EMAGE mouse gene expression spatial database

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EMAGE:12108

Gpr64 G protein-coupled receptor 64 ( MGI:2446854)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008379. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008379_01	euxassay_008379_02	euxassay_008379_03	euxassay_008379_04

euxassay_008379_05	euxassay_008379_06	euxassay_008379_07	euxassay_008379_08	euxassay_008379_09

euxassay_008379_10	euxassay_008379_11	euxassay_008379_12	euxassay_008379_13	euxassay_008379_14

euxassay_008379_15	euxassay_008379_16	euxassay_008379_17	euxassay_008379_18	euxassay_008379_19

euxassay_008379_20	euxassay_008379_21	euxassay_008379_22	euxassay_008379_23	euxassay_008379_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12108_wholemount_strong.wlz

12108_wholemount_moderate.wlz

12108_wholemount_weak.wlz

12108_wholemount_possible.wlz

12108_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12108_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 04 weak expression: see section 01 03 05 07 08

foot

moderate

regional

moderate expression: see section 04 weak expression: see section 01 03 05 07 08 22 23 24

facial vii ganglion

strong

single cell

strong expression: see section 05 23 moderate expression: see section 04

trigeminal v ganglion

strong

single cell

strong expression: see section 05 06 07 08 09 10 21 22 23 moderate expression: see section 03 04 19 20

dorsal root ganglion

strong

single cell

strong expression: see section 07 08 09 10 11 12 13 14 15 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35217
Entity Detected:	Gpr64, G protein-coupled receptor 64 ( MGI:2446854)
Sequence:	sense strand is shown >T35217 CATAAGCTAAATGAGGGCAACCAGTTACTATCAGAAGAAACCACGACTGGTTATGGTTTCTAGACGTTTG CAGTTTGGTTTCTTATCTCTCATTTTCAAAGGCATTGGTGTTAAAACCACAGAATAAGATCCAGGAAAGA AAAATGAAAGTTTGTTTCCACTCACATTTTAAAGAGGCTGTTATCCTTGATAACATCCTAAGAAAGAAGG AAAAAAAGAAGGAAAGAAAGAAAGAAAGAGAGAGAGAGAGAGAGAAAGAAAAAAGAAAGAAAGATGTTTA CTTCAGTCTGTTGGTAAGTTTAGTTGTGCATGCCTTTGTTGTATATAAGCTAAATTCTAGTGACCCACAT GTCAAAAAATCTTCTCTTTACCTTTTTTTATATTTATTTTCTACTGTGTAAATTTATTCTTTTGTAGAAT CATGGTTGTGTCGCCTTCTCTAATGTGATAACTTACCAGTGACTTCTAAACCTCCTTTAGAAGATCATAT AGGATGTGAAATTCAGAAACTTCACTGAAATCAAGAAATAATGATAAAGGCAATCTGAACAATTATAAAT AGACAATTAGCTCAGAAAGTGCCTTTGAGCAATCAAGAAAAGGTGCCCCCACTGGGCAGATATAGCCTTG TGAGCTTATGGTTTGGAAAACAGTCGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94099. Forward Primer - name:094099_F_cDNA_Gpr64, sequence:CATAAGCTAAATGAGGGCAACC; Reverse Primer - name:094099_N_SP6_cDNA_Gpr64, sequence:CCGACTGTTTTCCAAACCATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12106 same embryo
	EMAGE:12111 same embryo
	EMAGE:12109 same embryo
	EMAGE:12107 same embryo
	EMAGE:12110 same embryo
	EurExpress:euxassay_008379 same experiment
	MGI:4825192 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source