EMAGE mouse gene expression spatial database

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EMAGE:11779

Rasgrp3 RAS, guanyl releasing protein 3 ( MGI:3028579)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006926. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006926_01	euxassay_006926_02	euxassay_006926_03	euxassay_006926_04

euxassay_006926_05	euxassay_006926_06	euxassay_006926_07	euxassay_006926_08	euxassay_006926_09

euxassay_006926_10	euxassay_006926_11	euxassay_006926_12	euxassay_006926_13	euxassay_006926_14

euxassay_006926_15	euxassay_006926_16	euxassay_006926_17	euxassay_006926_18	euxassay_006926_19

euxassay_006926_20	euxassay_006926_21	euxassay_006926_22	euxassay_006926_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11779_wholemount_strong.wlz

11779_wholemount_moderate.wlz

11779_wholemount_weak.wlz

11779_wholemount_possible.wlz

11779_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11779_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9917
Entity Detected:	Rasgrp3, RAS, guanyl releasing protein 3 ( MGI:3028579)
Sequence:	sense strand is shown >T9917 GCAGCCAGACAGGCCAAGCGCTGGTGTGAGGAAGACAAAATTACCCCAGTTTTCCAGAGCCCTTATCATT TGAGTTTAACGAACCAGTTCTTCACTCTGCTTCCTTCATGCCCTTCCCAGGGATGACAACTGCCCTTGGC ATCTTTTCAGACCTGGAACTCTATCTGTATGAAAATAGATTAGCATTCTTGAAGTATAATTAAGAAGGAA CATTACCTGCCAAAGGGCGAGAGAACCACTGCCTCGTACAGATATGGAGATAGTCTGTCTGCTGCTGGAA ATGTCTCCAGATTTTTGACAACGGCTAAAGAACCATGGGGTCCAACGGGCTCGGGAAAGCGGCAACACTA GATGAACTTCTCAGCACATGCATTGAGATGTTTGATGACAATGGAGACCTGAACGACAGTTACTTGCCCA GAATCGTGTTACTGATGCACCGATGGTATTTATCCTCCACTGAACTGGCAGGAAAACTCCTCTGCATGTA TCGGAATGCCAGTGGAGAAAGCTGTGATGAGTTCCGACTGAAGATCTGCTATTTCATGAGGTACTGGATT CTGAAATTTCCTGCAGAGTTTAATTTGGATCTTGGTTTGATTCGCATGACTGAGGAGTTCCGGGAAGTAG CTAGTCAACTAGGACATGAAAAACACATCAGCCTCATGGACATATCTAGCGTTCCTTCCTATGACTGGAT GAGGAGAGTCACACAGAGGAAAAAAGTATCCAAAAGGGGAAAGGCCTGCCTCTTATTTGACCATCTGGAG CCCATTGAGTTGGCGGAGCACCTCACTTTTCTGGAGCATAAATCTTTTAGAAGGATCTCATTCACAGATT ATCAGAGCTATGTCATCCATGGCTGTCTGGAGAATAACCCGACATTGGAGAGATCTATTGCTTTATTTAA TGGAATCTCTAAGTGGGTCCAGTTGATGGTTCTTAGCAAACCAAGTGCGCAACAAAGGGCAGAAGTCATC ACAAAGTTCATCAATGTAGCCCAGAAACTCCTTCAACTAAAAAATTTTAACACCTTGATGGCAGTGGTTG GTGGCCTTAGTCACAGCTCCATTTCCCGACTCAAAGACACCCATTCTCACCTCTCTTCCGAAGTTACAAA GAACTGGAATGAAATGACAGAGTTGGTCTCCTCCAATGGCAATTACTGCAATTACCGCAAAGCCTTTGCT GACTGTGATGGCTTCAAAATCCCCATCCTTGGCGTGCACTTGAAAGACTTGATAGCAGTCCATGTCATTT TCCCAGACTGGATGGAGGAGAACAAAGTGAACGTTGTGAAAATGCACCAGCTTTCTGTTACCCTGAGTGA ACTGGTCTCCCTGCAGAATGCCTCTCACCACTTGGAACCTAACATGGATTTGATCAACCTACTCACTCTC TCTCTGGACCTCTACCACACAGAGGATGATATTTACAAACTGTCACTGGTGTTGGAGCCTAGAAATTCCA AATCGCAGCCAACCTCCCCAACGACACCTAATAAGCCTGTGGTACCTCTGGAGTGGGCTTCAGAAGTGGT ACCAAAGCCAGACCCCACAATCATCAACAAACACATAAGGAAGTTGGTTGAGTCTGTGTTTCGAAACTAC GATCATGACCACGATGGTTACATCTCCCAAGAGGACTTTGAGAGCATAGCTGCCAATTTCCCCTTCCTGG ATTCCTTCTGCGTACTAGACAAAGATCAGGACGGCCTAATTAGCAAAGACGAAATGATGGCTTATTTCCT GAGAGCTAAATCTCAGCTACACTGCAAAATGGGACCAGGCTTCATCCATAATTTTCAGGAGATGAACTAT CTCAAGCCGACCTTCTGCGAACACTGTGCGGGATTTCTCTGGGGCATAATCAAACAAGGCTACAAGTGCA AAGACTGTGGAGCCAACTGCCACAAGCAGTGCAAAGACCTCTTGGTTCTGGCCTGCAGGAGACTTGCCAG GGCACCCTCCTTGAGCAGCAACCCCGGGTCACTGCCTGGAAGCCCAGCCTTGCCCCCAGTGCAGGATGAG GTATTTGAGTTTCCTGGGGTCACGGCTGGACACCGAGACCTGGACAGCAGAGCCATTACCCTGGTTACAG GATCTTCCCGGAAGATCTCCGTGAGGCTGCAGAGAGCCACCACTAGTCAAGCCACCCAGACGGAACCTGT GTGGTCAGAGGCTGTCTGGGGAGACTCAGGGTCCCATACCTTCCCCAAAATGAAATCCAAGTTCCATGAC AAAGCAGCAAAGGACAAGGGCTTTGCCAAGTGGGAAAACGAGAAGCCCACGGTACAAGCTGGTGTGGATG TTGTAGATCGAGGCACTGCATTTGAACCTGACCAGGATGATGGGCAAGATGAAGCCAAGCAGGGTGGTGA GGATGGCTGAGGTCAAGCTTGGGAAACCAAAGGCAATCAAGTCGGCTATGAGGAGGAGAGAGAAGAGGCG CTCTGAAAGGCACGGTCTCTCTCAGAAGCGGTCTGGAAAAGAGATTTGGATATGCTCACAGCCTCCAGTC ATCCTGGAATCCCCATGACTGGACTACGGGATAGAGAATTTACCCTGACTGAACTATTTATTTCCTGTAC TCTGTCTGCCCCTGCTGAGGTGCCACGAAAACTACTTAAGCAGCTTCCCACATCTCTCTAAAGCCATTTG TAGCCAGGGGGATGAAAGCTTTGGTGCACGTGCGTGGTTTTCTCTAAAATCAAAACTTAGCTCTTTTGGA CAATATTATTTTTGTAAAGTGTGTGCGTGTGTGTAGGGGTGTCTCAAAAGAAGTTGAATTTGAGCTCCTT TGTGTGTGCCTTTTAGATGGGACCAGGACTTGAAGTTTTATTGAGGGACAAAGACATTCTTTAGACCCTG AAAGGCAGTGAACCCTCTGCTGCTGATTGGCTGTAAGGAGTGTTTATAGTGTCCTACCACACAGTGGCTG TGCTTGTAATGCCATGTGTGTGTATCACCTAGGTCTCAATCAATCCTGGATCACTAAGGGGAATCATATC CCCAAAGCCCCCCTAATACTCATGCTAGATATGGTAAAAACAAACAAAACAAAACAAAACAAATCAAAAC AGCATACACCTGGCCACCTAATTGAGTTCCCCACTCTTTCTGAAGATTCCCAAAATAGAATGTTAATTAA GAAAGCAAATCAAGTTCTGAGGGATGAAATTTAGTAGAAAGACCCACCAAATTACTAGGTTAAAGATAAA CAACCAGTTTGGTAAGCTGAAAGTAAGCCTATGATTTTTATTTTAGATAATTTCTAGATGCAAAAGGTAA CTTAATTTCTTCCTAATAAATTCTCACCCATAGTTAGAAAAACATTTACAATTCTCATCTCTAGCTCTTC TTCTTCCCCACCCTTCCCCCCTTTCTCCATGTGGCCATGGCTGGCCACAGGGCTCTCTCTTCCAACCTTC TCTCTTCTTCTCTGCCTTTCTACAGTAAAGCTTTAAAACCATGAAAAAAAAAATTCACAAAAGAAAAAAT GACCATAAACTTCAACTATGGATTGATATTCCATGTACACATTTAAAATATTAATAATTTAAATGTCTTT CTTTGAGTGTGTATCTTTTGAAATAGAGACGCCAGGCTGATATTCCAATACCTATAATCTCTGCACTTGG GCCATGAGATAGGAGGACCCCAAGTCTGAGGTCATCCTGGGCAATTTAGCAAGAATCTGTCTAAAAAAAA AATGTAGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGGCTGCTCTTCTAGAGGTCCTGAGTTCAA TTCCTAGCAACCACATGGCGGCTCACAACCATCTAGGGATCTGATGCCCTCTCCTAGTATGTCTGAAGAC AGCTACAGTGCACTCATATACATAAGTCTTTAAAAAGTAATAATCAAAATAAAAGAAAAATTGAGGTGAT TGAGTCTGTCATTGAGGGAACCATATCATATCAAATAACCAGTTAGGAAATATTCCAGGTGGGTACCAGG TGGGCTAACGTGTGTATCCCATGTAAAACTGACTATGGTCTTATTTGTGAAAGTCACTTCCATACAGCTC AACTGCTAATACTATGCTCCTCCTTAGAAATGGAGAGCATGAGGCCATAACTGCTAAGTGGTTCCAGTGT CTTAAGACAACCTTGGTAGAGGTGACACAATGTGCCCCGAAGGTTCTGGAATGGGGCATGATTCAGATTG GGGATACTAAACAATAAGTGATGTCTCTACTTGAAGGAGCCGTGATTAACTAGATGAGAACGAGAACTCA TTAATGGTCGAGCGCCTCGGGCAGTGCTGTTGGATCTATGTATATAGACTGTTACTATTCATAAATACAG CAGAGTACGTGTTAACGGATTCAAAACAGAAACTGACAAGTCAAGTGCTGGCAAACCCTCTAAAAACACC CAGAAGGAAACCTTAGGGAAGGCTGGAGTCTTTGTATTTCTTTTCAAAGGAATTATCTCACCTGCCATTT GGTTTATATATTTATTAGTTTTCATATATAGAAGGAATTTTGTATCTCTGGTCTTCCTATGATAAGGAAG ACAAAATGTACCTTCAATGAAAAATAAACATACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30280332 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30280332 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11777 same embryo
	EMAGE:11780 same embryo
	EMAGE:11778 same embryo
	EMAGE:11775 same embryo
	EMAGE:11776 same embryo
	EurExpress:euxassay_006926 same experiment
	MGI:4827630 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source