EMAGE mouse gene expression spatial database

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EMAGE:11533

Stx17 syntaxin 17 ( MGI:1914977)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006759. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006759_01	euxassay_006759_02	euxassay_006759_03	euxassay_006759_04

euxassay_006759_05	euxassay_006759_06	euxassay_006759_07	euxassay_006759_08	euxassay_006759_09

euxassay_006759_10	euxassay_006759_11	euxassay_006759_12	euxassay_006759_13	euxassay_006759_14

euxassay_006759_15	euxassay_006759_16	euxassay_006759_17	euxassay_006759_18	euxassay_006759_19

euxassay_006759_20	euxassay_006759_21	euxassay_006759_22	euxassay_006759_23	euxassay_006759_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11533_wholemount_strong.wlz

11533_wholemount_moderate.wlz

11533_wholemount_weak.wlz

11533_wholemount_possible.wlz

11533_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11533_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal cortex

moderate

regional

moderate expression: see section 10 11 12 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8218
Entity Detected:	Stx17, syntaxin 17 ( MGI:1914977)
Sequence:	sense strand is shown >T8218 CGACGGCCTCGAGCGCCTAGGCGGGAGGTGTTTCTGTTTGAGAGAAGAGAGCTGCTGCAAGAGGAAGTAG CGGCTCATCAGGCTTCAGGATGTCTGAAGATGAGGAAAAGGTGAAGTTACGCAGGCTTGAGCCAGCGATA CAGAAATTCACTAAGATCGTGATCCCAACAGACCTGGAGAGGCTAAGAAAACACCAGATAAACATCGAGA AGTATCAGCGGTGCAGAATTTGGGACAAGTTGCACGAAGAGCACATCAACGCAGGGCGCACGGTTCAGCA ACTGCGCTCCAATATCCGAGAAATGGAGAAGCTTTGCTTGAAAGTGCACAAGGATGATCTGGTCCTTTTG AAGCGAATGATAGATCCTGTCAAGGAAGCAGCAGCAACAGCTACGGCAGAGTTCCTCCAGCTCCACTTGG AATCTGTTGAAGAACTCAAGAAACAAGTCAACGATGAAGAATTACTTCAGCCTTCTCTGACCAGATCCAC AACTGTTGATGGAGTGCTCCACACTGGGGAGGCTGAAGCTGCTTCTCAGAGTCTGACTCAGATATATGCC TTGCCTGAAATTCCTCAAGACCAAAATGCTGCTGAGTCCTGGGAAACCTTAGAAGCGGACTTAATTGAGC TGAGCCATCTGGTCACTGACATGTCCCTCCTAGTGAGTTCTCAGCAGGAGAAGATTGACAGCATTGCAGA CCATGTCAACAGTGCCGCTGTGAATGTTGAAGAGGGAACCAAAAACTTGCAGAAGGCTGCAAAATACAAG CTGGCAGCTCTGCCTGTGGCAGGTGCACTCATCGGAGGAGTGGTAGGGGGTCCCATCGGCCTCCTCGCAG GCTTCAAAGTGGCAGGAATTGCAGCTGCACTTGGTGGTGGGGTGTTAGGCTTCACAGGTGGAAAATTGAT ACAAAGAAGGAAACAGAAAATGATGGAGAAGCTCACTTCCAGCTGTCCAGATCTCCCTAGCCAAAGTGAC AAAAAACGCAGTTAAAGCCCAAGCTTCCAGCTGCTGCTGTGCACCGAGCTAAGGAGCAGTTGCTGTTGCT GGAAGTGTTAGGAAGTGGCTCTCCCTGTCCATGCAGTGTTGAACCACCATGAGTGGATGTGTTTGTTAGC TGGGTGTTAATGGAGATTTTAGAGGTGGAGGAGCCTGGCCAGAGGGTGTCTGTCAGGTCAAGTTTAAAGA CTGCCAAAGAGCAAAGGTTAGGCCTGGAAATGTGAAAACTGGATCCATAACTAAGGATCTAAGAAGTGTA GACATGTAGTGGACACCTGAGGTCAGTGTATTTGTCAATTATTTCTTCCTATAGGGAGGAACAGAATTCG AGTTCAGGAGTCTCTGTCAGGTAGGGATGTTGCCGCTCAACAGGGGGCACGGGTGAACTACTTGTGGACT TGAGGGCTGTTGTACTTAGACCCATCTGTATTTTCTGGGGAAGGAAATTAACACTATAAAATAGAAAGAG AAGCCCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1243532 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1243532 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11534 same embryo
	EMAGE:11531 same embryo
	EMAGE:11530 same embryo
	EMAGE:11529 same embryo
	EMAGE:11532 same embryo
	EurExpress:euxassay_006759 same experiment
	MGI:4828524 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source