EMAGE mouse gene expression spatial database

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EMAGE:14330

Lhfpl1 lipoma HMGIC fusion partner-like 1 ( MGI:1891214)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004167. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004167_01	euxassay_004167_02	euxassay_004167_03	euxassay_004167_04

euxassay_004167_05	euxassay_004167_06	euxassay_004167_07	euxassay_004167_08	euxassay_004167_09

euxassay_004167_10	euxassay_004167_11	euxassay_004167_12	euxassay_004167_13	euxassay_004167_14

euxassay_004167_15	euxassay_004167_16	euxassay_004167_17	euxassay_004167_18	euxassay_004167_19

euxassay_004167_20	euxassay_004167_21	euxassay_004167_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14330_wholemount_strong.wlz

14330_wholemount_moderate.wlz

14330_wholemount_weak.wlz

14330_wholemount_possible.wlz

14330_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14330_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

spotted

weak expression: see section 06 07 08 09 10 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7848
Entity Detected:	Lhfpl1, lipoma HMGIC fusion partner-like 1 ( MGI:1891214)
Sequence:	sense strand is shown >T7848 CTGTCTGACAAGCTGTGAGAAGTTCTTATTTTCTCTCTCTCTCTTCTCAAATCTGAACCAGGCGAAAGGG AACAAGTCAATCAGCCTTCGTGGTTAGAAGCCTAGCAACTTAGAAGGAAAGGATGGTGAAAAGCAATATC TCACAAAGACCCAAATGACATTTGAGAGAACGATCTCCTCAAGAACAAGATCCATACAGCATACCTGTGA GTCCCAGGACCTGGCAACCTGATGAAAACAGTCCAGTGAAGCCTACATCATAACTTGATTTTTGCTGCTG TGAACCATCTCATCTCCCCATCCTTGCACAGAAACCCCGAGACCATGAGGAACAGCCTTACTATGGTAGG CACCTTCTGGGCCTTCCTGTCCCTAGTTACCGCTGTGGCCAGTTCTACCAGTTACTTCCTTCCTTACTGG CTGTTTGGATCACAGCTTGGAAAGCCAGTATCTTTCAGCACATTCAGGAGATGCAACTACCCTGTGCGGG GAGATGGGCACAATCTGATCATGGTGGAAGAATGTGGGCGCTATGCCAGCTTCACCGCCATACCAAGCCT GGCCTGGCAGATGTGCACGGTGGTGACAGGTGCTGGCTGTGCTCTGTTGCTTCTGGTGGCATTGGCTGCT GTCCTAGGATGCTGCATGGAAGAGCTCATCTCTAGAATGATGGGGCGTTGCATGGGAGCTGCACAGTTTG TAGGAGGGCTGCTGATAAGCGCAGGCTGTGCATTATATCCTTTAGGATGGAACAGCCCGGAGGTAATGCA AACATGCGGGAATGTCTCCAATCAATTTCAGTTAGGAACCTGTAGACTCGGCTGGGCCTATTACTGTGCT GGGGGTGGAGCTGCTGCAGCCATGTTGATCTGCACCTGGCTCTCTTGCTTTGCTGGAAGAAACCCCAAGC CAGTTATGTTGGTGGAGAACATCATGAGAAATACCAATTCTTATGCCTTGGAGCTTGATCACTGCCTCAA ACCTTAAGTTTTGAAAGATTTACTAAAGAGGGAAAAGAAACCTGAACAGATGTATTAAGTAAGCCAAGAT AGCCACCTACTAGTCGAATACCCTAATGCTTGCATGCTCCTTAAACATGTGTCACTCATTTCCATGTTCC CCCAAAACTCCAAGTCCATGGCTCTTTAGATAATTTTTCAGCACAATGATTCAAACATTCACGGGAATGA AGTTTCTACTGGTCACCCAAGTCACACTGAGTACCTTTATGCAAAGTAAGATTCTTACTTGAAAAGTTAC CAAATAACCAAGAAACTTCTCAGAGTTCTATAACATGTACTTCCCACTTACTTTAAAGCATAGATTCTGT TCAGGAGCAGTAGTACTCAAGGGGCACTGGAAGACACACTAAGCTATTTTGCACCCATTCATTTGCATGG CTCTCCCCCTAATATTTATTGTTCCTCACAAGCACATCACAAGAATTACTATTTGAAACATTTGTGAATT TCTCAAGCCTATACTTCATTTCCATTTCGTTTTTTTTTCTTTTCTTATGCACGAGAAAGCATGGTAGAAA GGTAACTTTCAGTGGGGAAAACACTAATCATGTTCACGTGCATCTAATATGGGGCAGCTTTCACATTATG CTTTAGAATATTCATAGGAGTTAATTTTCTACTCTTGCTGGGTGACTGGTATCAAAATATAGTGGCAAGT GTTCCTTAAACTATTCTAGCTAGAGGAATGTTGTTCATTGAGTCTAACATTTGTCAGGTGTTCAATAAAC ATGAACTCCCAGTGATGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6389932 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6389932 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14332 same embryo
	EMAGE:14329 same embryo
	EMAGE:14328 same embryo
	EMAGE:14331 same embryo
	EurExpress:euxassay_004167 same experiment
	MGI:4825918 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source