EMAGE mouse gene expression spatial database

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EMAGE:11327

Man2b2 mannosidase 2, alpha B2 ( MGI:1195262)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006578. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006578_01	euxassay_006578_02	euxassay_006578_03	euxassay_006578_04

euxassay_006578_05	euxassay_006578_06	euxassay_006578_07	euxassay_006578_08	euxassay_006578_09

euxassay_006578_10	euxassay_006578_11	euxassay_006578_12	euxassay_006578_13	euxassay_006578_14

euxassay_006578_15	euxassay_006578_16	euxassay_006578_17	euxassay_006578_18	euxassay_006578_19

euxassay_006578_20	euxassay_006578_21	euxassay_006578_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11327_wholemount_strong.wlz

11327_wholemount_moderate.wlz

11327_wholemount_weak.wlz

11327_wholemount_possible.wlz

11327_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11327_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 weak expression: see section 13

choroid invagination

moderate

regional

moderate expression: see section 15 16 weak expression: see section 06 07 08 09 17 18 19

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 17 18 19

pons ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 06 07 08 09 17 18 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 08 09 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8116
Entity Detected:	Man2b2, mannosidase 2, alpha B2 ( MGI:1195262)
Sequence:	sense strand is shown >T8116 CCTGGCAGCGTCCCACTGCCCAGCAATGGGGCCGCTGCGCTGGCTGCCGCTACTCGGCCAGCTGTTGCTG TTGTGGCCGCGGGCAGCTCAGCCCGCCGGCCCGATCCGGGCCTTCGTGGTGCCCCACAGTCACATGGACG TGGGCTGGGTCTTCACGGTCCAGGAAAGCATGAGAGCCTATGCAGCCAATGTCTACACTACCGTGGTGGC GGAACTGGTCCGCGGAGGGCAGCGGCGCTTCATCGCCGTGGAGCAGGAGTTCTTCCGGCTGTGGTGGGAC GGTGTGGCCTCTGAGCAGCAGAAACAGCAGGTCCGCCAGCTCCTGCATGAAGGACGCCTGGAGTTTGTTC TCGGAGGCCAAGTCATGCATGATGAGGCTGTGACCCACCTGGATGACCAGATCTTACAGCTCACAGAAGG ACATGGCTTTCTCTATGAAACGTTTGGAATCCGGCCACAGTTCTCCTGGCACGTGGACCCTTTCGGTGCA TCAGCAACGACCCCTACCCTGTTTGCCCTGGCAGGCTTCAATGCCCATCTCATCTCTCGTATTGACTACG ACCTCAAGGATGCCATGCAGGAAGCTCAGATGCTGCAGTTCGTGTGGCATGGGTCCCCATCCCTGTCAGG GCAGCAGGAAATCTTCACTCACGTCATGGACCATTATAGCTACTGCACCCCCTCACACATCCCCTTTTCT AACAGGTCTGGATTTTACTGGAATGGTGTGGCCGTCTTCCCAGAGCCACCCCCAGACGGCGTGTATCCTA ACATGAGCGAGCCTGTCACCGGAGCCAACATCCACCTCTACGCTGAGGCTCTGGTGGCCAACGTTAAACA GAGGGCTGCGTGGTTCCGGACGCCCCACGTCCTCTGGCCCTGGGGATGTGACAAGCAGTTCTTCAATGCC TCGGTGCAGTTTGACAACATGGATCCCCTGCTGGACTACATCAACCAGCGCACGGCCCAGTTTGGCATCT CGGTGCAGTACGCCACACTGAACGACTACTTCCAAGCCCTGCACGCCACCAACATGACCTGGGGCATCCG TGACCACCAGGACTTCCTTCCCTATTCTTCAGAACCACTTCAGGCCTGGACCGGCTTCTACACATCCCGG AGCACCCTCAAGGGTCTAGCGAGACAAGCCAGTGCCTTGCTCTATGCTGGGGAGTCCATGTTCACACGCT ACATGTGGCCAGACCCCAGTGGGACTCTGGACCCTACCTGGGCCCTGCAGCAGCTCCAACAGCTTCGCTG GGCTGTGTCTGAGGTCCAGCACCACGATGCCATCACAGGCACTGAGTCCCCCAAGGTGAAGAACATGTAC ACGGAACATCTCAGGATGGGGATGCTGGGCGTGCGCAAGCTGATGGTCTCCATTGCCCTGGGCGGGCCCC CAGGGTCTGGCACAGGAGCCCCCAAAGACATCATGGGGCCTCAAGTGACACCTGTCCTCTCGGTAGACAC AAGGCCTGTAGGATACTCCGCCTCTGTGTACAACCCGCTGGCCTGGAAGATCACCACCATTATCACCCTG ACTGTCGCTTTCCCCAATGTCAGCGTCACCGATGAGTTGGGCCACCCGGTGTCAACACAGATTCAGAACT CTACAAAGGACCCTTCTGCATACGACCTGCTCATCCTGACCACCATCCCCGGCCTCAACTATCGGCACTA TCAAGTCATGCACGCCCGCGGGGACCAGGCAGGCACCAGGGAGCTGGTCGCCCCCAGGGCCAACACCCTC AAGTTTAGTCTCAAACTGAGGAACCAGCCCAGCCAGGAAGGCAAGCGCCTGGTGCCCGTGATGAATGACT GCTATATTCTGCTGTTCGACCAGGACACCAACATGTTACATAGCATTCAGGATAGACAGAGCAACCGCAC GGTGCGCATGACCCAAGAGTTCCTGGAGTACCAGGCCAACTGGGACGTGAAGCAGGGCCCCATTTCTGAC AACTACCTCTTTGCACCCAACAACACCGCTGAGCCTTCCTGGGAAGCGGTGGGGATGGAAATGGTGGCAG GCACACTGGTAACGGACATAAGGCAGTATTTCTACAGGTACATAACAGACCAGGAGTACATCTATTCCAT CCACACCCGTCTGGCCCACCCGAGCCTCGCTGGAGAACTGCTCTGCCAACGGATAGAGCAGCAGTACCGA GTGGGCCCCCTGGACCTAAACCGTGAGGCCATCCTGAGGACCAGCAGCGACCTAAACAGCCAGCAAGTCC TCTACTCTGACAACAACGGCTACCAGATGCAGCGGAGGCCCTACAAGGCTTTCAAGAGCAATCCCATCCC CCGGAATTACTACCCCATGGTACAGTCAGCCTTCATCGAGGATGACAAGAGCAGGCTGGTGCTGCTGGCA GAGCGACCGCACGGTGTCTCCAGCCAAGGGAATGGGCAGGTGGAGGTGATGCTCCATCGACGGCTGTGGA ACAACCTGGCCTGGGACCTGAAGTACAACCTCACGCTGAACGACACCTCCATTGTCCACCCGGTGCTCTG GCTCATGCTGGGGCCCAAGTCCACCATGACGGCCCTGCACCCAAGGAGTGGGGTGGCTCTGCAGCACGGG CCCGTTGTGTTGTTAAAAGAACTGGCTGACGAAGAAACGCCCGTTCACGGACCCCACAATCCCTGGCCTG TGACACTGCCCCCAAACCTGCATCTGCAGATCCTCAGCGTACCTGGCTGGACGTACAGCAGGAGCCATGC CCAGCACCTGAGGAACCTTCAGAGAGGCCACCCGGAGAAGCCACAGGCCAACCTGCAAAGGGTGCTGCTG CGCCTGCGCCACCTGTATGAAGCAGGGGAGGACCCAGTACTGTCTCGGCCTGCGACAGTGGATCTCAAGG TTGTACTTCGAGGACTAGGGTCTGTAGTGGCTGTGGAGGAACGCTCACTCACAGGGACCTGGGATGTGCA GATGCTGCAGCGCTGGCACTGGAGCACAAAGACTGACCACCTGAAAGGCCACCCCACCTCTCCACCAAGG CCACCAGGAGGCTCCATCATCACCGTGTACCCTAAGGAAATCCGGACCTTCTTCATTAAATTCCAGCAGT GACATCAATGCATTGGCCCCAGGGACATGGAACTGGAAACCCCACCCACCCCACCCCCAAAAAAAACAAC GACTTCAGACCCAGAGGGAGGAACAAGCTGCGCATCAGACAGCTGTGTGGCAGAAAGTGGCCATGGGGGA GAGGGATAGCTCTTATTATTTTTTTTTTTTTTAATAAAAATTAAGGTCTGGGCTCTTTCTTCCCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30101816 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30101816 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11326 same embryo
	EMAGE:11328 same embryo
	EMAGE:11325 same embryo
	EurExpress:euxassay_006578 same experiment
	MGI:4826074 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source