EMAGE mouse gene expression spatial database

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EMAGE:11529

Taf6l TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor ( MGI:2444957)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006761. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006761_01	euxassay_006761_02	euxassay_006761_03	euxassay_006761_04

euxassay_006761_05	euxassay_006761_06	euxassay_006761_07	euxassay_006761_08	euxassay_006761_09

euxassay_006761_10	euxassay_006761_11	euxassay_006761_12	euxassay_006761_13	euxassay_006761_14

euxassay_006761_15	euxassay_006761_16	euxassay_006761_17	euxassay_006761_18	euxassay_006761_19

euxassay_006761_20	euxassay_006761_21	euxassay_006761_22	euxassay_006761_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11529_wholemount_strong.wlz

11529_wholemount_moderate.wlz

11529_wholemount_weak.wlz

11529_wholemount_possible.wlz

11529_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11529_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8219
Entity Detected:	Taf6l, TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor ( MGI:2444957)
Sequence:	sense strand is shown >T8219 GTGGAAGCAGCCGTCCCTCCCCAGCAGGCGCCCTCCCAACCCCGGAGTCTGCAGCAAGTGTTAATGAGGG TATAGTATGTGCTGACCCTGCTGGAGGCCTGGGATGCAAAGTTCCACCGGGGCCATGTCAGAACGAGAGG AGAGGAGGTTTGTGGAGATCCCTCGGGAGTCGGTCCGGCTCATGGCAGAGAGCACAGGTCTAGAGCTGAG TGACGAGGTGGCCGCTCTGCTCGCTGAAGATGTGTGCTACCGTCTCCGAGAGGCCACACAGAATAGCTCT CAATTCATGAAACACACCAAACGAAGGAAGCTGACGGTGGAGGACTTCAACAGGGCTCTCAGGTGGAGCA GCGTGGAGGCTGTGTGTGGCTATGGGTCCCAGGAGGCATTGCCCTTGCGCCCTGCAAGGGAGGGTGATCT CTACTTCCCGGAGGATCGAGAAGTGAGCCTGGTGGAGCTGGCACTAGCCACCAACATCCCCAAGGGCTGT GCAGAGACAGCTGTCAGAGTTCATGTCTCCTATCTGGATGGCAAAGGAAACCTGGCACCTCAGGGATCAG TGCCCAGTGCAGTGTCTTCACTGACAGATGACCTGCTCAAATACTACCAGCAAGTGACACGGGCTGTACT GGGAGATGACCCACAGCTGATGAAGGTTGCCCTCCAGGACCTCCAGACAAACTCCAAGATTGCAGCACTC CTGCCTTACTTTGTTTATGTGGTCAGTGGGGTAAAATCTGTAAGCCACGACCTGGAACAACTGCACCGAC TACTGCAGGTGGCAAGGAGCCTGATACGGAACCCACACCTGTGCTTGGGACCCTATGTCCGATCCTTGGT GGGCAGCGTGCTTTACTGTGTCTTGGAGCCACTGGCCGCCTCCATCAACCCTTTGAATGACCACTGGACT CTGCGAGATGGGGCTGCCCTTCTACTCAGTCACATATTCTGGACGCATGGAGACCTTGTAAGTGGCCTGT ATCAGCAGATTCTGCTGTCCCTGCAGAAGGTCCTGACAGACCCTGTTCGGCCTCTCTGTTCTCACTATGG GGCAGTGGTGGGGCTGCATGCTCTAGGCTGGAAGGCAGTGGAGCGAGTCCTCTACCCACATCTGCCCACT TACTGGACAAACTTGCAGGCTGTGCTGGATGACTATTCCGTATCTAATGCCCAGGTCAAAGCGGACGGGC ACAAAGTGTATGGAGCCATTCTGGTGGCCGTAGAACGACTATTGAAGATGAAAGCTCAGGCAGCAGAGCC CAACCGCGGTGGCCTTGGCGGGAGAGGGTACCGTCCTGCGGAGGACCTGCCTTGGGACAGCCTTCTCCTA CAAGAATCTCCCCCCGGGGGCAGCTCCGAAACAGGTTTTGGGTCTGGTCTCCCGCCACCATCAGGGGTTG CGGGACCAGAGGACCCTTCTTCCTTGACCCTGGCAGACATCTACCGTGAGCTATACTCCTTCTTTGGTGA CAGCTTGGCCACTCGCTTTGGCACTGGCCAGCCTGCTCCCACGGCCCCGCGACCACCCGGAGACAAGAAG GAGCCTGCGGCTGCCCCGGATTCGGTGCGGAAAATGCCGCAGCTGACTGCCAGCGCCGTGGTCAGCCCTC AGGGTGACGAAAGCCCGTGCGGTGGGACTCTCGCCACCGCCACCGCCGCCTCGGAGAGCAGGCCGCTACC GCGCGTACACCGGGCGCGGGGTGCTCCTCGTCAGCAGGGCCCCGGCGCGGGAACGCGAGACGTCTTCCAG AAGAGCCGCTTCGCGCCCCGTGGCGCCCCACACTTCCGGTTTATAATCGCAGGGCGTCAGGCCGGGCGGC GCTGCCGCGGGCGCCTCTTTCAAACTGCCTTTCCTGCCCCATATGGGCCCAGCCCCGCGTCCCGCTACGT GCAGAAGCTGCCCATGATCGGCCGTACCGGCCGCCCGGCCCGCCGCTGGGCGCTCTCCGACTACTCGCTG TACCTGCCGCTGTGAGGTTGCGGCGCCTCGGAGAATAAAGCCACACCCGGAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:1244243 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1244243 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11534 same embryo
	EMAGE:11531 same embryo
	EMAGE:11530 same embryo
	EMAGE:11533 same embryo
	EMAGE:11532 same embryo
	EurExpress:euxassay_006761 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source