EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11648

Ahcy S-adenosylhomocysteine hydrolase ( MGI:87968)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008402_01	euxassay_008402_02	euxassay_008402_03	euxassay_008402_04	euxassay_008402_05

euxassay_008402_06	euxassay_008402_07	euxassay_008402_08	euxassay_008402_09	euxassay_008402_10

euxassay_008402_11	euxassay_008402_12	euxassay_008402_13	euxassay_008402_14	euxassay_008402_15

euxassay_008402_16	euxassay_008402_17	euxassay_008402_18	euxassay_008402_19	euxassay_008402_20

euxassay_008402_21	euxassay_008402_22	euxassay_008402_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11648_wholemount_strong.wlz

11648_wholemount_moderate.wlz

11648_wholemount_weak.wlz

11648_wholemount_possible.wlz

11648_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11648_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9181
Entity Detected:	Ahcy, S-adenosylhomocysteine hydrolase ( MGI:87968)
Sequence:	sense strand is shown >T9181 TGAGTTCATCAGTCCTAGCGGAAGCGCCGCCAGCATGTCTGATAAACTGCCCTACAAAGTCGCGGACATC GGACTGGCCGCCTGGGGACGGAAGGCTCTGGATATAGCTGAGAATGAGATGCCAGGATTGATGCGCATGC GGGAGATGTACTCAGCCTCCAAGCCACTGAAGGGTGCTCGCATTGCTGGCTGCCTGCACATGACCGTGGA GACTGCTGTTCTCATTGAGACTCTCGTGGCCCTGGGTGCTGAGGTGCGGTGGTCCAGCTGCAACATCTTC TCTACTCAGGACCATGCAGCGGCTGCCATTGCCAAGGCTGGCATTCCAGTGTTTGCCTGGAAGGGCGAGA CAGATGAGGAGTACCTGTGGTGCATTGAGCAGACGCTGCACTTCAAGGACGGACCCCTCAACATGATTCT GGATGATGGTGGTGACCTTACTAACCTCATCCACACCAAATACCCACAGCTTCTGTCAGGCATCCGAGGT ATCTCTGAGGAGACCACGACTGGGGTCCACAACCTCTACAATATGATGTCCAATGGGATACTGAAGGTGC CTGCCATCAATGTCAACGATTCTGTCACCAAGAGCAAGTTTGACAACCTCTATGGCTGCCGGGAGTCCCT CATAGATGGCATCAAACGGGCCACAGATGTGATGATTGCGGGCAAGGTGGCGGTGGTGGCAGGCTATGGT GATGTGGGCAAGGGCTGTGCCCAGGCCCTGAGGGGTTTTGGGGCCCGAGTCATCATCACCGAGATCGACC CCATCAATGCACTGCAAGCTGCCATGGAGGGCTATGAGGTAACCACTATGGACGAAGCCTGTAAGGAGGG CAACATCTTTGTCACCACCACAGGCTGTGTGGATATCATCCTTGGCCGGCACTTTGAGCAGATGAAGGAT GACGCCATTGTCTGTAACATTGGACACTTCGATGTGGAGATTGATGTGAAGTGGCTCAATGAGAACGCGG TGGAGAAAGTGAACATCAAGCCCCAGGTGGACCGCTACTGGCTAAAGAATGGGCGCCGCATCATCTTGCT GGCTGAAGGCCGTCTGGTCAACCTGGGTTGTGCCATGGGACACCCCAGCTTCGTGATGAGCAACTCCTTC ACAAACCAGGTGATGGCACAGATTGAGCTGTGGACCCACCCAGATAAATACCCTGTTGGGGTTCACTTCC TGCCTAAGAAGCTGGATGAGGCGGTGGCTGAAGCCCACCTGGGCAAGCTGAATGTGAAGCTGACCAAGCT GACTGAGAAGCAGGCCCAGTACCTGGGCATGCCCATCAACGGCCCCTTCAAGCCTGATCACTACCGCTAC TGAGTGCTGGGGCTGTCCTTCACCTTCCAGCTGCCATCCTTGTTCCGGGCCCTATCTCTCGTTCCCAAGA GCAAATGTCACCAACTTTGCAGTAGCCTGGACAGTGCTTCTCCCACACAGCCCTCCCCTTACACCCTCTG GGGCTGGTCACTCTGTCTTTGACCTCTGCTGTATCCCTCATACTGTTCCAGGTGTGGAGGGGGAATGGAG AGGTACCTGGTAGGCATCCACAGGGGACATGATCTCAGAAGTCTTGGAAGTCCTGAGGCTGGATGTTGCT AGTGATGGTCACAAGCCATGCACCTTATCAATGAGACCCTCACTTGGTCTTTAGATCTGTGTGCCTGGTT TGCAGATCCATTGGTTTCTCAGTCCAGGACCCAAGAACGAGCTCCACCAAAGAGCAGGAACCCCTGGAGT TTGAAGGCCCCCGAGAGCTGGGCCTTTTTACTGTTGGGCAGTCTCTTAAACCTCATGATACTGAGTTGGT ACTTTTTTTGGTCCCTATTTCACAAGGGTTAGGATAGATTAACCAAGAAAGGACAAGTGACAGACTGAAA GGGGCTGGAAAAATAAAGAGGAAAAGGCCTGTCACTTCTGTATAGTTGATGGTTCCTGTCACAAGCCCAG GTCACAAACAGATTAATTTGTTTTATAATGTTTATATGCTATTTAGAATGTTAACAAAGGAAGGTGGATA AAATACAGTTTCTACTGCCTAAAGAATTTTGGCTCTATTAAAATGTAAGTGTGTGGCTGGAATATGTGCA TGTATGTGGCTGACATATGTGCATGTGTGTGCAGACACTGTACCTGTGATTCTGGAGGACACCAGATGTC CCATCGCTTTCTTCCATATTCCTGAGACAGGGTCCCTCACTGGACTAGAGTGAGGCCATTTCTGCTCTTC CCAGATCCCTCACCCTAGAGCTGGGGTTAAAGTCAAGCATGGCCATGTGTAGCTTCTACAAGTGCAAACG GTCTTACCCACTGAGCCATCTCTTCACGGATCGCATAATGTGAATCACACAAAAAAGTATGAGAAATTGA ACTCCCTCTTATTTGCCCAAGTGATCAATTTACTTAACCCATTTGACCTGGAAAGCAAATAAAAACAAGC CCCGTGCACAGGATGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6828058 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6828058 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11650 same embryo
	EMAGE:11649 same embryo
	EMAGE:11652 same embryo
	EMAGE:11651 same embryo
	EMAGE:11647 same embryo
	EurExpress:euxassay_008402 same experiment
	MGI:4823037 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source