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EMAGE:13020

Ascl2 achaete-scute complex homolog 2 (Drosophila) ( MGI:96920)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020
"Pseudo-wholemount" of euxassay_013683. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013683_01 euxassay_013683_02 euxassay_013683_03 euxassay_013683_04
EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020
euxassay_013683_05 euxassay_013683_06 euxassay_013683_07 euxassay_013683_08 euxassay_013683_09
EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020
euxassay_013683_10 euxassay_013683_11 euxassay_013683_12 euxassay_013683_13 euxassay_013683_14
EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020
euxassay_013683_15 euxassay_013683_16 euxassay_013683_17 euxassay_013683_18 euxassay_013683_19
EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020 EMAGE:13020
euxassay_013683_20 euxassay_013683_21 euxassay_013683_22 euxassay_013683_23 euxassay_013683_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13020Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13020_wholemount_strong.wlz
13020_wholemount_moderate.wlz
13020_wholemount_weak.wlz
13020_wholemount_possible.wlz
13020_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13020_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31998
Entity Detected:Ascl2, achaete-scute complex homolog 2 (Drosophila) ( MGI:96920)
Sequence:sense strand is shown

>T31998
TAACACCCGCTACTCCGCCGTCCGATGAGTGCGCGCAGCCCTCTGCCTCCCCTGCCAGCGCGTCTCTGTC
CTGCGCCTCTACGTCTCCGTCCCCGGACCGCCTGGGCTGCTCTGAGCCTACCTCCCCGCGCTCCGCCTAC
TCGTCGGAGGAAAGCAGCTGCGAGGGAGAGCTAAGCCCGATGGAGCAGGAGCTGCTTGACTTTTCCAGTT
GGTTAGGGGGCTACTGAGCATCCCACCCCCCTAAGCTGCATCCCTGGGTGACTCCTGGTGGACCTACCTG
CTTCTAGCCCAAGAAACCTGGGCCTATGCCTTACCCATGCTGTCTAGTGCAGCCTGACCAAATGCCAAGT
GCTGACTGACCTCTGCTCGGCCTCCACGCCGCGGAATGACATCTTCCATCTTCCGGACCTTGCAGCATCA
GGACTTGGAAATTTCTCAGGATAAAGATTTTTACAATGACAATCTACTTTTTATCAATTAACTTGAACTG
TTGTAGGACTCTACTGAAAATATGAAGAATTATTTTTATACAAAGGATCCTTAAGCTTGGAGCACAATAA
AGATGACCTCTGTCCCTCACCCCCACTGTCTAGAACTTTCCAACCTGGCCAAAGTGTGGACCGGTTGGGC
CCTGAGGGCAAGATGCCTGGCTGCACCCTTCTTCCTCTTCTGAAGCCTATACTGACGCTGATGTTTGGCC
AGTGTGGGAACCCTGCTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4163833), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22686. Forward Primer - name:022686_F_IRAV32-35_D01_Ascl2, sequence:TAACACCCGCTACTCCGC; Reverse Primer - name:022686_R_SP6_IRAV32-35_D01_Ascl2, sequence:AACAGCAGGGTTCCCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13019 same embryo
 EMAGE:13023 same embryo
 EMAGE:13022 same embryo
 EMAGE:13018 same embryo
 EMAGE:13021 same embryo
 EurExpress:euxassay_013683 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS