EMAGE mouse gene expression spatial database

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EMAGE:11534

1190005I06Rik RIKEN cDNA 1190005I06 gene ( MGI:1916168)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006730. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006730_01	euxassay_006730_02	euxassay_006730_03	euxassay_006730_04

euxassay_006730_05	euxassay_006730_06	euxassay_006730_07	euxassay_006730_08	euxassay_006730_09

euxassay_006730_10	euxassay_006730_11	euxassay_006730_12	euxassay_006730_13	euxassay_006730_14

euxassay_006730_15	euxassay_006730_16	euxassay_006730_17	euxassay_006730_18	euxassay_006730_19

euxassay_006730_20	euxassay_006730_21	euxassay_006730_22	euxassay_006730_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11534_wholemount_strong.wlz

11534_wholemount_moderate.wlz

11534_wholemount_weak.wlz

11534_wholemount_possible.wlz

11534_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11534_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 04 05 06 07 08 20 21 22 23 weak expression: see section 09 10 11 12 16 17 18 19

radius

moderate

regional

moderate expression: see section 01

humerus

moderate

regional

moderate expression: see section 01 02 03 04 05 weak expression: see section 22 23

fibula

moderate

regional

moderate expression: see section 01 02

tibia

moderate

regional

moderate expression: see section 01 02

femur

moderate

regional

moderate expression: see section 02 03 04 05 07 08 21 22 23 weak expression: see section 06

meckel's cartilage

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17 18 19 20 21 weak expression: see section 22 23

axial skeleton

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

scapula

moderate

regional

moderate expression: see section 04 05 weak expression: see section 23

acetabular region

moderate

regional

moderate expression: see section 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8221
Entity Detected:	1190005I06Rik, RIKEN cDNA 1190005I06 gene ( MGI:1916168)
Sequence:	sense strand is shown >T8221 CGCGGCTGCAGGCAGAGCAGGCCGGGCCATGACCCCCGCGGCCCACGGCTGCAAGCGCGTCGCCTGGTGT CCCTCACGGCCGCCAGCGTCCGCGCCCAGCGCGCCCCAGGAAGCGGCCCGCAGAGGCGACGCCATGGGGC TGAAGCCCTCCTGTCTGAAAGGCTTTAAGATGTGTGTCAGCAGCAGCAACAACAACCACGACGAGGCCCC CGTCCTGAATGACAAGCACCTGAGCGTGCCCAACATTATCATCACGCCCCCAACCCCGACGGGCATGGGG CTTTCCCGGGATTCTAACAAGCAAGTCTGGATGGATGAGCTGGGGTCTTATCAAGACGATGGAGAGCTGG AACCTGAAGCCTGAAGCGGCGAGACCTGTGTGGCAGCAACCTGCCCCGCTGCCCCGGGTGTTGGGGGCGT GGCTACCTGAGCAGCTGGATGGGGAACAGCGAATTGGGTCCTGGACCAAGACATGGATGGCCAGAAGAGT ACAGCAGGTGGCTGGCCTCAACGGAGCAGACATCCGTGGCCTCTGAGTCCGCACCAACCCAGACCCCGGC TTGGTGAAGAGGAGGCCACCCTGCCCTACGGTCACCGTGACCATGGACCTCGGCCCTCGAGGCCCCAGAG CACAAGGACTTCTCGCCAGAGCAGGGCCTCTGTTTTTTACAAGTTGTTTTACAGGTATGGGACCTACATC CTGAGCGAGATTTATAAATAAAGCTCCTTTGTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1245153 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1245153 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11531 same embryo
	EMAGE:11530 same embryo
	EMAGE:11533 same embryo
	EMAGE:11529 same embryo
	EMAGE:11532 same embryo
	EurExpress:euxassay_006730 same experiment
	MGI:4822602 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source