTTTA TTTCAAGTTTCAGCAGGAGAGATGTGCAATGTTTCAATAGGATTCCGATTTGACTTCACTCTTTCAAACG CTGCAATGCAATACAGATCACTTTGAAGTGGGGAAAAATGCAAAGTTGTGTTTCCCTTTTATCTGTGAAT CTTTAAGACACGTGTTGTTGCTGTACGTGCTGAGTGGTCTTATTCTCTACTGCTTCGGCGTGGGGCTCCC ATGACGTCATTGCACCGGGCTCTCAGCCCTTCCGGGGAGGCAAGAAGCCCCGCGCCACCTCGAGCGAAGC TTTTGTTCCGTGAGCGCAGTACCACCTCATTAACCTTTTTAGTGGTGTGGATTTTATACCATCTGTGTAT GTTTGCAAATATTAAGTTATTACATGTTTCAAAATGAGCATTTTTCATCCTAAATTTTATATGTTTGCAA ATATATTTTTTTAATAAAATTTCAAAACCAAGTTTTAGCACCTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30049516 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30049516 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11326 same embryo
	EMAGE:11328 same embryo
	EMAGE:11327 same embryo
	EurExpress:euxassay_006579 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source