EMAGE mouse gene expression spatial database

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EMAGE:11339

F11 coagulation factor XI ( MGI:99481)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003510. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003510_01	euxassay_003510_02	euxassay_003510_03	euxassay_003510_04

euxassay_003510_05	euxassay_003510_06	euxassay_003510_07	euxassay_003510_08	euxassay_003510_09

euxassay_003510_10	euxassay_003510_11	euxassay_003510_12	euxassay_003510_13	euxassay_003510_14

euxassay_003510_15	euxassay_003510_16	euxassay_003510_17	euxassay_003510_18	euxassay_003510_19

euxassay_003510_20	euxassay_003510_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11339_wholemount_strong.wlz

11339_wholemount_moderate.wlz

11339_wholemount_weak.wlz

11339_wholemount_possible.wlz

11339_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11339_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7396
Entity Detected:	F11, coagulation factor XI ( MGI:99481)
Sequence:	sense strand is shown >T7396 GGACAAGACTATAGGAAAGAAAGCAAACACTCCGCCGAGGACTACAGCAAAGACAGAAAGTATCTGCAGG ATGACCTCATTACATCAGGTGTTATATTTTATCTTTTTTGCCTCAGTTTCTAGTGAATGCGTTACTAAGG TCTTCAAAGACATCAGCTTTCAAGGAGGTGACCTGAGTACTGTTTTCACACCGAGCGCCACATACTGCCG CTTGGTCTGCACTCACCACCCACGGTGCTTGCTCTTCACGTTCATGGCTGAGTCATCTTCGGATGATCCT ACCAAATGGTTTGCCTGCATCCTGAAGGACAGCGTCACAGAAATATTGCCAATGGTAAACATGACAGGCG CGATCTCTGGATATTCCTTCAAGCAATGCCCTCAGCAATTAAGTACTTGCAGCAAAGATGTGTACGTGAA CCTAGACATGAAGGGCATGAACTATAACAGCTCTGTCGTGAAGAATGCTCGAGAATGCCAGGAGAGATGC ACAGACGATGCCCACTGCCAGTTTTTCACATACGCAACAGGGTATTTTCCCAGTGTGGACCATCGTAAAA TGTGTCTTTTGAAGTACACCCGAACGGGGACGCCAACCACAATAACGAAGCTCAATGGCGTGGTATCTGG ATTTTCACTGAAGTCCTGTGGACTTTCAAACTTGGCTTGTATCAGGGACATTTTCCCTAACACGGTGCTG GCAGACCTTAACATTGACAGCGTGGTGGCCCCAGATGCTTTTGTCTGTCGTCGCATCTGCACGCATCACC CCACTTGTTTGTTCTTCACATTCTTTTCCCAAGCATGGCCGAAAGAATCTCAGAGACATCTTTGTCTCCT TAAAACCTCTGAAAGTGGATTACCAAGCACACGCATTACAAAGAGCCACGCCCTTTCGGGCTTCAGTCTC CAGCACTGCAGGCACAGTGTCCCAGTATTCTGCCATCCGTCCTTTTACAACGACACTGATTTCTTGGGAG AAGAGCTGGACATCGTCGATGTGAAAGGCCAAGAAACCTGTCAGAAAACGTGTACCAATAACGCCCGCTG CCAGTTCTTTACCTACTATCCATCGCACAGACTGTGCAATGAGAGGAACCGCAGGGGCAGATGTTACCTA AAGCTTTCCTCCAATGGATCTCCAACGAGAATACTTCATGGGAGGGGAGGCATCTCTGGATACTCACTGA GGCTGTGCAAAATGGATAATGTGTGCACAACTAAAATCAACCCCAGAGTGGTAGGAGGAGCTGCGTCTGT TCACGGTGAGTGGCCATGGCAGGTGACTCTGCACATCAGCCAGGGACACCTGTGTGGAGGCTCCATCATT GGAAACCAATGGATACTGACAGCAGCTCATTGTTTCTCTGGGATAGAGACACCTAAAAAGCTGCGTGTCT ACGGTGGCATTGTAAATCAATCAGAAATAAATGAAGGGACTGCTTTCTTCAGGGTTCAAGAAATGATAAT TCATGATCAGTATACGACAGCAGAAAGTGGGTATGATATTGCCCTGTTAAAACTGGAATCAGCCATGAAT TACACAGATTTTCAGCGGCCAATATGCCTGCCTTCCAAAGGAGATAGAAACGCAGTGCACACAGAATGCT GGGTGACTGGATGGGGGTACACAGCACTAAGAGGTGAAGTACAAAGTACTCTTCAGAAAGCCAAGGTTCC ATTGGTGTCAAATGAAGAATGTCAGACAAGATACAGAAGACACAAAATAACCAATAAGATGATCTGTGCA GGCTACAAAGAAGGAGGGAAGGATACGTGCAAGGGAGATTCTGGAGGGCCCCTGTCCTGCAAATACAATG GGGTCTGGCACTTGGTGGGCATCACAAGCTGGGGTGAAGGCTGTGGTCAGAAGGAGAGACCGGGGGTCTA CACGAACGTGGCCAAGTACGTGGACTGGATTCTGGAGAAAACTCAAACAGTCTGAAAGAGTTCAACTGGT ATCACTTTGTGGCCCTGGAAGATTATTCCATAGAAATGAGCTTGACGTCTCTGATGAAGACACTGGGATA CTGACTCTTCCACTGTAACCAATTGAATGGCCTTGATGTACGTAAGAACACCCAGAAAGAAAACTATTAT TTTCAGAATTCCTGATCTGGGAGAACCACTGGTTGTTTTCTGCATCCAGCTACTACTCAAGGAAACAAAT ACAGCAAGGAGATTTTAAAAATAAAAACACATCAGATATATAAGGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4194672 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4194672 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11337 same embryo
	EMAGE:11342 same embryo
	EMAGE:11338 same embryo
	EMAGE:11340 same embryo
	EMAGE:11341 same embryo
	EurExpress:euxassay_003510 same experiment
	MGI:4824656 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source