EMAGE mouse gene expression spatial database

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EMAGE:11337

Slc17a9 solute carrier family 17, member 9 ( MGI:1919107)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003504. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003504_01	euxassay_003504_02	euxassay_003504_03	euxassay_003504_04

euxassay_003504_05	euxassay_003504_06	euxassay_003504_07	euxassay_003504_08	euxassay_003504_09

euxassay_003504_10	euxassay_003504_11	euxassay_003504_12	euxassay_003504_13	euxassay_003504_14

euxassay_003504_15	euxassay_003504_16	euxassay_003504_17	euxassay_003504_18	euxassay_003504_19

euxassay_003504_20	euxassay_003504_21	euxassay_003504_22	euxassay_003504_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11337_wholemount_strong.wlz

11337_wholemount_moderate.wlz

11337_wholemount_weak.wlz

11337_wholemount_possible.wlz

11337_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11337_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 15 16

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7397
Entity Detected:	Slc17a9, solute carrier family 17, member 9 ( MGI:1919107)
Sequence:	sense strand is shown >T7397 GCCTGATGCAGCCAATCCCAGAGGAGACCCGCAAGACCCCTTCTGCGGCAGCAGAGGATACACGGTGGTC CAGGCCTGAATGCCAGGCCTGGACAGGAATCCTGCTCCTGGGCACCTGCCTGCTGTACTGCGCCCGAGTC ACCATGCCCGTCTGTACTGTTGCCATGAGCCAGGACTTTGGCTGGAACAAGAAGGAGGCTGGTATCGTGC TCAGCAGCTTCTTCTGGGGCTACTGCCTGACTCAGGTGGTGGGCGGCCACCTTGGGGATCGGTAACCAAC CCCCTCTCCCCACTGCTCAGGCCTGAGGGGGATCATTACCATCACCTGACACAGTGTGGTCAGTCCCGCC CTTGCACACAGGAGAGGAGGGGTGGATAGGGGAGGAGTTCAACAAATATTTGGGGAGAGGCTTAGCCTTC GGGGAGAGGAAGTCGGGTGTGGCAGTGCTGAGCCACAGCAGACAGGCCAAACTCAGTCCTGTATACCATA CCTCAGCTCTGGGCCCTTCTATAGGGCAGGTCACTGCAGTGTCTACCACAGCTCTCTGTGCATTGGTTCA CAGTGATGACCCTCAGACCCCCCTCAAACAGCCACCCCATCCTGTCCCTCCCTGTGTCTCCTCCAGCATC GGAGGAGAGAAGGTCATCTTGCTGTCAGCCTCCGCCTGGGGCTTCATTACTGTCACCACACCACTGCTTG CGCACCTCGGCAGCGGCCACCTGGCCTTCCTGACATTCTCTCGAATCCTCACCGGTCTGCTCCAAGGTGT CTACTTTCCAGCCCTGACCAGTCTGCTGTCCCAGAAAGTACAGGAGAGCGAGAGAGCCTTTACCTACAGC ACTGTGGGTGCTGGCTCCCAGGTCGGTGTTTTATCTGCTGTTGCTGCTGTGACAAAACAATGACAAGAAA CAGATTAAGAAGAAAGGATTTGTCAGGTGATGATAGTGCACACCTTGAATCTCAGCACTCGGGAGGCAGA GGCAGGTGGATCTTTGTGATTTCGAAGCCAACCTGGTTCCAGGTGAGTGAATTCCAGGATAGCCAGGGCT ATACAGAAAAACCCTGTCTCAGAAGAAGGGAAGGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA AGAAGAAGAAGAAGAAGAAGAAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4194357 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4194357 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11339 same embryo
	EMAGE:11342 same embryo
	EMAGE:11338 same embryo
	EMAGE:11340 same embryo
	EMAGE:11341 same embryo
	EurExpress:euxassay_003504 same experiment
	MGI:4828121 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source