EMAGE mouse gene expression spatial database

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EMAGE:12663

Akap2 A kinase (PRKA) anchor protein 2 ( MGI:1306795)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011439_01	euxassay_011439_02	euxassay_011439_03	euxassay_011439_04

euxassay_011439_05	euxassay_011439_06	euxassay_011439_07	euxassay_011439_08	euxassay_011439_09

euxassay_011439_10	euxassay_011439_11	euxassay_011439_12	euxassay_011439_13	euxassay_011439_14

euxassay_011439_15	euxassay_011439_16	euxassay_011439_17	euxassay_011439_18	euxassay_011439_19

euxassay_011439_20	euxassay_011439_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12663_wholemount_strong.wlz

12663_wholemount_moderate.wlz

12663_wholemount_weak.wlz

12663_wholemount_possible.wlz

12663_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12663_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 08 09 10 16 17 18

thymus primordium

moderate

regional

moderate expression: see section 10 11 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 14 15 weak expression: see section 13

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 13 14 15

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

bladder

moderate

regional

moderate expression: see section 11 12 13 14 15

urethra of male

moderate

regional

moderate expression: see section 13 14 15

trachea

moderate

regional

moderate expression: see section 12 13

clavicle

moderate

regional

moderate expression: see section 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30188
Entity Detected:	Akap2, A kinase (PRKA) anchor protein 2 ( MGI:1306795)
Sequence:	sense strand is shown >T30188 ACACAGGCAGCCAAGGAGCAGAAAGCTCCCTGTGTGTCTGAAAGCCAGTCTGCAGGAGCCGGTCCAGCTA ACGCTGCCACTCAGGGAAAGGAAGGGCCATACAGTGAGCCTTCGAAACGCGGGCCCTTATCTAAACTGTG GGCAGAGGATGGAGAGTTCACAAGTGCCCGGGCCGTCCTGACTGTAGTCAAAGATGAAGATCATGGGATT TTAGACCAATTTTCAAGATCGGTCAATGTATCTTTGACTCAGGAGGAGCTGGACTCAGGGTTAGATGAGT TGTCAGTGAGGTCTCAGGATACCACTGTCCTGGAGACTCTGTCCAATGACTTCAGCATGGACAACATAAG TGACAGTGGAGCTTCTAACGAGACACCCAGTGCCCTTCAGGAAAACTCGCTGGCTGATTTCTCTCTGCCT CAGACCCCCCAAACAGACAACCCTTCCGAGGGCCGAGAAGGGGTCTCCAAGTCATTCAGTGACCATGGTT TCTATTCTCCTTCCTCCACATTGGGAGATTCTCCGTCCGTTGATGACCCCTTGGAATATCAGGCTGGGCT CCTGGTGCAGAATGCCATTCAACAAGCCATAGCCGAGCAGGTGGATAAGGCTGAGGCGCACACCAGCAAG GAAAGATCAGAACAGCAGGAGCCTGAAGCTGGAAGCCAGACACCTGGCTCTGAGAAGCCCCAGGGCATGT TTGCACCACCTCAGGTGTCCTCTCCTGTTCAAGAGAAAAGGGATATATTACCAAAGAACCTCCCTGCGGA GGACAGGGCACTCAGGGAAAAGGGACCCTCCCAGCCACCCACGGCTGCGCAGCCCAGCGGTCCAGTCAAC ATGGAAGAGACCAGGCCTGAAGGAGGGTACTTCAGCAAGTATTCAGAGGCAGCTGAGCTGAGAAGCACAG CCTCCCTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3493391), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10465. Forward Primer - name:010465_F_IRAV04-07_K22_Akap2, sequence:ACACAGGCAGCCAAGGAG; Reverse Primer - name:010465_R_SP6_IRAV04-07_K22_Akap2, sequence:AGGAGGGAGGCTGTGCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12668 same embryo
	EMAGE:12666 same embryo
	EMAGE:12665 same embryo
	EMAGE:12667 same embryo
	EMAGE:12664 same embryo
	EurExpress:euxassay_011439 same experiment
	MGI:4823061 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source