EMAGE mouse gene expression spatial database

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EMAGE:14328

Tollip toll interacting protein ( MGI:1891808)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004170. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004170_01	euxassay_004170_02	euxassay_004170_03	euxassay_004170_04

euxassay_004170_05	euxassay_004170_06	euxassay_004170_07	euxassay_004170_08	euxassay_004170_09

euxassay_004170_10	euxassay_004170_11	euxassay_004170_12	euxassay_004170_13	euxassay_004170_14

euxassay_004170_15	euxassay_004170_16	euxassay_004170_17	euxassay_004170_18	euxassay_004170_19

euxassay_004170_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14328_wholemount_strong.wlz

14328_wholemount_moderate.wlz

14328_wholemount_weak.wlz

14328_wholemount_possible.wlz

14328_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14328_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7831
Entity Detected:	Tollip, toll interacting protein ( MGI:1891808)
Sequence:	sense strand is shown >T7831 CGCAGCTTGTGGTGGGAGCGGAAGAGCTCTCCCGGCGGGTCTCTGTGCAGTTGGCGAGGCAGAGGGCCGC CGCAGCACCATGGCGACCACCGTCAGCACGCAGCGCGGGCCGGTGTACATTGGTGAACTCCCCCAGGACT TCCTCCGTATAACACCCACACAACAGCAGCAGCAGATCCAGCTGGATGCCCAGGCAGCCCAGCAGCTGCA GTATGGAGGAACAGTTGGTACCGTGGGTCGCCTCAGCATCACTGTGGTACAGGCAAAATTGGCAAAGAAT TATGGCATGACTCGTATGGACCCTTACTGCCGTCTGCGTCTGGGCTATGCTGTTTATGAAACTCCCACAG CACACAATGGGGCCAAGAACCCTCGCTGGAACAAAGTCATTCAGTGCACAGTGCCCCCAGGTGTGGACTC GTTCTACCTTGAGATCTTCGATGAGCGCGCCTTCTCCATGGATGACCGCATAGCTTGGACCCACATCACC ATCCCCGAGTCCCTGAAGCAGGGCCAAGTGGAGGACGAGTGGTATAGCCTGAGTGGGAGGCAGGGCGATG ACAAGGAGGGCATGATTAACCTGGTCATGTCCTACACGTCCCTCCCAGCTGCCATGATGATGCCTCCTCA GCCTGTGGTCCTGATGCCAACTGTGTACCAGCAGGGTGTTGGCTATGTGCCCATTGCAGGGATGCCTGCT GTCTGCAGCCCTGGCATGGTGCCGATGGCCATGCCCCCACCAGCTGTGGCTCCTCAGCCCCGCTGTAATG AAGAGGACCTCAAAGCTATCCAGGACATGTTTCCCAACATGGACAGGGAAGTAATCCGTTCTGTGCTTGA AGCCCAGAGAGGGAACAAAGATGCTGCCATCAATTCCTTGCTGCAAATGGGTGAAGAGTCCTAGACCCGC CTGTGGCCCTTGGCTTCACACCACTGACCCTTGGACTTGTCCACCCAGGGCTCCCCAGGGTCACCATCCC CACAAGATTCCAATGAAAGACCATCCACATACCCCTTCCTTGGACATCTGCGCCGCCCCTTCCTACATAT TTCGGGGATGCATGTGGATTTTTGGTTCTAAGCAGGCAGTCCTCTTCATTGTTGCTGGGGGACAGAGTTA GGGGACAGTTCTCTCCTAGTCTCTAGCTGGGTGATGGTGGCATGGCCCTGTTATTGCACAGATCAGTGGT CCTGTTTGGGGACCTGCATCTCCTGTCCTCACCCTATTCTGGAAAGCATTCTTACAGAAAGCAACTGCCT CTGCCAAGATACTAGAAATTGTACAGCATCCTTCTGAGTTGATGGATGGATGGCATGGAGTTACTCAGTC CAGCTGGGATGGTTGTTGAGGGTGCACTGAGTTCATGTGCACTTACTTACAGGTTATTTTTAGAACTTTC AAGCTAAAAGCTCCTTCCCTCTTCTGATGTTGTATGTGAACATGGGCCCCTCAGCTAGTCCTGGCCAGTG AGATATGCTTCCCAGCCATATCCAGTGGGTCTCTAACCTCCTGTCTAGCTTATCTGCCACTGTCTGGCTA GGCTTCAGCCACAGAAATCCAACTTGAATACAGAAGGTATCCAGACCTGGTGCTTGGTTGGCCAGGCCCC ACATTAGCCCTCACTGCTGGAGGCTGCTTTCCACATCCTCTTGTTTAGTACCAGCATCATGGGTCTTACT CCAGGGGCTGTTCTTGTTGATAGACCTTCATCACAGAGCCCAGAGTCAAGCGAAGGAGATGGGTGGGTGT GGCACTATGTGCTTACTACTAAAATTCAAGCATTGTGACACTACTGACCTGCAGTATACTCTGTGGACCC ATACTGTAGCTTGGCTTGAGTGATAAAAATTCATGCTTAAATTAAACTTTCATAAAGGTAGAGGTGCCTG TACCTGACCAGTGGATATTGTATAGTGTATTTTAGGTTGCAAACCTTTGAGCCCGTGACCTGGCCATACA TGTATTTAGGGAGAAATTGAGTGGTGCCTTGTGCTAAGATACAGACCGTGAAAGCTTTCTCAGAACCAGT ACTGTCACAAGGTGCTGTTAGGAAGCTCCAGCATTGCCACAGGGTTATGGTGGGACAGTTTTGCCTTACA TTGCCGTTACTTTTTGTTTTCTGAGAATCTGCACACCCGGTTTGGATATGTGATCCACCAATAAGGGCAT GAAGGTGACTCCTAGCAGTTTGGACTGTTGTGAAGGACTTCAAATAAGAAAATTAGTGCTTCAGTTTTTG GTCTTTGCCTCCTAAAAACGCGGTAAAATATCCTACTTGCTGTTATCTACAGGGGTGCTTTTTGTCAAAT GTATTGTTTATAAGTATTTATTTTTGTAAGCCTAAAGGCAGTATGTGGTGATGGTGAGCATGGGATGGTG GTAACAAGCACTTTTCTTTAGTTGTAGGTCTTTAAACCTTTTAACTGCAAAATGGAGAAGAAAGACCAAT GTTTATTAAATAGATTCAACCACTTACTTTAACACAACCAGGCACTGGAAGCCTCCATTCTAGACTGTTG ATCGGCCTTGTGCTTGGTAAGCAGATGCCCTCTTGCAGGGTGGATGAGTCAGGCAGGCAGAACCTCCTTA AGTGGTCCACTTAGTGTTACCAGCAGACTGCAGACACTGCAGAGCAGGAACCTCACTCAGTACCTGTCTC TAGGTCTCAGGTGTGGCGGCTGTGTGCATCCGCATCTGCCTTACGGAGGTATAGGTTGAACACAGGGCCA CTTTGTTCTGTGAGGTTCTATAGGAAGCATCACGATTTGCTGCCAGCCCCTGAGCAGTCCCACAGCACTC AGAGGCAGCGTTCACTAACTTACGACTGCTTAAATGAGGAAGAGTGATGGTTTTAAGACAGATTTAAGAC TTGAGCTGAACACAGACTCTTCCCTTTGTCATGAACATTTTATTTTTTTAATAGATTGAAGAAAGATCAC ATTTTATAAGGCCTGAGTGATACTCCTAGGTTTTGCTGTTTTTTTGTCTTGGAATTTCATGTCAGCGCCC ATCTAGTGCAAGGAGAGAGGCAGCACTTGTCAGCGCCTGTGTAGGTACCAGGGCATCAGAGCATGGCTTG TTGGCTTCCATCCCATCCTGAATCTTGTTGAGATGAAGAGTCATCCCTAGAAGGAGCTCAGAGGCAGAGG CCACAGCCGAGTCAGACAGGCTTCTGCATGGGGACCAGGCTCTCACTGGGTGTAATTGCTGCTCTTGCCA AGTTTGAAGATTGATGTTGGTAGCAAAATTTCTCAAGGTGTTCACTGGAGCCCCCTCCCCTCGGGCCAGC TCTAGTGTAGGCATGCATCTAGGGATGGCCTGGCCTGTTTTCTTCTCTGGACATGTTGGTTGGCTGAAGT TGTAACCTGCTGCACGCTTGTGGGCTGACATGGCCAGAGGCTTTCATCTCACCATGTGAGTGATGCCTAC CCGATGACAGAAGTGTTGTGCGACTGGGCTCTGCCCTCGCCCTCCCAGGCCACCCTTTACAGACACTGGT ATCATCTGGTACACTGTCTGGAGGTTGGTGGGAATGGTTCTCAGAACTGTCTGTGATTATTTTGGGAATG GGGGGGTCCATGTCCATATTTTTCTTTGATTATATGATATATTTTTACTTTTCAGCCTTCTAATTTAATA AGTGGTCTATATAAAATAGAAAAATAAAGTCCTCTGAGGAAATGTTAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6334946 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6334946 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14332 same embryo
	EMAGE:14330 same embryo
	EMAGE:14329 same embryo
	EMAGE:14331 same embryo
	EurExpress:euxassay_004170 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source