EMAGE mouse gene expression spatial database

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EMAGE:14201

Sel1l sel-1 suppressor of lin-12-like (C. elegans) ( MGI:1329016)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004016. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004016_01	euxassay_004016_02	euxassay_004016_03	euxassay_004016_04

euxassay_004016_05	euxassay_004016_06	euxassay_004016_07	euxassay_004016_08	euxassay_004016_09

euxassay_004016_10	euxassay_004016_11	euxassay_004016_12	euxassay_004016_13	euxassay_004016_14

euxassay_004016_15	euxassay_004016_16	euxassay_004016_17	euxassay_004016_18	euxassay_004016_19

euxassay_004016_20	euxassay_004016_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14201_wholemount_strong.wlz

14201_wholemount_moderate.wlz

14201_wholemount_weak.wlz

14201_wholemount_possible.wlz

14201_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14201_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4097
Entity Detected:	Sel1l, sel-1 suppressor of lin-12-like (C. elegans) ( MGI:1329016)
Sequence:	sense strand is shown >T4097 GGAAGAGCGGCTGCGAGGCGGCGGCACAGAGGCAGCGGCTGAGTTGGTCGCGGCGACGGCGACGGCGACG GCGAGGGCTCTCGACCTTCGAGAGCAGGATGCAGGTCCGCGTCAGGCTGTCGTTGCTGCTGCTCTGCGCG GTGCTCCTGGGCTCGGCAGCCGCGACCTCGGATGACAAAACTAACCAGGATGACTCCTTAGATTCCAAGA GTTCTTTGCCCACAGATGAGTCAGTGAAGGACCACACCACCACGGGCAAAGTAGTTGCTGGCCAGATATT TGTTGATTCTGAAGAAGCAGAAGTGGAATCCCTTCTTCAGGACGAGGAAGATAGCTCCAAGACCCAGGAG GAAGAGATCAGCTTTTTAGAATCTCCGAATCCAAGCAGCAAGACCTACGAAGAACTAAAGAGAGTGCGGA AGCCAGTCTTGACTGCCATTGAAGCTGAAGAAGATGCTGCCAAAAGACGACAGATGCAGGAAGCAGAGAT GATCTATCAGGCCGGGATGAAGATACTGAATGGAAGCAATAGGAAGAGCCAAAAGAGAGAAGCATATCGG TACCTTCAGAAGGCAGCAGGCATGAATCACACCAAAGCCCTGGAGAGAGTGTCCTATGCTCTCTTGTTTG GTGATTACCTCACACAGAATATCCAGGCAGCCAAAGAGATGTTTGAGAAACTGACTGAGGAAGGGTCTCC CAAAGGACAGACTGGTCTTGGCTTTCTCTACGCTTCTGGGCTTGGTGTTAATTCAAGTCAGGCAAAGGCT CTTGTATATTATACTTTCGGAGCTCTTGGAGGCAACCTGATAGCCCATATGATTTTGGGTTACCGCTACT GGGCTGGCATCGGAGTCCTCCAGAGTTGTGAGTCGGCACTGACCCATTATCGTCTTGTTGCCAATCATGT TGCTAGTGATATCTCCCTAACTGGAGGCTCTGTAGTCCAGAGAATACGGCTGCCCGATGAAGTGGAAAAC CCGGGGATGAACAGTGGGATGCTGGAAGAAGACCTGATTCAGTATTACCAGTTCCTAGCTGAGAAGGGTG ACGTCCAAGCACAGGTTGGCCTGGGACAGCTGCATCTGCATGGAGGGCGTGGAGTAGAACAGAATCACCA GAGAGCGTTTGACTACTTCAACTTAGCAGCAAATGCTGGCAATTCACATGCTATGGCCTTCCTGGGAAAG ATGTATTCTGAAGGAAGTGACATCGTACCTCAGAGTAATGAGACGGCACTTCACTACTTTAAGAAAGCTG CTGACATGGGCAACCCCGTGGGACAGAGCGGGCTTGGAATGGCCTACCTCTACGGAAGAGGCGTTCAAGT TAATTATGACCTGGCCCTCAAGTATTTCCAGAAAGCTGCTGAGCAAGGCTGGGTGGACGGGCAGCTGCAG CTCGGCTCTATGTACTACAATGGCATTGGAGTCAAGAGAGATTATAAGCAGGCCTTGAAGTATTTTAATC TGGCTTCTCAAGGAGGCCATATCTTGGCTTTCTATAACCTCGCACAGATGCACGCCAGCGGCACAGGGGT GATGCGGTCCTGTCACACTGCAGTGGAGTTGTTTAAGAATGTGTGTGAGCGAGGTCGCTGGTCAGAGAGA CTGATGACTGCCTACAACAGCTATAAGGATGAGGACTACAATGCTGCAGTGGTCCAGTACCTCCTGCTGG CTGAGCAGGGCTACGAGGTGGCGCAGAGCAACGCAGCCTTCATCCTCGACCAGAGAGAAGCAACCATTGT AGGTGAGAATGAAACTTACCCCAGAGCTTTACTGCATTGGAACAGGGCCGCCTCCCAAGGTTACACTGTG GCTAGAATTAAGCTTGGAGACTACCACTTCTATGGCTTTGGCACTGATGTGGATTATGAGACCGCATTTA TTCATTACCGCCTGGCTTCTGAGCAGCAGCACAGCGCCCAAGCTATGTTTAACCTGGGCTACATGCACGA GAAGGGCCTAGGCATTAAACAGGACATTCACCTTGCAAAACGCTTTTATGACATGGCAGCCGAAGCTAGC CCAGATGCACAAGTACCTGTGTTCCTCGCACTCTGCAAATTAGGTGTCGTCTATTTCTTACAGTACATAC GGGAAGCAAACATTCGAGATCTATTCACACAACTGGATATGGACCAGCTTTTGGGACCCGAGTGGGACCT TTACCTCATGACCATCATTGCACTGCTCTTGGGTACAGTCATAGCTTACAGGCAGCGACAGCACCAGGAC ATACCAGTTCCCAGGCCCCCAGGGCCACGGCCGGCTCCTCCCCAGCAGGAAGGACCACCAGAGCAGCAGC CACCACAGTAGCAGCCACACACTCACCTTGGTCGGTGACCACTGGGGAGCTGCTTGCTGAGAACGCTTGC ATTTGATGTAGGGCTCTGGATGGTGGCACCGCCTGGAAGAGGCGTGAGGCGTGTTGAATCCCAGAAGCTG CTTAGAACCTGCTGCCTTCCTTTTCAGGGATGCGCGGCTCTCGGCAGAGCTGCAGTGAATGTTTGTTTCA GTGCCATACGGAATGACAACTCTCAGTGGCTTTCCTCTTTCCTTTTTCTTTCTTCGTTCTGCAAACATAC ACAAGACACTCGAGTCATGTCTACTGTACCCTGTCTTTCCTGAGAGACCTTTCGTCATCCTGTTGATGTG CATAACTTCTTACCTGTCTTCTAAGGTCCTGAGCATTGACAACCTGGGGAAACCCAGTTAGTTGCAAGTT TAACCCTAAAAACATGCTTAAGTCTGAGACTAGAACTTGCCATTAAGTCAAAAGCAAAACAAGCCAAGCA GGACGTCTTCAGGGTGTTTGGGAAATGCAGTAACTGGGAAATTTTACCAGTCTACATGCCAGGTCATATG TTCGGCACGCTTATTTTAAAAGGGATTATTGATCGGATTCCTGTACTGTGACTCTGACACTTCCTAGCAA ACTTCTCTTCCTCAAAGACTGAAAGGCTTTGCTAAAGACTTCTTCGTGATGTCACATCCCTGCCATGGAG TAGAAGTCAAACTCAGTCAGCTGACTCCGTGCTCCCACAGAGGACTTTGCCCCAGCTACTCATGTGAGTT GCATATACAGAGCCCTAGTCCAGACGCCAGAGGATGATCTAAATGGAGAAATCAGAAAACAGCCTTAACC AACTTGAGGTGGGACCTGGGACATGCCCTCCCTCGGGTAGATTCGTTGCTTCCCGGATCTCATTTTACTT CACATTTCTTATGGCACAGAGAACAGACAACCCTCCCAAAGTCTTCAAATATGAAATGCTATTGTCAAGA TTGGCAGGTGGTGACTTCCTTTGTAATACTAAAATACTAAAATACTCAGACAATGGAGGATGGGTTAGAC CCAATGCATGGCAAAACCAAAGGCCTGGGTGCACCGTTGTGTATGCTGCGGGAGCTTTTTATTTCTGACG GTTTGATGTGGCTGTAGAAAGTCAGCCACTGAGTAGCTGTTGTGTTATCGCCCTTCTGTTGTAAACATTA GGAAATTAAAGCAGTTACACCCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4457637 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4457637 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14203 same embryo
	EMAGE:14202 same embryo
	EMAGE:14205 same embryo
	EMAGE:14204 same embryo
	EurExpress:euxassay_004016 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source