EMAGE mouse gene expression spatial database

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EMAGE:14166

Serpina1d serine (or cysteine) peptidase inhibitor, clade A, member 1D ( MGI:891968)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001859. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001859_01	euxassay_001859_02	euxassay_001859_03	euxassay_001859_04

euxassay_001859_05	euxassay_001859_06	euxassay_001859_07	euxassay_001859_08	euxassay_001859_09

euxassay_001859_10	euxassay_001859_11	euxassay_001859_12	euxassay_001859_13	euxassay_001859_14

euxassay_001859_15	euxassay_001859_16	euxassay_001859_17	euxassay_001859_18	euxassay_001859_19

euxassay_001859_20	euxassay_001859_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14166_wholemount_strong.wlz

14166_wholemount_moderate.wlz

14166_wholemount_weak.wlz

14166_wholemount_possible.wlz

14166_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14166_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

strong

regional

strong expression: see section 09 10 11 12 13 14

pancreas

strong

regional

strong expression: see section 06 07 08 09

hindgut

strong

regional

strong expression: see section 09 10 11 12 13 14

rectum

strong

regional

strong expression: see section 10

midgut

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4936
Entity Detected:	Serpina1d, serine (or cysteine) peptidase inhibitor, clade A, member 1D ( MGI:891968)
Sequence:	sense strand is shown >T4936 GACCCACGCGTCCGCCCACGCGTCCGGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGGGTCTG CTGCTTCTGGCAGGCCTGTGTTGCCTGGTCCCCAACTTTCTGGCTGAGGATGTTCAGGAGACAGACACCT CCCAGAAGGATCAGTCCCCAGCCTCCCATGAGATCGCTACAAACCTGGGAGACTTTGCCATCAGACTATA CCGGGAGCTGGTCCATCAGTCCAACACTTCCAACATCTTCTTCTCCCCAGTGAGCATTGCCACAGCCTTT GCTATGCTCTCCCTAGGGAGCAAGGGTGACACTCACACGCAGATCCTAGAGGGCCTGCAGTTCAACCTCA CACAAACAGCAGAGGCTGACATCCACAAGTCCTTCCAACACCTCCTTCAAACCCTCAACAGACCAGACAG TGAGCTGCAGCTGAGCACAGGCAATGGCCTCTTTGTCAACAATGACCTGAAGCTGGTGGAGAAGTTTCTG GAAGAGGCCAAGAACCATTATCAGGCAGAAGTCTTCTCTGTCAACTTTGCAGAGTCAGAGGAGGCCAAGA AAGTGATTAATGATTTTGTGGAGAAGGGAACCCAAGGAAAGATAGTTGAGGCAGTGAAAGAACTGGACCA AGACACAGTTTTCGCCCTGGCGAATTACATTCTCTTTAAAGGCAAATGGAAACAGCCATTCGATCCTGAG AACACTGAGGAAGCTGAGTTCCATGTGGACGAGTCCACCACAGTGAAGGTGCCCATGATGACCCTCTCGG GCATGCTTGACGTGCATCATTGCAGCATGCTGTCCAGCTGGGTGCTGCTGATGGATTACGTAGGCAACAC CACTGCTGTCTTCCTCCTGCCCGATGATGGGAAGATGCAGCATCTAGAGCAAACTCTCAACAAGGAGCTC ATCTCTAAGTTCCTGCTAAACAGGCACAGAAGCGATGCTCAGATCCACTTCCCCAGACTGTCCATCTCTG GAAACTATAACTTGAAGACACTCATGAGTCCACTGGGCATCACCCGGATCTTCAACAATGGGGCTGACCT CTCCGGAATCACAGAGGAGAATGCTCCCCTGAAGCTCAGCAAGGCTGTGCATAAGGCCGTGCTGACCATC GATGAGACAGGAACAGAAGCTGCAGCAGCCACAGTCTTACAAGCAGCTACTTATTCTATGCCCCCTATCC TGCGCTTCGACCACCCTTTCCTTTTCATAATATTTGAAGAACACTCTCAGAGCCCCCTCTTTGTGGGAAA AGTGGTAGATCCCACACATAAATGACCACCCTAAGATGTCATCCTTCCTTCTGAATTGGGTTCCCTTCCA TTAAACACAGGCTGGCCTGGCTCGTGCCTGATGCTACTGCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5125194 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5125194 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14167 same embryo
	EMAGE:14165 same embryo
	EMAGE:14168 same embryo
	EMAGE:14164 same embryo
	EurExpress:euxassay_001859 same experiment
	MGI:4827977 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source