EMAGE mouse gene expression spatial database

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EMAGE:11328

Cpt1c carnitine palmitoyltransferase 1c ( MGI:2446526)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006571. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006571_01	euxassay_006571_02	euxassay_006571_03	euxassay_006571_04

euxassay_006571_05	euxassay_006571_06	euxassay_006571_07	euxassay_006571_08	euxassay_006571_09

euxassay_006571_10	euxassay_006571_11	euxassay_006571_12	euxassay_006571_13	euxassay_006571_14

euxassay_006571_15	euxassay_006571_16	euxassay_006571_17	euxassay_006571_18	euxassay_006571_19

euxassay_006571_20	euxassay_006571_21	euxassay_006571_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11328_wholemount_strong.wlz

11328_wholemount_moderate.wlz

11328_wholemount_weak.wlz

11328_wholemount_possible.wlz

11328_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11328_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

weak

regional

weak expression: see section 10 11 17 18 19

brain

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 01 02 03 04 05 06 07 08 09 10

cerebral cortex marginal layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 moderate expression: see section 04

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 09 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19 20

trigeminal v nerve

weak

regional

weak expression: see section 10 17

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 weak expression: see section 10

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 16 17

cervical ganglion

moderate

regional

moderate expression: see section 09 10 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 16 17 18 19

stomach

weak

spotted

weak expression: see section 03 04 05 06 07 08 09 10 11

midgut

weak

spotted

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21

metanephros

weak

regional

weak expression: see section 10 11 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8121
Entity Detected:	Cpt1c, carnitine palmitoyltransferase 1c ( MGI:2446526)
Sequence:	sense strand is shown >T8121 GGCGCCCGCGAGGTGTCTCTGGGTTTCAGGATAACAGCCAAAGACGGTTGATCAGTTCGTGGACTCATTA CACGGGGACTGGATGTATGTACATGGGAAACTGGGTACTGGACTCAAACTGGGGGAAGGAGGTGTATATG GAATTGACCTCAGGAACGGCAGCTGCTGACCTCTGACCGGGGCCCAGGGAACATGGCTGAGGCACACCAG GCCTCGAGCTTGCTGTCTTCACTGAGTTCCGATGGGGCAGAAGTAGAGCTCAGCTCGCCAGTGTGGCAGG AAATTTACCTCTGTGCCCTGCGCTCCTGGAAAAGGCATCTCTGGCGTGTCTGGAATGACTTTCTTGCTGG TGTGGTCCCTGCAACCCCCCTCAGCTGGCTCTTCCTCTTCAGTACAATCCAGCTTGCCTGTCTCCTCCAG CTGGACCCTTCCCTAGGACTGATGGAGAAGATTAAAGAGTTACTGCCAGACTGGGGCGGACAGCACCATC AGCTCCAGGGGCTCTTGTCAGCAGCAGTTTTTGCATCTTGTTTGTGGGGAGCCCTCATCTTCACCCTTCA TGTGGCTCTGAGGCTCCTTCTGTCCCACCATGGCTGGCTTCTGGAACCTCATGGCGCCATGTCCTCACCC ACGAAGACCTGGCTGGCATTGGTCAGAATCTTTTCCGGGAGGCACCCGAGGCTCTTCAGCTTCCAACGCG CGCTCCCGCGACAGCCAGTGCCCTCGGCGCAGGAGACCGTGCGAAAGTACCTAGAGTCTGTACGCCCTGT GCTCAGAGACGACGCTTTCGACCGCGCTACAGCGCTGGCAAATGACTTCCTGAGGTTGCACGCACCACGG CTACAGCTCTATTTGCAACTCAAGTCCTGGTGTACTTCCAACTACGTCAGCGACTGGTGGGAAGAGTTTG TGTACCTGCGCTCTCGAGGCTCACTGATTAATAGCACCTACTACATGATGGATTTTCTGTACGTCACCCC CACACCGCTGCAGGCAGCCCGCGCGGGCAATGCTGTGCATACCCTGCTCCTGTATCGCCACCTGCTGAAC CGGCAGGAGATCTCACCGACATTGCTGATGGGGATGCGACCCTTGTGTTCCGCCCAGTATGAGAGGATGT TCAACACTACACGGATTCCAGGGGTGGAAAAAGACCACCTTTGTCACCTTCAAGACAGCCGACACGTGGC TGTCTTCCACCGGGGCAGATTCTTCCGTGTAGGGACTCACTCTCCCAATGGCCTGCTTTCCCCACGGGCC CTGGAACAACAGTTCCAGGACATCCTGGATGACCCCTCCCCAGCCTGTCCCCTTGAGGAACACCTAGCTG CTCTGACTGCTGCTCCCAGGAGTATGTGGGCTCAGGTGCGGGAGTCGGTGAAGACCCACGCAGCCACCGC CCTGGAGGCTGTGGAAGGGGCTGCCTTCTTTGTGTCCCTCGATTCTGAGCCCGCGGGACTGACCAGAGAG GACCCCGCAGCTTCCCTGGACGCTTATGCCCACGCTCTGCTGGCTGGCCGAGGCCATGACCGCTGGTTTG ACAAATCCTTCACCCTCATCGTCTTCTCCAATGGGAAGCTGGGCCTCAGCGTGGAACACTCATGGGCCGA CTGCCCTGTCTCAGGGCATTTGTGGGAGTTCACCCTGGCCACAGAGTGCTTCCAGTTGGGTTATGCCACA GATGGCCACTGTAAGGGGCACCCTGACCCTACCCTGCCACAGCCCCAGCGCCTGCAGTGGGACCTTCCAG AGCAGATCCAGCCGTCCATCTCGCTGGCCCTGAGGGGAGCCAAGACCTTGTCTGGCAACATCGACTGCCA CGTCTTCCCCTTCTCCCACTTCGGCAAGAGCTTCATCAAATGCTGTCAAGTCTCTTCAGACAGCTTCATC CAGTTGGTCCTGCAGCTGGCCCACTTCCGGGACAGGGGTCAATTCTGCCTGACTTATGAGTCAGCCATGA CCCGACTGTTCCTGGAAGGCAGGACAGAGACAGTGAGGTCTTGCACCAGAGAAGCCTGCCAGTTTGTGAG AGCCATGGACAACAAGGAGACAGACCAACATTGCCTCGCCCTGTTCCGAGTGGCCGTGGACAAGCACCAG GCTCTACTGAAGGCGGCGATGAGTGGCCAGGGGATTGACCGTCACCTCTTTGCACTGTACATCATGTCCC GGCTCCTCCATATGCAGTCGCCCTTCCTGACCCAGGTCCAGTCGCAGCAATGGCTGCTGTCCACCAGCCA GGTCCCTGTGCAGCAGACACACCTGATTGATGTCCACAATTATCCGGATTACGTTTCCTCTGGAGGTGGA TTTGGGCCTGCCCATGACCATGGGTATGGTATCTCCTACATCTTCATGGGGGAAAACGCAATCACCTTCC ATATCTCCAGCAAGAAATCAAGCACAGAAACAGACTCTCACCGGCTGGGGCAGCACATCGAAAATGCCCT GTTGGATGTAGCCTCCCTGTTCCGGGTTGGACAGCATTTCAAGCGCCAGTTCAGGGGGGAGAATTCGGAT TATAGATACAACTTTCTCTCCTGTAAGACTGTGGACCCCAATACCCCTACATCCTCCACCAACTTGTGAG CCCCTCAGCAGGGAGCTGGTCTCCCTCAGGATGGAAGGTGTTGCTCCCAGGGCTTGGCTGGTGCAGACAG GGGTATCGGGGATTCCCACACCCACCTGGTAATAAAGATGTGAGTTGGGTGCAGTAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:30089333 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30089333 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11326 same embryo
	EMAGE:11327 same embryo
	EMAGE:11325 same embryo
	EurExpress:euxassay_006571 same experiment
	MGI:4824037 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source