EMAGE mouse gene expression spatial database

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EMAGE:11651

Tyw1 tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) ( MGI:2141161)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008414. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008414_01	euxassay_008414_02	euxassay_008414_03	euxassay_008414_04

euxassay_008414_05	euxassay_008414_06	euxassay_008414_07	euxassay_008414_08	euxassay_008414_09

euxassay_008414_10	euxassay_008414_11	euxassay_008414_12	euxassay_008414_13	euxassay_008414_14

euxassay_008414_15	euxassay_008414_16	euxassay_008414_17	euxassay_008414_18	euxassay_008414_19

euxassay_008414_20	euxassay_008414_21	euxassay_008414_22	euxassay_008414_23	euxassay_008414_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11651_wholemount_strong.wlz

11651_wholemount_moderate.wlz

11651_wholemount_weak.wlz

11651_wholemount_possible.wlz

11651_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11651_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9191
Entity Detected:	Tyw1, tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) ( MGI:2141161)
Sequence:	sense strand is shown >T9191 TTCGCTGTCCCTCTCTCTTTGCGTTTGCCGGTTGACTTGCGTAGCTCGGGCCCCGCGGAGGATGGGTCCT CTGGATGTGTGGGACTTGTCGCCTTTGCTGTCACTGTGGATGAACAGGTTCTACATATATATGGGCTGTG CCCTGGGCCTTACCCTTTGCATCTGTGTCCAGATCATCAAGAAGCAGGTCACTCGTTCCCAGGAGAAACG TGTCCCAGGAGCTCCTGATAGCTCCCTCTCCCCTCAGAAGAAGCAGACACACGTTTCTGGAGTGAAGATT TTTTACGGATCTCAGACGGGCACTGCCAAGGGATTTGCAGTGGTTCTTGCCAAAGCTGTCACCTCTCTGG ACCTGCCTGTGGCCATTATTAATCTGAAGGAATATGATCCAGATGACAGTCTGATAGGAGAGATCACTAG TAAAACCGTCTGTGCCTTCCTGGTCGCTACGTACACGGACGGCTGCCCTACAGAGAGTGCAGAGTGGTTC TGCAAGTGGTTAGAGGAATCAGCTAATGATTTCCGCTTTGGCAAAACTTACCTGAAGGGACTTCGCTATG CAGTGTTTGGCCTGGGAGACTCTGCCTACCGGAGCCACTTCAACAAGGTCAGCACGAACGTTGACAAATG GCTCTGGATGCTCGGTGCCCAGCGGGTGCTGACCCGAGGGGAAGGCGACTGCAATGCAGTTCAGAGCAAA CATGGCAGCATCGAGGCCGACTTCACAGCCTGGAAGACCAAGTTCATCTCCCGGCTGCAGGCCCTGCAGA GAGGGGAGAAAAAGGCTTGTGGCGGGAACTGCAAGAGAGGGAAGTGTGAGTCGGCCCAGCATGGCCCAGG AGAGGCGCGGCCTCACCCGCAAGGCGAATTGCATCCTGGGGATGCTGAGGAGGAAGAGCCCTGTGAGAGC AGCAGCGAGGACGAGCTCGGGACCCAGGATTATCAGAGCCTGACTTCTGTGGTGGATGTTGAGGACCTGG GCAACATCATGAACCCCGTGAAGAGGGAAAAGAGAGAAAAGTCTCATCAGGACGGGAAAGCTGCCATGCA GAGGAACCCAGAGAAGACTGAGGACGGCGAAGGAAGAGCTATGATAACACCTGCTCTGCGGGAAGCCCTG ACTAAACAAGGGTACCAGCTGATTGGCAGCCACTCTGGTGTGAAGCTTTGCAGGTGGACAAAGTCGATGC TGCGGGGGAGAGGAGGTTGCTACAAACACACATTCTACGGCATTGAGAGCCACCGCTGCATGGAAGCCAC CCCAAGCTTGGCGTGTGCGAATAAATGTGTCTTCTGCTGGCGGCACCATACCAATCCTGTGGGCACTGAG TGGCGGTGGAAGATGGACCAGCCAGAATTGATCTTGAAGGAAGCCATTGAGAACCATCAGAACATGATTA AACAGTTCAAAGGCGTGCCAGGCCTCAAGGCGGAGCGCTTTGAAGAGGGGATGGAGGTCAAGCACTGTGC GCTGTCACTCGTGGGTGAGCCGATCATGTACCCAGAAATCAACAGGCTTTTGAAGCTGCTCCACCAGCAC GGCATCTCCAGCTTCCTTGTCACCAATGCGCAGTTCCCTGAGGAGATCAGGAAGCTCACACCAGTTACTC AGCTCTACGTGAGCGTGGACGCCAGCACCAGAGACGGCCTGAAGAAGATCGACCGCCCGCTCTTCAAGGA TTTCTGGCAGCGCTTCCTAGACAGTTTGAAAGCCTTATCTGCAAAGCAACAACGAACGGTCTACAGACTG ACGCTTGTGAAGTGTTGGAACGTGGATGAACTCCAGGCCTACGCTGAGCTGGTGTCCTTGGGGAACCCCG ACTTCATCGAGGTCAAGGGTGTGACCTACTGTGGTGAGAGTGCAGCCAGCAGCCTCACCATGGCCAATGT CCCTTGGCACGAGGAAGTGGTTCGGTTTGTCCGTGAGCTGGTCGATCTGCTTCCCGACTATGAAGTTGCG TGTGAACACGAGCACTCCAACTGCCTCCTGATCGGACACAAGAAGTTTAAGATCGATGGAGAATGGTGGA CATGGATCAATTACAGCCGCTTCCAAGAGCTCGTTCTGCAGTACGAAGAGAGTGGAGGCTCCAAGACGTT CAGCTCGAGGGATTACATGGCCAGAACCCCACAGTGGGCACTGTTTGGTGCCAGGGAAAGAGGCTTTGAT CCCAAGGACACGAGATACCAGAGGAAGAACAAAACGAAGGACATTTCCGGATGTTGAGCTTATCTGAGTT AAGGATCTCCAAAGACAAAAGCTGCGTGGCCCCGGCAGGTCCTTGGCTGCCATCACTGTCCACGGGGCAA CGGTTCACAGCCGTACTTCATGGTCTGACACCAGCAGGCAGCCATGCTGCCCACACAGCATCCACAGGTC GACGCTGCTTACCAGAACTCATGCCCTTTAAAGAGTCAGTTTCTGAGACAGTGAAGGTGACAGTCACCTG AATTTTTACAGTTTGGGTTTTATTTTAGTAATTTTCCTAAGTGCTATCCCTGAGTGTTACACAGGGAATA AACACTGCCGCCTCCGCCTCCTGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6829456 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6829456 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11650 same embryo
	EMAGE:11649 same embryo
	EMAGE:11648 same embryo
	EMAGE:11652 same embryo
	EMAGE:11647 same embryo
	EurExpress:euxassay_008414 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source