EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11776

Slc28a1 solute carrier family 28 (sodium-coupled nucleoside transporter), member 1 ( MGI:3605073)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006962. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006962_01	euxassay_006962_02	euxassay_006962_03	euxassay_006962_04

euxassay_006962_05	euxassay_006962_06	euxassay_006962_07	euxassay_006962_08	euxassay_006962_09

euxassay_006962_10	euxassay_006962_11	euxassay_006962_12	euxassay_006962_13	euxassay_006962_14

euxassay_006962_15	euxassay_006962_16	euxassay_006962_17	euxassay_006962_18	euxassay_006962_19

euxassay_006962_20	euxassay_006962_21	euxassay_006962_22	euxassay_006962_23	euxassay_006962_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11776_wholemount_strong.wlz

11776_wholemount_moderate.wlz

11776_wholemount_weak.wlz

11776_wholemount_possible.wlz

11776_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11776_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9918
Entity Detected:	Slc28a1, solute carrier family 28 (sodium-coupled nucleoside transporter), member 1 ( MGI:3605073)
Sequence:	sense strand is shown >T9918 CTCCCACAGCAACCTGTGCTTCAGTCAGTCAGTTCTTAACAGTCAATCCCTCCTGACGCTGCCTTCTCAC TACAAATCTCTGAAGAGCCTAGCACATGGCAGACGACACACCGAGGCAACGAGAGTCCATTTCCCTCACA CCTGTGGCCCATGGCCTGGAGAACATGGGGGCCGAGTTCCTGGAAATAATGGAGGAAGGCCAGCTCCCAC ACAGGCACTCAAGCCTGCCAGAGGGTGGAGGCAGCAGGAGCAAAGCAGTGTGGAAACCCTTCTCCCGATG GAGGAGTCTGCAGCCGACTGTGCAAGCGAGAAGCCTCTGCAGGGAGCACTGGCAGCTGTTTGAGTGGATC AGCAAAGGCCTGCTCTCTACTGCATACATCAGCTTCTTGATCGTCGCCTGCCTCCTGGACTTCCCGAGGG CGCTGGCGCTGTTCGTCATCACCTGTGTGGTTCTCGTCTTTCTGGCCTACAATCTGCTAAAGAGGCTTCT GGGGTCCAAGCTGGAGAAGTGTGTGAAGTTTCAGGGCCACTCTTGCCTGAGCCTCTGGCTGAAGAGAGGT CTAGCCCTTGCTGCTGGCCTGGGTGTGATCTTGTGGCTATCTCTGGACACCGCCCAGCGGCCGGAACAGC TGGTGTCCTTTGCAGGGATCTGTGTGTTCCTTGTCCTTCTCTTCGCTGGCTCAAAGCATCACCGTGCTGT GTCATGGAGGGCTGTCTCCTGGGGCCTTGGGCTGCAGTTTGTGCTTGGACTCTTCGTCATCAGAACAGAG CCAGGATTCGTTGCGTTCCAGTGGCTAGGTGACCAGATCCGGGTCTTCCTGAGTTACACCGAGGCCGGCT CCAGCTTCGTCTTCGGGGAGGCTCTGGTCAAGGATGTCTTTGCCTTTCAGGTTTTGCCCATCATCGTCTT TTTCAGCTGCGTCATGTCAGTTCTGTACTATCTGGGCCTCATGCAGTGGGTGATCCTGAAGATTGCCTGG TTGATGCAGGTCACCATGGGCACCTCAGCCACTGAAACCCTAAGTGTGGCAGGAAACATCTTTGTGAGCC AGACTGAAGCTCCACTGCTGATCCGGCCCTATCTGGCAGATATGACACTCTCTGAAGTTCACGTTGTCAT GACTGGAGGCTATGCTACTATTGCTGGCAGCCTCCTGGGCGCCTACATCTCCTTTGGGATTGACGCTTCC TCCTTAATTGCTGCCTCAGTCATGGCGGCCCCTTGTGCTTTGGCCCTCTCCAAGCTGGTCTACCCAGAGG TGGAGGAGTCCAAGTTCCGGAGTGAGGAAGGCGTGAAGCTGACGTATGGAGATGCCCAGAACCTCGTGGA AGCAGCCAGCGCTGGGGCTGCCATCTCAGTGAAGGTCGTTGCCAACATTGCTGCCAATCTGATCGCCTTC CTGGCTGTACTGGCCTTCATCAACGCTGCCCTCTCCTGGCTGGGGGACATGGTGGACATCCAGGGACTCA GCTTCCAGCTCATCTGCTCCTACGTCCTGCGGCCTGTGGCCTTCTTGATGGGCGTGGCCTGGGAGGACTG TCCAGTAGTGGCTGAGCTGCTGGGCATCAAGCTGTTTCTCAATGAGTTTGTGGCCTATCAAGAGCTTTCC CAGTACAAGCAGCGACGCCTGGCAGGGGCTGAGGAGTGGCTTGGTGACAAGAAACAGTGGATCTCTGTCA GAGCAGAGATCCTGACGACGTATGCCCTCTGTGGATTCGCCAACTTCAGCTCCATTGGCATCATGCTGGG GGGTCTGACCTCCATGGTCCCCCAGCGGAGGAGTGACTTCTCCCAGATTGTGCTACGGGCACTGATCACA GGGGCCTTCGTGTCCCTGGTAAACGCCTGTGTGGCAGGGATCCTCTATGTGCCCCGGGGGGTCGAGGTGG ACTGCATGTCTCTTCTGAACCAAACTGTCAGCAGCAGCAGCTTTGAGGTTTACCTGTGCTGTCGCCAAGT CTTCCAAAACACCAGCTTGGAGTTTGGCCAAGAGGCACTGCACAACTGCTGTCGATTTTACAATCACACA GTCTGCACGTAGCTGGGACCAAATATCTTCCTAACCTCAAGGCTCATCCATCCTGGATAAGGCCATGGGA CTCACCACGACTTCCATCCCACAGCTGGGAAAAGGCGCTGCAGTGAATGACAGACGCCTCACACCCTGGT CCCGTTGCTGCTCCCCATGTCCTACCTCTCCAAGTGTGGGTTCCCAGTGTCTAGTCGGCCCCCTCGCCCT CTGAGAGCAGACATTCTGGTCCTCTCTATTCCTCTTTCTTTGGGACCCTCATGTGCACCTGCCCAAAGCC TCCTCCCTGCTCCCTTCCAAGCACCGGACTTGCTGGGTGCCCCCAGAAGCTGTCTCTAGAGACCTTCTTC CGCCCAGTCTCCAAGAGTGCTTTCCACCCCAGTGTCCTGTGGGGCACCATCTCATTAGGTGTGATGTCAG AGTGGCCCAGGCTCCACTCTGACTCGGTAAAGTTTGATGCGAGCTTGGTGGGTTTGTTGATGTTCTAGAT GGGTCTCATGTAGCTCCTTCTAGCATTGACCTTACTCTGTAGCTGAGGTCACCCTATTCCTCTGTCCTCC CGCCGTCACCCCTGAGTGTCAGGAGTACAGGTGCGCACTATCACACTGCGGCCATAAGGACTTCTTGTTT GGGCTGTTGAGAGATCTCAGTGGGTAAAGGCATCTGCTGCTAACTTGATGAACTGACTCTGATGCCTGGA CCCCCAAGGTGAAAGAGGGAAGCCATCTCTGCATGCTGTCCTCTGACCTCTACATATGTGCGTGGCACAT GCTCTGCCCCTCCCAACTAAAAATAAAGTATAACTTAAAACCTTTAAGCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30379959 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30379959 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11779 same embryo
	EMAGE:11777 same embryo
	EMAGE:11780 same embryo
	EMAGE:11778 same embryo
	EMAGE:11775 same embryo
	EurExpress:euxassay_006962 same experiment
	MGI:4828173 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source