EMAGE mouse gene expression spatial database

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EMAGE:14200

Rchy1 ring finger and CHY zinc finger domain containing 1 ( MGI:1915348)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003997. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003997_01	euxassay_003997_02	euxassay_003997_03	euxassay_003997_04

euxassay_003997_05	euxassay_003997_06	euxassay_003997_07	euxassay_003997_08	euxassay_003997_09

euxassay_003997_10	euxassay_003997_11	euxassay_003997_12	euxassay_003997_13	euxassay_003997_14

euxassay_003997_15	euxassay_003997_16	euxassay_003997_17	euxassay_003997_18	euxassay_003997_19

euxassay_003997_20	euxassay_003997_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14200_wholemount_strong.wlz

14200_wholemount_moderate.wlz

14200_wholemount_weak.wlz

14200_wholemount_possible.wlz

14200_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14200_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5002
Entity Detected:	Rchy1, ring finger and CHY zinc finger domain containing 1 ( MGI:1915348)
Sequence:	sense strand is shown >T5002 GCCCGCCAGACGACGCAGCGGTCCCCTTCGCCCGCAATCAGGGCTGGCAGCGATGCCGGGGCGGGGAAAA AGAGCCAACCTAAGACTTACTACCATTAGCAGTGACGACAGCGACTAACGTGGACGACGGACTAAGCGTC TCACTTGGCTTCCACCCCTTACACCAGGGACCGGAAATCCGGATGCTGCAGATCACGTGATTGGACCCCC CCTTCCTGACTCTGGTGGCCGCTGCTCCGAGGAGGAGCGGGTGTCCGACCGACTCTGCTTCCTTAAGGCG TGGCTTCCAAGCTGGGGGAAAGGTTCAGTGGCTGGTGAGGGGGAGGTGGTGGAGGAGGAGGAGGAGGAGA TGGCGGCGACGGCGCGGGAAGATGGCGTCCGCAACCTGGCGCAGGGGCCGCGGGGCTGCGAGCACTATGA CAGAGCTTGTCTCCTCAAGGCACCTTGTTGTGACAAGCTTTATACCTGCCGGCTGTGTCACGATACCAAT GAAGATCATCAGCTAGATCGTTTCAAAGTCAAGGAAGTGCAGTGCATCAACTGTGAAAAACTCCAACATG CCCAGCAGACTTGTGAAGACTGTAGCACATTGTTTGGAGAATATTACTGCAGTATTTGCCACCTGTTTGA CAAAGATAAGAGGCAGTACCACTGTGAGAGCTGTGGGATTTGTAGGATTGGTCCAAAGGAAGATTTTTTC CATTGCTTGAAATGTAATTTATGCCTAACCACGAATCTTCGAGGAAAACACAAGTGTATTGAAAATGTTT CCCGGCAGAATTGTCCAATATGCTTGGAGGACATTCACACCTCCCGTGTTGTTGCTCACGTCTTGCCGTG TGGGCATCTCCTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGTTACAGATGCCCATTGTGTATG CATTCTGCCTTAGACATGACTCGGTACTGGAGACAACTAGATACTGAGGTAGCACAGACTCCCATGCCAT CCGAATACCAGAACGTGACTGTTGACATTCTCTGCAATGACTGTAATGGACGATCCACAGTCCAGTTCCA TATCTTAGGCATGAAATGTAAGCTTTGTGACTCCTACAATACTGCCCAAGCTGGAGGCCGTAGAGTGCCA GTGGATCAGCAGTGAGAAGCCTGTCCTTGGCTAGAGAACAGCTCTGTGATGGAGATGAAGCTCTGTAAAA GATCCCAGTGTTCGTTCATCTGCATCAGAGATGTTTCATACCACTGTCACAACAGGGGGTCACAGCGTGA ACACCTGAGGATTCAAGATCTCTGGATGGAAATGTAATAGAGCTTACTGGGTGATTGTAATAGCGATTAC TGGGTGAAGCCTCTGAGCGTGTGTAGATTGGAAATTCCTTTAGTGTCAAGTTTTGAAGCCAATGATGTTA GTCCCAGATATAGTCACATTCATCGAAATATTTTATATTTTCATGGAACTTTAAGTGATACTATGCACGC AGAGTTAATTTCTTGTTCTTGATAGAACTACATTCAATTTTAACTAGTCTTTGATTTTAAGGGCTGTACT GTGATTTTTAAGTTCTTATTAAAATCTAATTACGTGTGCTTTTTAATTTTCTTTTCTTTTCTCTCTCTCT CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGTTTTTTTTTGGTTCTTCCAGACAGGGTTTCTCTG TGTAGCCCTGGCTGACCTGGAGCTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGCCT CTGCCTCCCAAGTGCTAGGATTAAAGGCATGTGCCACCACGCCCGGCTGCTTTTTAATTTTCTAAGACAG TCCTTTGAATACTATAATACAATTTTATGTAATAAATTACTAATGAAATTGTACTGAAGTACTGTTTTAC AGACTTTTTTCCCCTCTAGGTTGTTAATTTTTGGCTCTTGTGACAACATAAGCCATCATATATAAAAGAC ATTTTCCCTTTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5249543 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5249543 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14195 same embryo
	EMAGE:14197 same embryo
	EMAGE:14199 same embryo
	EMAGE:14198 same embryo
	EMAGE:14196 same embryo
	EurExpress:euxassay_003997 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source