EMAGE mouse gene expression spatial database

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EMAGE:12107

Atg4a autophagy-related 4A (yeast) ( MGI:2147903)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008404. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008404_01	euxassay_008404_02	euxassay_008404_03	euxassay_008404_04

euxassay_008404_05	euxassay_008404_06	euxassay_008404_07	euxassay_008404_08	euxassay_008404_09

euxassay_008404_10	euxassay_008404_11	euxassay_008404_12	euxassay_008404_13	euxassay_008404_14

euxassay_008404_15	euxassay_008404_16	euxassay_008404_17	euxassay_008404_18	euxassay_008404_19

euxassay_008404_20	euxassay_008404_21	euxassay_008404_22	euxassay_008404_23	euxassay_008404_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12107_wholemount_strong.wlz

12107_wholemount_moderate.wlz

12107_wholemount_weak.wlz

12107_wholemount_possible.wlz

12107_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12107_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9989
Entity Detected:	Atg4a, autophagy-related 4A (yeast) ( MGI:2147903)
Sequence:	sense strand is shown >T9989 GGTAGTCAAGTTGCCGGTGGAATTGGCCCAGGATGACAGCTGGAGAATGGAGTCAGTTATGTCCAAGTAT GAAAACCAGATTCTTATTTTCCCTGACTACCTGGAAGAATTCCCGGATACAGATGAGCTGGTATGGATTC TGGGGAAGCAGCACCCCCTTAAGACAGAAAAATCTAAGCTGTTGTCTGATATAAGTGCTCGTCTATGGTT TACATACAGAAGGAAGTTTTCCCCGATTGGGGGAACAGGCCCTTCATCTGATGCTGGATGGGGATGTATG CTGCGCTGTGGGCAGATGATGCTGGCTCAAGCCCTCATCTGCAGACACTTGGGAAGGGATTGGAACTGGG AGAGACAAAAAGAACAACCCAAAGAATACCAAAGGATACTGCAGTGTTTCCTAGATAGAAAAGACTGTTG CTATTCAATCCATCAGATGGCACAAATGGGTGTAGGAGAAGGGAAATCAATTGGCGAATGGTTTGGACCA AACACAGTTGCACAGGTGATAAAAAAACTCGCTTTATTTGATGAATGGAATTCCTTGGCTGTTTATGTTT CCATGGATAACACAGTGGTCATTGAAGATATCAAGAAAATGTGCTGTGTTCTTCCTGTGGGTGCTGCTGA CCCAGCTGGTGATTTTCTGACTGCATCCAATCAGAGTAGAGACACCTCTGTCCCGTGCTCAGCCTGGAAA CCCCTGCTGCTCATTGTGCCCCTTCGCCTGGGCATAAACCAAATCAATCCTGTGTATGTTGAAGCATTCA AAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGCTTTAGGAGGAAAGCCAAATAACGCCTATTATTTCAT AGGATTCTTAGGAGATGAGCTCATTTTTTTGGACCCTCACACAACCCAGACTTTTGTTGACATTGAAGAG AGTGGACTAGTAGACGACCAGACTTTTCATTGCCTGCAGTCTCCACAGCGGATGAGTATCCTGAACTTGG ATCCTTCTGTGGCCTTGGGATTTTTCTGCAAAGAAGAGAAAGACTTTGATAATTGGTGTAGCCTTGTTCA GAAGGAAATTCTAAAGGAGAATTTGAGGATGTTTGAATTGGTTCAGAAGCACCCATCACACTGGCCTCCC TTTGTACCTCCAGCCAAGCCAGAAGTGACAACCACAGGGGCAGAATTCATAGAGTCGACTGAACAACTGG AGGACTTTGAGCTGGAGGAAGATTTTGAAATTCTGAGTGTAGGCTAGTTAGAACTCAGTGAGGTGGTCTT CTGCCTTCCATCGGGCATCACAAGCACATGAAAACTTTTCTGGTCAGCAAGTGCCTGATATGCAGATAGC ATACAAACTCAATAACAATGATGACTGAGCCAGTTGTCCCTTGTTCAGAAGCAAAACAAAAACAGCAAAT CAATGGCACCGAGTCACCCTGGAAAGACAGAAAACTATGGAGTCTAGGAGAAGATTGAGAATCCATTGCT CCCCAGCTCGTCTTACATGGCGACAGCAAGTCTTCAGCAATGGAAACGAGGGCATTTGGAAAGCAGACAG CATCTTCCATCTTGCTGCAACTATGGGCAGATGAGAGATGGTTATACTATACTACTCCACCCTTTCAGGT GGGGCTAAATACTAAGAATAAATCTGTTTTCTTGTTCAAATAATAAAATAAATGAATCAGGGAGAAATAC TCTTGTTAAATTATCTGAATATGCAGTTTACATAATTGTAATTTTTATCAAAATATTTGTCAAAATGATG TCAAATATACACTTCTTAGTTGTCTTCCTTTTGGAGAAACCTGTTTGATAGGTCACAATCTCCATCCTTA CTGATGCTTTCATTAGATGTCACCGTATCCTTCAATCCAACCATGATCACTTGAAGCAGAGATCAGCAAA CTTTTTCTGTAAAGATAATATTTTAGTCTTTGTAGGCTGGGTGGCCTTTGTTGTAACTATTCTCCTCTGT AAAAGCAGTCCCAGACAATATGCATGTGACCGAGCATGGCTGTACTCTAATAAAATTTGATGTACAAAAG CAAGAGGAAGGCTGGACTGGCCTTACCAATCGCTCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30286094 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30286094 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12106 same embryo
	EMAGE:12111 same embryo
	EMAGE:12108 same embryo
	EMAGE:12109 same embryo
	EMAGE:12110 same embryo
	EurExpress:euxassay_008404 same experiment
	MGI:4823295 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source