EMAGE mouse gene expression spatial database

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EMAGE:14164

Ugt2a3 UDP glucuronosyltransferase 2 family, polypeptide A3 ( MGI:1919344)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001860. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001860_01	euxassay_001860_02	euxassay_001860_03	euxassay_001860_04

euxassay_001860_05	euxassay_001860_06	euxassay_001860_07	euxassay_001860_08	euxassay_001860_09

euxassay_001860_10	euxassay_001860_11	euxassay_001860_12	euxassay_001860_13	euxassay_001860_14

euxassay_001860_15	euxassay_001860_16	euxassay_001860_17	euxassay_001860_18	euxassay_001860_19

euxassay_001860_20	euxassay_001860_21	euxassay_001860_22	euxassay_001860_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14164_wholemount_strong.wlz

14164_wholemount_moderate.wlz

14164_wholemount_weak.wlz

14164_wholemount_possible.wlz

14164_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14164_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4952
Entity Detected:	Ugt2a3, UDP glucuronosyltransferase 2 family, polypeptide A3 ( MGI:1919344)
Sequence:	sense strand is shown >T4952 GGCACAATATGGTCTCTGAAAAATGTGTTGCGGCATTTTTTCTGCTGCAGCTTTGCTGGGCCGGCTGTGG ATTCTGCAGCAAGGTCCTCGTGTGGCCCTGTGATATGAGCCACTGGCTGAATCTAAAGACTATTCTTGAG GAGCTTGGAGCAAGAGGGCACGAGGTAACAGTCCTGAAATACCCCAGTATCATCATAGATCAGAGTAAAC GTATTCCACTGCACTTTGAGAATATTCCTTTGCTGTATGAAATCGAGACAGCTGAGAATCGTTTAAATGA GATTGCAAATCTAGCTGTGAATGTCATTCCAAACCTGTCACTGTGGGAAGCAGCAAAAACATTACAAGAC TTCTTTCTTCAAGTAACTGGAGATTTTGAAAGTATTTGTAGGAGTGTATTGTACAACCAGAAATTCATGG ACAAGCTACGGGATGCACAATATGATGTAGTGGTTATAGACCCTGTCGTTCCCTGTGGAGAGTTGGTGGC AGAAGTGCTTCAGATCCCTTTCGTATACACACTGAGGTTCAGCATGGGCTACTACATGGAGAAACACTGT GGCCAGCTTCCAATTCCACTCTCGTATGTACCGGTTGTCATGAGTGAGCTGACAGACAATATGACCTTCA CAGAGAGGGTGAAAAATATGATGTTTTCACTGTTGTTTGAGTACTGGCTCCAGCAATATGACTTTGCATT CTGGGATCAGTTTTACAGTGAAACCCTAGGAAGGCCCACAACGTTCTGTAAGACTGTGGGGAAAGCTGAC ATTTGGCTAATCCGAACATATTGGGATGTTGAGTTTCCTCGTCCATATTTACCAAATTTTGAGTTTGTGG GAGGACTGCACTGCAAATCTGCCAAGCCTTTACCTAAGGAAATGGAAGAATTTGTTCAGAGCTCTGGAGA ACATGGTGTAGTAGTATTTTCACTGGGGTCAATGGTCAAAAACCTGACAGAAGAGAAAGCCAACCTCATT GCCTCTGTCCTTGCCCAGATTCCCCAGAAGGTTTTGTGGAGATACTCAGGCAAGAAGCCAGCCACATTAG GATCCAATACTCGGCTTTTTAATTGGATTCCCCAGAATGATCTTCTTGGACATCCTAAAACCAAAGCTTT CATCACACATGGTGGAACAAACGGGATTTATGAAGCCATTTACCATGGGGTCCCTATGGTGGGCGTTCCC ATGTTAGGGGATCAGCCTCACAACATCGCTCACATGGAGGCCAAGGGAGCAGCCCTGAAAGTCAGCATCA GTACAATGACGAGCACAGATTTACTCAGTGCTGTGAGGGCAGTCATTAATGAGCCTTCTTATAAAGAGAA TGCCATGCGGTTATCAAGAATCCACCATGATCAGCCAGTGAAGCCCCTGGACCGAGCAGTCTTCTGGATT GAGTTTGTCATGCGTCACAAAGGAGCCAAGCATCTTCGTGTGGCAGCCCATGACCTCAGCTGGTTTCAGT ACCACTCCCTAGATGTGATTGGGTTCCTATTGTTGTGTGTCGTTACTCTGACATTCATCATCACTAAATT TTGTTTGTTTGTGTGTCAAAAACTTTATATGAAAGAAAGTAAGAAAATGGGGAACAGAAAGAAAAAGAAC TAGGTCTTTTTTAGGTTTGGGAAAGCCCTGAGTGACAATTATATTAACAATCACCACAAGAAGCATGCAA CTTCCTGCTTTATACCTATTTTCAAATAAGCAGCTCTGTTTCAGACTGGAAATAAATGTAAACATTAAGC ATGATAATTAACTACTGATTTGTTCCAATCTTCTATCTTGTAGGCATTCTCCTCTCACTCTTCTAAGATA TGGAAAATTAATTCTAAATATATTCTATAGAAAGGAAGTGATGAATAACAATATGCTAGACCTCTGGGAA GACATATAATACCAATCTTATTGATGTATATGAGGATCTCTTACAGTCAATGTCATAAGCCTATCATATT GTCTAAGAACAACCAAAAGAATTTAGTGTGAGAGGGTACTTTTCTAATAAGAAGGAACGTTTCTGTTTTT ATAGTGTTGCAGATATTAGAGCATGATTTCTTTTTTATTTTCCAAAAATAATTTATTGAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:5133743 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5133743 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14167 same embryo
	EMAGE:14165 same embryo
	EMAGE:14168 same embryo
	EMAGE:14166 same embryo
	EurExpress:euxassay_001860 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source