EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12110

Acap3 ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 ( MGI:2153589)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008406_01	euxassay_008406_02	euxassay_008406_03	euxassay_008406_04	euxassay_008406_05

euxassay_008406_06	euxassay_008406_07	euxassay_008406_08	euxassay_008406_09	euxassay_008406_10

euxassay_008406_11	euxassay_008406_12	euxassay_008406_13	euxassay_008406_14	euxassay_008406_15

euxassay_008406_16	euxassay_008406_17	euxassay_008406_18	euxassay_008406_19	euxassay_008406_20

euxassay_008406_21	euxassay_008406_22	euxassay_008406_23	euxassay_008406_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12110_wholemount_strong.wlz

12110_wholemount_moderate.wlz

12110_wholemount_weak.wlz

12110_wholemount_possible.wlz

12110_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12110_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9173
Entity Detected:	Acap3, ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 ( MGI:2153589)
Sequence:	sense strand is shown >T9173 GTCCTCGGCCTCACAGGGGCGCGATGATGGGTCGCTTCCGCACAAGCGACCTTAATTCGTTCACTCCCGA TCTCCCCGCCGCCGTCGCGGAGTGTGTCCGTCGGCAGCATTCCAGCCGAGTTCACCACCGCCGTGATGCC TGAAGTGGGCGAGCCGTGCCCTTCCCTGTCCCGCGACCGAGCCAAACCACTCCGAAGCCCAACCGCCGAG GCCGGCCCAGCTCCCAGACCGTGGTGACCGAGGACAGAGCGCAGCGACAGGCGAGGGGATGCAGCGGCGC TTTTAGCGACGCTCCCCGGAGGTGACAGCGGGTGGCGCAGGCAGAGGCTGACTTTGGCGACGCTCCCCAG CGGTGACAGGCGGGGCGGGGCCGCGGCGCGCCGGGCTCAGACCGAGGCGGGCAGGCGGGCGGGCGGGCGC GGAGCGCAGCTCGGCGCGGGCGCCACAGACGGTCCACGGGCCGGGGATGCCGCTCGACGCGCCTTGGTCC TCCGCACAGTGAGCACCGCTCGCCGCAGCCATGACGGTAGAGTTCGAGGAATGCATCAAGGACTCGCCGC GCTTCAGGGCAACTATTGATGAGGTAGAAACAGATGTGGTTGAGATCGAGGCTAAACTGGACAAGCTGGT CAAACTGTGCAGTGGTATGATTGAAGCCGGCAAAGCCTACGTCACCACCAACAGGCTCTTTGTGAGCGGT GTCCGAGACCTGTCTCAGCAGTGCCAGGGCGACACCGTCATCTCGGAATGTCTGCAGAGGTTTGGAGACA GCCTACAGGAGATGGTCAACTATCACACGATCCTGTTTGACCAGGCCCAGAGATCGGTGCGGCAGCAGCT CCACAACTTTGTCAAAGAGGATGTGCGGAAGTTCAAAGAGACAAAGAAGCAGTTTGACAAAGTGCGGGAG GACATGGAGTTGTCCTTGGTGAGGAATGCCCAAGCCCCAAGGCACCGGCCCCATGAAGTAGAGGAGGCCA CAGGTGCCCTCACTGTCACCCGGAAGTGCTTCCGCCACTTGGCGCTGGACTATGTGCTGCAGATCAATGT CCTCCAGGCCAAGAAGAAGTTTGAAATCTTAGATTCTATGCTGTCCTTCATGCATGCCCAGTACAGCTTC TTCCAGCAGGGGTATAGCCTCCTACATCAGCTGGACCCCTACATGAAGAAGCTGGCAGCTGAGTTGGACC AGCTTGTGATCGACTCTGCAGTGGAAAAGCGAGAGATGGAGCGCAAGCATGCTGCCATCCAGCAACGGAC ACTCCTGCAGGACTTTTCCTATGATGAACCCAAAGTGGAGTTTGATGTGGATGCACCAAGTGGTGTGGTA ATGGAGGGCTATCTCTTCAAGAGAGCCAGCAATGCCTTCAAGACATGGAACCGACGGTGGTTCTCCATTC AAAACAGCCAGCTGGTCTACCAGAAGAAACTCAAGGATGCACTCACTGTGGTGGTAGATGACCTTCGCCT ATGCTCTGTGAAACCATGTGAGGACATTGAACGGAGGTTCTGCTTTGAAGTCGTGTCACCTACCAAGAGC TGTATGCTGCAGGCTGACTCGGAGAAGCTGCGACAGGCTTGGGTTCAAGCTGTGCAGGCTAGCATCGCCT CTGCCTACCGGGAAAGTCCAGATAGCTGCTACAGTGAGAGGCTGGACCGTACAGCGTCGCCATCAACAAG TAGCATTGATTCCACCACGGACTCTCGGGAGCGTGGAGTCAAGGGCGAGAGCGTGCTGCAGCGTGTGCAG AGTGTGGCTGGCAACAGCCAGTGTGGCGACTGTGGCCAGCCAGATCCCCGCTGGGCCAGCATCAACTTGG GTGTGCTGCTCTGTATTGAGTGCTCAGGCATCCACAGGAGCTTGGGCGTCCACTGCTCTAAGGTACGATC TCTGACACTGGACTCCTGGGAGCCAGAGCTGCTAAAGCTGATGTGTGAGCTTGGAAACAGCACCGTGAAC CAGATCTATGAAGCCCAGTGTGAGGGCCCAGGCGTTAGGAAACCCACAGCCAGCAGTTCCAGGCAGGACA AAGAGGCATGGATCAAGGACAAATACGTTGAAAAGAAGTTTCTACGGAAGTTGACTTCTGCACCAGCCCG GGAACCCCCAAGACGCTGGAGGGCACAGAAGTGCCAGCGCCCTCACAGCTCCCCCCACGCCCCCACTACC CGCCGCAAGGTCCGGCTTGAGCCCGTCCTGCCCTCCGTTGCTGCTTTGTCCTCAGCTGCTACTATGGAGC GCAAGTTCCGCCGAGACTCCCTCTTCTGCCCTGATGAGCTGGACTCCCTCTTCTCTTACTTTGATGCAGG GGCTGCTGGGGCTGGTCCACGCAGTCTAAGCAGTGACAGTGGTCTAGGAGGCAGCTCTGATGGCAGTTCA GATGTTCTTGCCTTCGGCACAGGCTCAGTGGTGGACAGTGTCACCGAGGAAGAGGGTGCCGAGTCCGAGG AGTCCAGCAGTGAGGTCGATGGAGAAGCAGAGGCCTGGAGCTTGGCAGATGTGCGTGAGCTGCATCCTGG GCTGCTGGCACATCAGGCAGCTCGTACCCGTGACCTCCCAGCACTGGCTGCAGCGCTGGCCCATGGAGCT GAAGTCAACTGGGCCGATGCAGCAGATGAGGGCAAGACCCCATTGGTGCAGGCTGTACTAGGGGGTTCCT TGATTGTCTGTGAGTTCCTTCTGCAAAACGGAGCTGATGTGAACCAAAGAGACAGCCTTGGCCGGGCCCC CTTGCACCATGCTACACTCTTGGGCCGTACCGGCCAGGTCTGTCTGTTCCTGAAGCGGGGGGCTGACCAG CATGCCCTGGACCAGGAGCAACAGGACCCACTGACCATCGCAGTTCAAGCAGCGAATGCTGACATTGTCA CATTGCTTCGCCTGGCCCGCATGGCTGAGGAGATGAGAGAAGCTGAGGCGCCCCCTGGTCAGCCGGGACC CCTGCCGGGCAGCAGTCCTACAGAGCTGCAGTACCGCAGGTGCATCCAGGAGTTCATTGGCCTCCACCTG GAGGAAAGCTAGGACCTGAAAGGCCGGGCAGCTGCGGGAGCCCTACCCTGACCCCAGCCTACCCGGCCCA GAAATGTTGCATGTCCCTGAAGACCCTAGCCCCTCATCTAGCCACAGCCCTGCCTGTGACAACTGTGGGC TCATCCCAACAGCCCAGGACCCTGGTCTTCCTGGAGTCCTCACTCCTGCTTTTCCCTCCTTCCGGCTGCC CTGATGCTCAGCCAGAGGACAGGAACCCGAGCACTACATCATTTGAAGCCTCACACTTGTGGGTGCTGCA TCTCGTCTGGTGCCCCTCATTTCACCCGGGGGAACCTGTTTTCAGGTGGTCCTTCCTTCGGGGGCCTGGG CTGTCCATGATGTCACCCTGTGCCCCGCTGCCCTCAGGGCCCGTTTCAACTTGTGCCCCTATGTTTACTG TCCCCAAACCCTGGCCTGCTAGCTAGGTCCTTCCTCCAGCCTAGGGCTGGTGGCTGGCCACCAGCACCTT CCCTGGCAGGAGTGGCAGCCCACCCAGGGAGTCACCCTGAGTACCTTGCTCGTGACCCCAGCATCTGGGT TGCGCTTTGATGGTGCTGGGAGGTGGAGCTTTGGCCCAGGTCTTCCACCCAGCCTGGACGCAGGCGTCCC AAAGCCACGTGGCTGGCCACTTGGGTCCTTGTGCCCGAGGGCTCAAGTCCACCCCTGCCTCCTCCAGAGG CACTGTCTGGTTTACCTGGGCCAGCACCCATGCCTAAAGCAGCTGGTACTCTCCACCCTGTGCCCAATGT TCTTTGTTACTGAGAGGATTTTCTACAACAACCTCATTCTGACTTTGTCTGCGTCTTTTTTCTGTGGACG CTCAAGGACCATCCTGATCCCGGCTCTGCCTGCTGCTGGGGAGGCCCACATAGATTTCCCCCCTGCCCTG CCCCCGAGCTTCACTCCTTGGGCTGGGCCTCTGATATAGGTCCAGTGGTCCCTTATGTGGGGGGCCCACC AATGCCTTCAATTTCTTTCTTTCTGGAGGAGTCCCAGGACAGAGGCTGGGTCCCTCTGCATACTATGGGG CCCTTGGCTAGGTCAAGGGCACTCAGCTGGTCACTATGCAAACACTGCCCTGGGAGCATCCATGGACAGT AATGTCGGCACTGGGTCAGGGCCTCTGACTCCCTTGCTTGATGGCTGTGGCCCTACAGAGCAGAATCAGC TTCTCCGTCCTGCTTGAGCTCTGCCCACCACCTACCCTGCCACCTTTGGGCTGGCAGGGACCCACTCCTT CCTGCTCTGATCACACTCACTTAATAAATCTCTGCCGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827624 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827624 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12106 same embryo
	EMAGE:12111 same embryo
	EMAGE:12108 same embryo
	EMAGE:12109 same embryo
	EMAGE:12107 same embryo
	EurExpress:euxassay_008406 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source