EMAGE mouse gene expression spatial database

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EMAGE:14204

Recql RecQ protein-like ( MGI:103021)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004015. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004015_01	euxassay_004015_02	euxassay_004015_03	euxassay_004015_04

euxassay_004015_05	euxassay_004015_06	euxassay_004015_07	euxassay_004015_08	euxassay_004015_09

euxassay_004015_10	euxassay_004015_11	euxassay_004015_12	euxassay_004015_13	euxassay_004015_14

euxassay_004015_15	euxassay_004015_16	euxassay_004015_17	euxassay_004015_18	euxassay_004015_19

euxassay_004015_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14204_wholemount_strong.wlz

14204_wholemount_moderate.wlz

14204_wholemount_weak.wlz

14204_wholemount_possible.wlz

14204_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14204_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4109
Entity Detected:	Recql, RecQ protein-like ( MGI:103021)
Sequence:	sense strand is shown >T4109 GGACGTTTGGATCTCCGGTTCAGTTTTAGAATGGCATCCGTCTCAGCTTTGACAGAGGAACTGGAATCCG TAGCCAGTGAGCTTCATGCCATTGACATCCAGATTCAGGAACTCACAGAGAGGCGGCAAGAGCTTCTTCA GAGAAAGTCAGTCCTGACAGGGAAAATCAAACAGTACTTGGAGGACTCTGCGGCTGAGGCGAGCAGCGAC TTGGACACATCACCAGCTGCTTGGAATAAAGAAGATTTTCCATGGTTCGGAAAGGTAAAAGATGTGCTGC AAAATGTTTTTAAACTGCAAAAATTCAGACCTCTACAGCTGGAAACCATCAATGTCACCATGGCTAGAAA GGATATCTTTCTTGTCATGCCCACAGGAGGAGGGAAGAGCTTATGCTACCAGCTACCTGCGCTGTGCTCA GACGGTTTTACACTTGTGATTTGCCCACTCATCTCTCTTATGGAAGATCAGCTGATGGTTTTAAAACAGT TGGGAATTTCAGCTACTATGTTGAATGCTTCCAGCTCTAAGGAACATGTAAAATGGGTGCACGCTGAAAT GGTGAATAAAAACTCCCAGTTAAAGCTGATTTATGTGACCCCAGAGAAAATTGCAAAAAGCAAGATGTTC ATGTCAAGACTGGAGAAAGCCTATGAAGCCGGGAGGCTGACGGGAGTGGCAGTGGATGAAGTGCATTGCT GCAGTCAGTGGGGTCATGACTTCAGGCCTGATTATAAGGCTCTTGGCATCTTGAAGCGCCAGTTTCCCAA CGCCTCACTGATGGGGCTGACTGCAACAGCAACCAACCATGTTCTGAAGGATGTACAGAAAATCCTGTGT GTGGGAAAGTGTTTGACTTTTACAGCGTCTTTCAACCGGCCAAATCTTTTTTATGAGGTTCGGCAAAAGC CCTCAAGTGCTGAAGACTTTACTGAGGACATTGTCAAGCTAATTAATGGACGGTACAAAGGACAATCAGG AATCATATACTGTTTCTCTCAGAAAGACTCTGAACAAATTACCATCAGTTTACAGAAGTTGGGAATTCAT GCAGGCACTTACCATGCCAATATGGAACCAGAAGATAAGACCAAGGTTCATACACAGTGGTCAGCCAATG AGCTTCAGGTGGTAGTGGCAACAGTCGCATTTGGCATGGGAATTGATAAGCCAGACGTGAGGTTTGTCAT CCATCATTCAATGAGCAAATCCATGGAGAATTACTATCAAGAGAGCGGGCGTGCCGGTCGCGATGACTCG AGAGCAGACTGTATTCTGTATTACGGCTTTGGGGACATATTCAGGATCAGCTCGATGGTGGTCATGGAGA ACGTGGGGCAGCAGAAGCTGTATGAGATGGTGTCATACTGCCAGAATGTCAGCAAGTGTCGCCGTGTGTT AATAGCACAGCATTTTGATGAAGTGTGGAACGCAGATGCCTGTAACAAAATGTGTGACAACTGCTGTAAA GACGTTTCGTTTGAGAAAAAGAATGTGACACAGCACTGCCGGGACCTGATAAAGATTCTGAAGCAGGCTG AAGGCCTGAATGAAAAGCTGACCCCGCTGAAGCTGATTGATGCTTGGATGGGAAAGGGAGCAGCCAAGTT GAGAGTGGCTGGCGTTGTTGCCCCGGCCCTTCCCCGGGAAGACCTGGAGAGAATCGTCGCCCACGCCCTC TTGCAGCAGTATCTCAAAGAAGACTACAGCTTCACGGCTTACGCCACCATCTCCTATCTGAAGGTGGGAC CCAGAGCTTGTCTTCTCAGCAACGAAGCCCATGCTGTCACTATGCAAGTGAAGAAGTCAGCACAGAGCAG CGTTAGGGGTGCACCGTCTGAAGCTCGTCAGGTGGAACAAGTGGATTCGAAGGGGGAAGAACAAAGCTCA GGTAACTCCCAGAAGTCTAAAAGCAGGCTTCAGCCATCTGGCTCTAAGAACGCAGGCGCTAAGAAAAGAA AACTTGATGATGCTTGAGTGTCACGTAATATTGTACAATGTAACTGTTCTGTATGTTCATGTCTGGGTCA TTTTTATAATCTGCTATAATTTTTGAAACAACTTCATGTTTTATACATATAAAGCTGTATTTTCAGAGCT TACCTTATACAATAATGAAAAATACAACTTTTATGTAAAGTTGTCTAAATGTAATATATCTCACATTAAG TCCACATAACTTTTGTAAGCCCGTTCGCCTTGCTGTAACTAGCTGTTGTCTTCTGACGTGACTCTCCTTT AAAGCAGTACTATGCTGACATGGGCTCACCTTTCCAAATGGGTGACATTTTAAGCTGAGCAATGTAGATG ACTTCTAGTGAGAAAAGTTGCTGTTATAATTCAGATAAAACAGTCCTCAGTACCAAGTCAGCAAGAATGG CCTGCATATTCTAAGTAAATCACGACAGCAGCCTCCTGAGGCCGCAGCCTGACTTTGACGTTCAGGCAAA CAAAGGAGCTGGTGTATTTGTATGAGTCAAGGTTTATCGTCAACTCTAGAGGAGGAGTGTTCATCTGCAA ATGAGTCATTAAACGAATTCATCCGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4459244 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4459244 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14203 same embryo
	EMAGE:14202 same embryo
	EMAGE:14205 same embryo
	EMAGE:14201 same embryo
	EurExpress:euxassay_004015 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source