EMAGE mouse gene expression spatial database

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EMAGE:11341

Zfp62 zinc finger protein 62 ( MGI:99662)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003615. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003615_01	euxassay_003615_02	euxassay_003615_03	euxassay_003615_04

euxassay_003615_05	euxassay_003615_06	euxassay_003615_07	euxassay_003615_08	euxassay_003615_09

euxassay_003615_10	euxassay_003615_11	euxassay_003615_12	euxassay_003615_13	euxassay_003615_14

euxassay_003615_15	euxassay_003615_16	euxassay_003615_17	euxassay_003615_18	euxassay_003615_19

euxassay_003615_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11341_wholemount_strong.wlz

11341_wholemount_moderate.wlz

11341_wholemount_weak.wlz

11341_wholemount_possible.wlz

11341_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11341_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6183
Entity Detected:	Zfp62, zinc finger protein 62 ( MGI:99662)
Sequence:	sense strand is shown >T6183 TGTGTGTGTGTGTGTGTGTGTGTTTGAATACGTAATGCTCACGTGTCCAAGGCATTAAAATACAAAGATT TTTAAAAAACAAAAATTTAATGCAAAATATGAAAACAAGATTTTCTTTTACTGTTCTCAAAGGTTGTTCT GGGCCCTACCTTAAAAGGAAGTTGAAATGGAAGGTCTAAAATTGAAAAGTAAATGGATGGAAAATCTGAT ATATGTGGACATGGCAGAGTATAGATAAGGTGAAGAAACTGCTGTACAAAGAAGTTAAATAGCTATCCCA AAGGTCATATAATGTCATATTTGAAGACAACCATGGAGGATGAGGAGTCGTCTAAAAAGAATGAAAATGA TTCAAATGCAGACTCTCAGTGCCCAAGTGTGGGATTTTTTCATAAGGATCATATGCAGAAATCTAAGACC GGTGACACTTGTGACTTGTTTCCTAAATGGAAAATACTGAAGGAAGGCAAAAGTAGTATCAGAGAAATGA TTGACACACATACAAACGCAGCAAATATAAAACTAGAGCAGGATGATGAGACATCTGAGAAAAGTTTCTA CCCAAGTACAAATACAATGCACCAGACTATCCCCACAGAACCAAATTGTACTAAACAAGGTGAGCACACA GAGAACATTAATGGAAACGTACACCCTGCTCATATTGCTGATAAGAAATTACATAAGTGTGATGAGTGTG GAAAATCCTTCAAATACAATTCTCGTCTTGTTCAACATAAAATTATGCACACTGGAGAAAAGCGCTACGA GTGTGATGACTGTAGGGGGACTTTTCGGAGTAGCTCCAGTCTTCGAGTCCACAAGCGCATCCATACTGGG GAAAAGCCCTATAAGTGTGATGAGTGTGGCAAAGCCTACATGTCCTACTCTAGCCTTATAAACCACAAAA GCACTCATTCTGGGGAGAAGAACTGCAAATGTGACGAATGTGGAAAATCCTTCAATTATAGTTCTGTTCT GGACCAGCATAAAAGGATCCACACCGGAGAGAAGCCCTATGAATGTGGTGAGTGTGGAAAGGCCTTCAGA AACAGTTCTGGTTTGAGAGTCCACAAAAGGATCCACACAGGGGAGAAGCCCTATGAATGTGATACCTGTG GGAAAACCTTCAGTAATAGTTCTGGCCTGAGGGTCCACAAAAGGATCCATACAGGTGAGAAACCCTATGA ATGTGATGAATGTGGGAAGGCATTCATTACTTGCAGAACACTTCTAAATCATAAAAGCATCCACTTTGGT GATAAACCTTATAAATGTGATGAGTGTGAAAAATCTTTTAATTACAGCTCTCTCCTCATTCAGCATAAAG TCATCCACACTGGTGAGAAACCTTATGAATGTGATGAATGTGGGAAGGCCTTCAGGAACAGCTCAGGCCT TATAGTGCATAAAAGGATCCACACAGGAGAGAAACCTTACAAGTGTGATATCTGTGGCAAAGCATTCAGC TATAGTTCAGGCCTTGCAGTCCATAAAAGCATTCATCCTGGAAAGAAAGCCCATGAATGTAAAGACTGTG GCAAGTCCTTTAGTTATAACTCACTTCTTCTTCAGCATAAGACCATTCATACTGGAGAAAGACCTTATGT ATGTGATGTGTGTGGCAAAACGTTCAGGAACAATTCAGGTCTCAAAGTTCATCGGAGACTCCATACTGGG GAAAAACCATATAAGTGTGATGTATGTGGGAAGGCCTATATCTCACGCTCAAGCCTTAAAAATCACAAAG GAATCCACATGGGGGAGAAGCCCTATAAATGCAGCTATTGTGAGAAATCTTTCAACTACAGTTCTGCCCT TGAACAACATAAAAGGATTCATACAAGGGAGAAGCCCTTTGGGTGTGATGAGTGTGGAAAAGCCTTCAGA AACAATTCAGGCCTTAAAGTACATAAGCGAATTCACACAGGGGAGAGACCTTACAAATGTGAAGAATGTG GAAAAGCCTACATCTCACTCTCAAGCCTTATAAATCATAAAAGTGTACACCCTGGGGAAAAGCCCTTTAA GTGTGATGAGTGTGAGAAAGCCTTCATCACATACAGAACACTTCTAAACCACAAAAAAATTCATCTTGGA GAAAAGCCCTATAAATGCGATGTGTGTGAGAAATCTTTTAACTACACCTCACTCCTTTCTCAACACAAAA GAGTTCATACTAGAGAGAAACCCTTTGAATGTGACAGGTGTGAGAAGGTCTTCAGAAACAACTCAAGCCT TAAAGTGCATAAAAGAATACACACTGGGGAGAAGCCCTATGAGTGTGACATATGTGGAAAAGCCTACATC TCACACTCAAGCCTTATTAACCATAAAAGTACACACCCTGGCAAGACGTCCTATACATGTGATGAATGCG GAAAAGCATTTTTCTCAAGCAGAACTCTTATAAGCCATAAGAGAGTCCATCTTGGGGAAAAGCCCTTCAA GTGTGTTGAGTGTGGGAAGTCGTTCAGCTACAGCTCACTTCTTTCCCAACACAAGAGGATCCACACAGGG GAGAAACCTTATGTATGTGATTAGTGTGGGAAGGCATTCAGGAACAGCTCAGGCCTCACAGTACATAAAA GGATCCACACAGGAGAGAAACCCTATGGGTGTGATGAATGTGAAAAGGCATACATTTCACACTCAAGTCT TATTAATCATAAAAGTGTCCACCGTGGAAAGCAGCCCTATAATTGTGAGTGTGGGAAATCTTTCAATTAC AGATCTGTCCTTGACCAACATAAAAGAATCCACACTGGAAAAAAGCCATATCGATGTAATGATTGTGGGA AGGCATTTAATATCAGGTCAAACCTTACCAAGCACAAAAGAATCCATACAGGAGAGGAATCTTTAAATAT GGCAAATATGGAAAGTCATAGTGGCACATTCCAGAAGATGATCTACTATGAGGGAGGGAATTCTCTGGAT GGGACCAGAATGCAGATGCCTGTGTGGGAGGCAGAGCATGCCAAGTCTCAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3497847 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497847 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11337 same embryo
	EMAGE:11339 same embryo
	EMAGE:11342 same embryo
	EMAGE:11338 same embryo
	EMAGE:11340 same embryo
	EurExpress:euxassay_003615 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source