EMAGE mouse gene expression spatial database

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EMAGE:14195

A230050P20Rik RIKEN cDNA A230050P20 gene ( MGI:2441788)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003988. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003988_01	euxassay_003988_02	euxassay_003988_03	euxassay_003988_04

euxassay_003988_05	euxassay_003988_06	euxassay_003988_07	euxassay_003988_08	euxassay_003988_09

euxassay_003988_10	euxassay_003988_11	euxassay_003988_12	euxassay_003988_13	euxassay_003988_14

euxassay_003988_15	euxassay_003988_16	euxassay_003988_17	euxassay_003988_18	euxassay_003988_19

euxassay_003988_20	euxassay_003988_21	euxassay_003988_22	euxassay_003988_23	euxassay_003988_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14195_wholemount_strong.wlz

14195_wholemount_moderate.wlz

14195_wholemount_weak.wlz

14195_wholemount_possible.wlz

14195_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14195_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4998
Entity Detected:	A230050P20Rik, RIKEN cDNA A230050P20 gene ( MGI:2441788)
Sequence:	sense strand is shown >T4998 GCGGTGAGGCGCGCTCGGAGGCCCGGGAGCACCACCCGGGAGCACTACAGGGCGCGTGAGCAGACGCGTG GCCGCAAGGCCTGCGCGGGGTCTCCTCAGGGTCCCCAGCTTTACGTAGAAACGAAACTGAAAGAGGGGCC CGAGGGAGGCGGAGCTGCGGCGACGCCACCCAGGCTTGCGAGGCCCGGACCCAGGCGCAGCCAGGTGTGC TCCGGCCACCATGGCTCAGGATGGCGTGGAGTTGGAGAAGAGCGTCAGGCGCCTCCGGGAGAAGTTTCAT GGGAAAGTGTCCCCCAAGAAGGCAGGGGCTCTTATGAGGAAGTTTGGCAGCGACCACACCGGAGTTGGGC GCTCTATCGTGTATGGTGTCAAGCAGAAAGACGGACAGGAGCTGAGCAACGACCTGGACGCTCAGGACCC ACCAGAGGACATGAAGCAAGACCAAGATATCCAGGCAGTAGCCACCTCTCTGTTGCCCCTGACGCAAGCC AATCTTCGAATGTTCCAAAGAGCCCAAGATGACCTCATCCCTGCCGTGGACCGACAGTTTGCCTGCTCCT CCTGTGACCATGTATGGTGGCGCCGTGTACCCCAGAGGAAAGAGGTGTCACGGTGTCGCAAGTGTAGGAA GCGTTACGAACCAGTGCCACTTGACAAGATGTGGGGCCTGGCAGAGTTCCACTGCCCAAAGTGTCGCCAC AACTTCCGGGGCTGGGCACAGATGGGGTCCCCATCACCCTGCTACGGGTGCGGCTTCCCTGTGTACCCCA CCCGGATCCTGCCCCCACGCTGGGACCGTGACCTGGACCGTCGCAGCACACACACCCACTCCTGCTCAGC TGCAGATTGCTACAATCGGAGAGAGCCCCATGTGCCTGGGACCTCCTGCGCCCACCCCAAGAGCCGTAAG CAGAACCACCTCCCCAAAGTGCTGCACCCGAGCAACCCCCACATCAGCAGCGGCTCCACGGTGGCCACGT GCCTGAGCCAAGGAGGCCTCGTGGATGACCTGGACCACCTCATCCTGGAGGACCTGAAAGAGGAGGAGGA GGAGGAGGAGGAGGAAGAGGAGGACGGTGGGCCCCGAGAGTGACCCTGGCTAGGTGCACACAGGAAGACA GACACAGTGTGTGGCCACCTCGTGTTGCTATAAGATACCAGCTGAAGGAGATAGGAACACCCTTGGGGGT ACTGATGGGACAGTCACAAGCGCACAAGCCGGGTCAACATAACAGCTTTCTTCAGAGAGTACGCCCCACC ACGGAACTCGGCCCAGTAGACCCCGTCCTGGTATCGGCTCCGGTAATGACCTCCATGATACCATACTCCA TTGAGGTTAGAGTGGCACAGGCATGGTACCACCAGCCCCCACGATGGTACAGGGCACAGTTACCTGAGGG AGAGGGAGAGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTTCG ATTTTTTTCACCAAAATAAAAGTCTCTACCAGCACTTCTTTGTGCAAAGCTTTTGCAAGCATTCCTCAAC CTTCCCTATCTTATTGAATAGAAGCCCTGCTACTCCATCTCTTCACCGAAATAAAAGGCTCTGGCTTCTC AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5151846 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5151846 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14200 same embryo
	EMAGE:14197 same embryo
	EMAGE:14199 same embryo
	EMAGE:14198 same embryo
	EMAGE:14196 same embryo
	EurExpress:euxassay_003988 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source